int. j. life sci. biotechnol. International Journal of Life Sciences and Biotechnology 2651-4621 International Society of Academicians Microbiology (Other) Mikrobiyoloji (Diğer) in vitro Antimicrobial Effects of Azadirachta Indica Seed Oil in vitro Antimicrobial Effects of Azadirachta Indica Seed Oil https://orcid.org/0009-0009-0149-7366 Emmanuel Adarju Adamawa State University, Mubi https://orcid.org/0000-0003-1089-7416 Sudi İsmaila Yada Adamawa State University Mubi 04 16 2026 9 1 250 260 06 26 2025 01 12 2026 Copyright © 2018, International Journal of Life Sciences and Biotechnology 2018 International Journal of Life Sciences and Biotechnology

The study was aimed to determine the antimicrobial activity of Azadirachtaindica seed oil against some disease-causing organism such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa. This study of antibacterial activity against selected pathogens was done by dilution method, agar disc diffusion method and agar well diffusion method. Pathogens were taken from General Hospital Mubi. The locally extracted oil tested showed antibacterial activity against all the three (3) pathogenic bacteria even at very low concentration. In case of two (2) of the pathogens (Staphylococcus aureus and Pseudomonas aeruginosa), inhibition of growth caused by Azadirachta indica oil was 99.99%. The results showed that the oil will be an honest source of antibacterial agents. The encouraging observation also indicate that this oil should be exploited as natural antibiotic for the treatment of several infectious diseases caused by these pathogens and could be useful in understanding the relations between traditional cures and current medicines.

The study was aimed to determine the antimicrobial activity of Azadirachtaindica seed oil against some disease-causing organism such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa. This study of antibacterial activity against selected pathogens was done by dilution method, agar disc diffusion method and agar well diffusion method. Pathogens were taken from General Hospital Mubi. The locally extracted oil tested showed antibacterial activity against all the three (3) pathogenic bacteria even at very low concentration. In case of two (2) of the pathogens (Staphylococcus aureus and Pseudomonas aeruginosa), inhibition of growth caused by Azadirachta indica oil was 99.99%. The results showed that the oil will be an honest source of antibacterial agents. The encouraging observation also indicate that this oil should be exploited as natural antibiotic for the treatment of several infectious diseases caused by these pathogens and could be useful in understanding the relations between traditional cures and current medicines.

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