Cytotoxic, apoptic and necrotics effects of Agrocybe cylindracea (DC.) Maire on breast cancer cells MCF-7 and L929

Year 2017, Volume: 1 Issue: 1, 32 - 38, 01.06.2017

Hayriye Baran Perihan Güler Mustafa Türk

Abstract

The cytotoxicity, apoptotic and necrotic effects of Agrocybe cylindracea were investigated.The tissue fragments from taken dry basidiocarps were developed by tissue culture method in malt extract agar.Three pellets were taken from the secondary mycelium and its development completed in 7 days at 140 rpm at 27 °C in nutrient broth.In cytotoxicity studies, sample extract concentrations were applied to MCF-7 and L929 fibroblast cells as 5mg/ml, 2.5 mg/ml, 1.25 mg/ml,0.625 mg/ml and 0.3125 mg ml. The WST-1 solution was added and the absorbance was measured in a microplate reader at a wavelength of 440 nm as a result of the incubation. Apoptosis and necrosis were detected in the same cell line as the double staining experiment. Binary staining solution was prepared and MCF-7 and L929 Fibroblast cells were applied at the same concentrations.At the end of the incubation,measurements were done at 20X magnification in a fluorescence microscope with FITC and DAPI filter

Keywords

Agrocybe cylindracea, Apoptotic effect, Cancer, Cytotoxicity, Necrotic effect

References

• Anonymus, 2017. Index Fungorum. http://www.indexfungorum.org/names/names.asp. (Date of access: 26.09.2017).
• Chien, R.C., Tsai, S.Y., Lai, E.Y.C., Jeng-Leun Mau, J.L., 2015. Antiproliferative activities of hot water extracts from culinary-medicinal mushrooms, Ganoderma tsugae and Agrocybe cylindracea (higher basidiomycetes) on cancer cells. International Journal of Medicinal Mushrooms, 17(5), 453-462.
• Fritsche, G, 1972, Experiments on the maintenance of strains of cultivated mushrooms. III. Propagation of by multispore culture. Mushroom News, 20(8), 4-19.
• Huang, S.J., Tsai, S.Y., Mau, J.L., 2005. Antioxidant properties of methanolic extracts from Agrocybe cylindracea. LWT Food Science and Technology, 39, 378-386.
• Liang,Y., Chen, H., Zhang, H.B., Jin, Y.X., Guo, H.Q., Chen, X.G., Sun, H., 2014. Lectin from Agrocybe aegerita as a glycophenotype probe for evaluation of progression and survival in colorectal cancer. APJCP - Asian Paciic of Cancer Prevention, 15(14) 5601-5605.
• Ooi , E.C.V., Liu, F., 2000. Immunomodulation and anti-cancer activity of polysaccharide protein complexes. Current Medicinal Chemistry, 7, 715-729.
• Rzayev, Z. M. O.,Turk, M., Söylemez, E. A., 2012. Bioorg .Med. Chem. 20, 5053-5061.
• Shon Y.H., Nam K.S., 2001a. In vitro cancer chemopreventive activities of polysaccharides from soybeans fermented with Phellinus igniarius or Agrocybe cylindracea. Journal of Microbiology and Biotechnology, 11, 1071-1076.
• Shon, Y.H., Nam, K.S., 2001b. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. Journal of Ethnopharmacology, 77, 103-109.
• Tsai, S.Y., Huang, S.J., Mau, J.L., 2005. Antioxidant properties of hot water extracts from Agrocybe cylindracea. Food Chemistry, 98, 670-677.
• Tsai, S.Y., Tsai, H.L., Mau, J.L., 2007. Antioxidant properties of Agaricus blazei, Agrocybe cylindracea, and Boletus edulis. LWT Food Science and Technology, 40, 1392-1402.