GST-Theta1 Enzyme Expression Levels in Brain Tumor and Their Relationship with the Clinical Data of the Patients

Year 2021, Volume: 5 Issue: 2, 82 - 98, 01.12.2021

Onur Dirican , Pınar Kaygın Sezen Yılmaz Sarıaltın , Can Yılmaz , Volkan Ateş , Gülçin Güler Şimşek , Serpil Oğuztüzün , Tülay Çoban Yusuf İzci

https://doi.org/10.47947/ijnls.1016468

Abstract

The most important drug metabolizing enzyme of the detoxification mechanism is known as Glutathione S-Transferase. GST enzymes may be associated with brain tumor epidemiology, clinical and demographic factors. The correlation between parameters such as changes in Glutathione S-transferase Theta1 proteins, tumor localizations, age, gender, alcohol use, smoking, chemotherapeutic/radiotherapeutic treatment status in normal and brain tumor tissues, diagnosed in neurosurgery department, were examined by immunohistochemistry. GST-Theta1 expressions were analyzed using immunostaining in samples from 149 patients diagnosed with brain tumors between 2016 and 2018. The mean age of the patient group was 49.44 years. 83 (58.45%) patients were male. After immunohistochemical staining, GST-T1 expression was found approximately 9,46 times higher in tumor tissues than in normal tissues (p<0.0001). Tumor tissues from patients who received chemotheraphy showed higher expression of GST-T1 than those who did not (p<0,05). In addition, GST-T1 expression level was observed at a significantly higher level in patients younger than 60 years of age compared to patients over 60 years (p<0.026; p<0.05). There was no statistically significant relationship between patients smoking behaviors, alcohol consumption, tumor localization and GST-T1 expressions. It is aimed to determine the GST-T1 protein expressions and to contribute to the examination of epidemiological and prognostic factors of brain tumors by comparing them with demographic and clinical data.

Keywords

Brain Cancer, Immunohistochemistry, Glutation-S Transferase, GST-T1.

Supporting Institution

Kırıkkale University Scientific Research and Projects Department

Project Number

2019/178

Thanks

Studies was carried out in the Kırıkkale University, Department of Biology, Tissue Research Laboratory. I thanks to all author. Also we thanks to University of Health Sciences, Gulhane Training and Research Hospital, Department of Pathology and Department of Neurosurgery and Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology.

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