International Journal of Pure and Applied Sciences 2149-0910 Munzur University Plastik Kompozit Destek Üzerine İki Endüstriyel Şus ile Oluşturulan Biyofilm Yapılarının Tespit Edilmesi The Determining of the Biofilm Form by Two Industrial Strains on Plastic Composite Supports Yonten Vahap Aktas Nahit 12 28 2016 2 2 50 56 12 30 2016 Copyright © 2015, International Journal of Pure and Applied Sciences 2015 International Journal of Pure and Applied Sciences

Bu çalışmada Candida intermedia NRRL Y-8278veKluyveromces marxianus NRRL Y-8281 endüstriyel mayalarının farklı PCS destekler üzerindeki birimalandaki koloni sayıları (CFU) geliştirilip, hesaplanmıştır. Farklı desteklerüzerindeki analiz yöntemi olarak Stripping-Sand metodu kullanılmıştır. Her birdestek için (PCS1, PCS2, PCS3, PCS4)hesaplamalar yapılmıştır. Kullanılan kültürlerin, plastik destekler üzerindebiyofilm yapıları meydana getirdiği tespit edilmiştir. K. marxianus’un PCS2 desteği üzerinde 2.9×108CFU g-1 ve 1.6×1010CFU mL-1 değerlerinde koloni oluşturduğu ve  C. intermedia’dan daha iyi biofilm performansı gösterdiği gözlemlendi.Kullanılan plastik desteklerin içerdiği polipropilen gıda, biyomedikal, hastaneve sağlık laboratuvarları gibi birçok yerde bazı cihazların yapısında bulunmaktadır.Ayrıca çalışmamızın sonuçları, polipropilen gibi bazı desteklerin veendüstriyel mayaların kullanıldığı gıda ile biomedikal teknolojileri veendüstriyel birçok alanda yararlı olacağı aynı zamanda bu materyallerin önemarz ettiği kanısına varılmıştır. 

Inthis paper, the biofilm formation of industrial yeasts Candida intermedia NRRL Y-8278 and Kluyveromces marxianus NRRL Y-8281 yeast were grownon various plastic composite supports (PCS) to analyze colony forming unit(CFU). The biofilm was performed using Stripping-Sand method for yeasts on the PCS(PCS1, PCS2, PCS3, PCS4). Thebiofilm formation on the yeasts was observed on the PCS. K. marxianus and C.intermedia took place the apparent the biofilm population CFU of each yeast on plastic supports. The bestbiofilm population was performed 2.9×108CFU g-1 by K. marxianus on PCS2 support than C. intermedia. The calculation ofbiofilm in   CFU mL-1 oneach yeast also was analyzed 1.6×1010by K. marxianus on the PCS2. K. marxianus had better the biofilm values on the PCS than C.intermedia. The results of this work will be useful on the some areas thatthis supports (polypropylene) used in such as food, biomedical and industrialcompanies and to realize about structure of yeasts and polypropylene supportsin the literatures.

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