Extraction, characterization, and evaluation of the functionality of fixed oil low-quality coffee beans for use as pharmaceutical ingredients

Year 2022, Volume: 2 Issue: 2, 155 - 165, 15.12.2022

Ester Moulin ítalo Werner Jaqueline Souza Milene Fontes Janaína Villanova Tercio Souza

Abstract

In order to offer a viable destination for green coffee beans classified as non-beverage type, this work aimed to extract and characterize the fixed oil from these beans and perform a preliminary evaluation of its functionality as a pharmaceutical ingredient. The extraction yield obtained was 3.70 ± 1.29% (w/w). The oil present in its composition high levels of fatty acids with emulsifying and emollient properties, palmitic acid (47.76%) and linoleic acid (32.98%); and compounds with antioxidant functional properties, tocopherols (788.71 ± 56.08 mg/kg) and phenolic compounds (3312.40 ± 14.62 mg/kg). This oil showed antioxidant activity against the free radical 2,2-diphenyl-1-picryl hydrazil at all tested concentrations, reaching 50% inhibition at the concentration of 0.59 mg/ml and 90% at 0.96 mg/ml. The preliminary evaluation of the physical stability of the creams showed that, when incorporated into formulations, this oil has the potential to be used as a substitute for the synthetic ingredients liquid petrolatum, decyl oleate, and butylated hydroxytoluene.

Keywords

Natural antioxidant, Pharmaceutical emulsions, Cosmetics, Green coffee

References

• Abdullah, M., Koc, A.B., 2013. Oil removal from waste coffee grounds using two-phase solvent extraction enhanced with ultrasonication. Renewable Energy, 50, 965-970.
• Acevedo, F., Rubilar, M., Scheuermann, E., Cancino, B., Uquiche, E., Garcés, M., Shene, C., 2013. Spent coffee grounds as a renewable source of bioactive compounds. Journal of Biobased Materials and Bioenergy, 7(3), 420-428.
• Aguiar, A.T.D.E., Salva, T.D.J.G., Fazuoli, L.C., Favarin, J.L., 2005. Variação no teor de lipídios em grãos de variedades de Coffea canephora. Pesquisa Agropecuária Brasileira, 40, 1251-1254.
• Ahmad, A., Ahsan, H., 2020. Lipid-based formulations in cosmeceuticals and biopharmaceuticals. Biomedical Dermatology, 4(1), 1-10.
• Al-Hamamre, Z., Foerster, S., Hartmann, F., Kröger, M., Kaltschmitt, M., 2012. Oil extracted from spent coffee grounds as a renewable source for fatty acid methyl ester manufacturing. Fuel, 96, 70-76.
• Allen Jr, L.V., Popovich, N.G., Ansel, H.C., 2013. Formas Farmacêuticas e Sistemas de Liberação de Fármacos, nine ed. Artmed Editora, Porto Alegre. ISBN 9788565852845.
• Attwood, D., Florence, A.T., Rothschild, Z., 2011. Princípios Físico-Químicos em Farmácia. Second ed. Farmabooks, São Paulo. ISBN 978858973135-5.
• Aulton, M.E, Taylor, K.M.G., 2016. Delineamento de Formas Farmacêuticas, fourth ed, Elsevier, Brasil. ISBN.8535262784, 9788535262780.
• Bera, D., Lahiri, D., Nag, A., 2006. Studies on a natural antioxidant for stabilization of edible oil and comparison with synthetic antioxidants. Journal of Food Engineering, 74(4), 542-545.
• Blasi, F., Cossignani, L., 2020. An overview of natural extracts with antioxidant activity for the improvement of the oxidative stability and shelf life of edible oils. Processes, 8(8), 956.
• Brand-Williams, W., Cuvelier, M.E., Berset, C.L.W.T., 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30.
• Brasil, Anvisa, 2004. Agência Nacional de Vigilância Sanitária. Guia de estabilidade de produtos cosméticos. Editora Anvisa, 2004. ISBN 85-88233-15-0.
• Brasil, Anvisa, 2005. Resolução RDC ANVISA/MS n°.270, de 22 de setembro de 2005. Regulamento Técnico para Óleos Vegetais, Gorduras Vegetais e Creme Vegetal.
• Brasil, Anvisa., 2012. Formulário Nacional da Farmacopeia Brasileira. Edn 2, Agência Nacional de Vigilância Sanitária, Brasília. p. 224.
• Brasil, Companhia Nacional de Abastecimento, 2020a. Acompanhamento da safra brasileira de café, v. 6 – Safra 2020, n. 4 - Quarto levantamento, Brasília, p. 1-45, dezembro 2020. ISSN 2318-7913.
• Brasil, Ministério da Agricultura, Pecuária e Abastecimento, 2003. Instrução Normativa nº 8, de 11 de junho de 2003. Regulamento Técnico de Identidade e de Qualidade para a Classificação do Café Beneficiado Grão Cru. Diário Oficial da República Federativa do Brasil, Brasília, DF, 13 jun. 2003. Seção 1, p. 22-29.
• Brasil, Ministério da Agricultura, Pecuária e Abastecimento, 2020b. Instrução Normativa nº 8, de 11 de junho de 2003. Regulamento Técnico de Identidade e de Qualidade para a Classificação do Café Beneficiado Grão Cru. Diário Oficial da República Federativa do Brasil. Brasília, DF, 15 set. 2020. Seção 1, p. 22-29.
• Brige, F.A.A., 2016. Variabilidade genética e caracterização química de acessos de café conilon em sistema de cultivo irrigado no Cerrado. Disserrtaçao de Mestrado em Agronomia, Universidade de Brasilia.
• Bruxel, F., Laux, M., Wild, L.B., Fraga, M., Koester, L.S., Teixeira, H.F., 2012. Nanoemulsões como sistemas de liberação parenteral de fármacos. Química Nova, 35(9), 1827-1840.
• Cafesul, 2021. Cooperativa dos Cafeicultores do Sul do Estado do Espírito Santo.
• Chiari, B.G., Trovatti, E., Pecoraro, É., Corrêa, M.A., Cicarelli, R.M.B., Ribeiro, S.J.L., Isaac, V.L.B., 2014. Synergistic effect of green coffee oil and synthetic sunscreen for health care application. Industrial Crops and Products, 52, 389-393.
• Cruz, J.M., Conde, E., Domínguez, H., Parajó, J.C., 2007. Thermal stability of antioxidants obtained from wood and industrial wastes. Food Chemistry, 100(3), 1059-1064.
• Dak, M., Verma, R.C., Jaaffrey, S.N.A., 2007. Effect of temperature and concentration on rheological properties of “Kesar” mango juice. Journal of Food Engineering, 80(4), 1011-1015.
• D'Amelio, N., De Angelis, E., Navarini, L., Schievano, E., Mammi, S., 2013. Green coffee oil analysis by high-resolution nuclear magnetic resonance spectroscopy. Talanta, 110, 118-127.
• Dantas, M.B., Albuquerque, A.R., Barros, A.K., Rodrigues Filho, M.G., Antoniosi Filho, N.R., Sinfrônio, F.S.M., Souza, A.G., 2011. Evaluation of the oxidative stability of corn biodiesel. Fuel, 90(2), 773-778.
• de Almeida, L.F., Spers, E.E., 2020. Coffee Consumption and Industry Strategies in Brazil. Woodhead Publishing. ISBN: 978-0-12-814721-4.
• de Lima Souza, J.R.C., Villanova, J.C.O., de Souza, T.D.S., Maximino, R.C., Menini, L., 2021. Vegetable fixed oils obtained from soursop agro-industrial waste: Extraction, characterization and preliminary evaluation of the functionality as pharmaceutical ingredients. Environmental Technology & Innovation, 21, 101379.
• de Oliveira, P.M.A., de Almeida, R.H., de Oliveira, N.A., Bostyn, S., Goncalves, C.B., de Oliveira, A.L., 2014. Enrichment of diterpenes in green coffee oil using supercritical fluid extraction–Characterization and comparison with green coffee oil from pressing. The Journal of Supercritical Fluids, 95, 137-145.
• do Rosário, M.S., Gauto, M.I.R., Silva, A.C.L.N., Sales, J.S., dos Santos Pereira, F., dos Santos, E.P., Costa, M.C.P., 2021. Estudo de estabilidade de emulsão cosmética com potencial de creme hidratante para o tratamento da xerose cutânea utilizando o óleo de babaçu (Orbignya phalerata Martius). Brazilian Journal of Development, 7(3), 29552-29570.
• Durán, C.A., Regalado, E., Guzmán, M., Navas, P.B., 2004. Determinación de vitamina E en aceites. Una experiencia didáctica en Química Analítica Instrumental. Educación Química, 15(4e), 343-348.
• Durán, C.A., Tsukui, A., Santos, F.K.F., Martinez, S.T., Bizzo, H.R., Rezende, C.M., 2017. Café: Aspectos Gerais e seu Aproveitamento para além da Bebida. Revista Virtual de Química, 9(1), 107-134.
• Esquivel Rodríguez, P., Viñas Meneses, M., Steingass, C.B., Gruschwitz, M., Guevara Berger, E., Carle, R., Schweiggert, R.M., Jiménez García, V., 2020. V. Coffee (Coffea arabica L.) by-Products as a Source of Carotenoids and Phenolic Compounds—Evaluation of Varieties with Different Peel Color.
• Esquivel, P., Jimenez, V.M., 2012. Functional properties of coffee and coffee by-products. Food Research International, 46(2), 488-495.
• FAO (Food and Agriculture Organization of the United Nations), 2021. Codex Standards for Fats and Oils from Vegetable Sources. Accessed August 2021.
• Ferrari, R.A., Souza, W.L.D., 2009. Avaliação da estabilidade oxidativa de biodiesel de óleo de girassol com antioxidantes. Química Nova, 32(1), 106-111.
• Fonseca-Santos, B., Corrêa, M.A., Chorilli, M., 2015. Sustainability, natural and organic cosmetics: consumer, products, efficacy, toxicological and regulatory considerations. Brazilian Journal of Pharmaceutical Sciences, 51, 17-26.
• Frelichowska, J., Bolzinger, M.A., Valour, J.P., Mouaziz, H., Pelletier, J., Chevalier, Y., 2009. Pickering w/o emulsions: drug release and topical delivery. International Journal of Pharmaceutics, 368(1-2), 7-15.
• Garg, S.K., Shukla, A., Choudhury, S., 2021. Green coffee beans. In Nutraceuticals Academic Press, Cambridge, pp. 725-748.
• Górnaś, P., Dwiecki, K., Siger, A., Tomaszewska-Gras, J., Michalak, M., Polewski, K., 2016. Contribution of phenolic acids isolated from green and roasted boiled-type coffee brews to total coffee antioxidant capacity. European Food Research and Technology, 242(5), 641-653.
• Górnaś, P., Siger, A., Pugajeva, I., Czubinski, J., Waśkiewicz, A., Polewski, K., 2014. New insights regarding tocopherols in Arabica and Robusta species coffee beans: RP-UPLC-ESI/MSn and NP-HPLC/FLD study. Journal of Food Composition and Analysis, 36(1-2), 117-123.
• Hameed A., Hussain S.A., Suleria H.A.R., 2018. “Coffee Bean-Related” Agroecological Factors Affecting the Coffee. In: Merillon JM., Ramawat K. (eds) Co-Evolution of Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham.
• Hosseini, H., Ghorbani, M., Meshginfar, N., Mahoonak, A.S., 2016. A review on frying: procedure, fat, deterioration progress and health hazards. Journal of the American Oil Chemists' Society, 93(4), 445-466.
• Hu, Y.T., Ting, Y., Hu, J.Y., Hsieh, S.C., 2017. Techniques and methods to study functional characteristics of emulsion systems. Journal of Food and Drug Analysis, 25(1), 16-26.
• Instituto Adolfo Lutz (São Paulo), 2008. Métodos físico-químicos para análise de alimentos: /coordenadores Odair Zenebon, Neus Sadocco Pascuet e Paulo Tiglea -- São Paulo: Instituto Adolfo Lutz. versão eletrônica, 1020p.
• Jenkins, R.W., Ellis, E.H., Lewis, E.J., Paterson, M., Le, C.D., Ting, V.P., Chuck, C.J., 2017. Production of biodiesel from Vietnamese waste coffee beans: biofuel yield, saturation and stability are all elevated compared with conventional coffee biodiesel. Waste and Biomass Valorization, 8(4), 1237-1245.
• Jham, G.N., Winkler, J.K., Berhow, M.A., Vaughn, S.F., 2007. γ-Tocopherol as a marker of Brazilian coffee (Coffea arabica L.) adulteration by corn. Journal of Agricultural and Food Chemistry, 55(15), 5995-5999.
• Kalschne, D.L., Viegas, M.C., De Conti, A.J., Corso, M.P., de Toledo Benassi, M., 2018. Steam pressure treatment of defective Coffea canephora beans improves the volatile profile and sensory acceptance of roasted coffee blends. Food Research International, 105, 393-402.
• Keen, M.A., Hassan, I., 2016. Vitamin E in dermatology. Indian Dermatology Online Journal, 7(4), 311.
• Kemsley, E.K., Ruault, S., Wilson, R.H., 1995. Discrimination between Coffea arabica and Coffea canephora variant robusta beans using infrared spectroscopy. Food Chemistry, 54(3), 321-326.
• López-Barrera, D.M., Vázquez-Sánchez, K., Loarca-Piña, M.G.F., Campos-Vega, R., 2016. Spent coffee grounds, an innovative source of colonic fermentable compounds, inhibit inflammatory mediators in vitro. Food Chemistry, 212, 282-290.
• Lourenço, S.C., Moldão-Martins, M., Alves, V.D., 2019. Antioxidants of natural plant origins: From sources to food industry applications. Molecules, 24(22), 4132.
• Lu, C., Napier, J.A., Clemente, T.E., Cahoon, E.B., 2011. New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications. Current Opinion in Biotechnology, 22(2), 252-259.
• Mazzafera, P., Soave, D., Zullo, M.A.T., Guerreiro Filho, O., 1998. Oil content of green beans from some coffee species. Bragantia, 57(1), 45-48.
• Mezger, T.G., 2020. The rheology handbook: for users of rotational and oscillatory rheometers. Fifth edition, European Coatings, Hanover. ISBN 978-3-86630-532-8.
• Milatovic, D., Zaja-Milatovic, S., Gupta, R.C., 2016. Oxidative stress and excitotoxicity: antioxidants from nutraceuticals. In Nutraceuticals. Academic Press, Cambridge. 401-413.
• Mohsin, S., Akhtar, N., Mahmood, T., Khan, H., Mustafa, R., 2016. Formulation and stability of topical water in oil emulsion containing corn silk extract. Tropical Journal of Pharmaceutical Research, 15(6), 1115-1121.
• Montenegro, L., Rapisarda, L., Ministeri, C., Puglisi, G., 2015. Effects of lipids and emulsifiers on the physicochemical and sensory properties of cosmetic emulsions containing vitamin E. Cosmetics, 2(1), 35-47.
• Ndayishimiye, J., Chun, B.S., 2017. Optimization of carotenoids and antioxidant activity of oils obtained from a co-extraction of citrus (Yuzu ichandrin) by-products using supercritical carbon dioxide. Biomass and Bioenergy, 106, 1-7.
• Nosari, A.B., Lima, J.F., Serra, O.A., Freitas, L.A.P., 2015. Improved green coffee oil antioxidant activity for cosmetical purpose by spray drying microencapsulation. Revista Brasileira de Farmacognosia, 25, 307-311.
• Oliveira, É.R., Carvalho, G.R., Cirillo, M.Â., Queiroz, F., 2020. Effect of ecofriendly bio-based solvents on oil extraction from green coffee bean and its industrial press cake. Brazilian Journal of Chemical Engineering, 36(4), 1739-1753.
• Otemuyiwa, I.O., Adewusi, S.R., 2013. Fatty acid, carotenoid and tocopherol content of some fast foods from a Nigerian eatery. Journal of Food and Nutrition Research, 1(5), 82-86.
• Parente, M.E., Ga´ Mbaro, A., Solana, G., 2005. Study of sensory properties of emollients used in cosmetics and their correlation with physicochemical properties. International Journal of Cosmetic Science, 27(6), 354-354.
• Pereda, M.D.V., Dieamant, G., Eberlin, S., Nogueira, C., Colombi, Stasi, L.C.D., Queiroz, M.L.S., 2009. Effect of green Coffea arabica L. seed oil on extracellular matrix components and water‐channel expression in in vitro and ex vivo human skin models. Journal of Cosmetic Dermatology, 8(1), 56-62.
• Pereira, N.P., Miguel, O.G., Miguel, M.D., 2005. Chemical composition of the fixed oil from [Chamomilla recutita (L.) Rauschert] dried fruits native to the municipality of Mandirituba, Paraná (PR). Revista Brasileira de Farmacognosia, 15, 334-337.
• Poljšak, N., Kreft, S., Kočevar Glavač, N., 2020. Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology. Phytotherapy Research, 34(2), 254-269.
• Poltronieri, P., Rossi, F., 2016. Challenges in specialty coffee processing and quality assurance. Challenges, 7(2), 19.
• Proksch, E., 2018. pH in nature, humans and skin. The Journal of Dermatology, 45(9), 1044-1052.
• R: Development Core Team, 2019. A Language and Environment for Statistical Computing. Version 3.6.2 (2019-12-12). The R Foundation for Statistical Computing.
• Rabinovich-Guilatt, L., Dubernet, C., Gaudin, K., Lambert, G., Couvreur, P., Chaminade, P., 2005. Phospholipid hydrolysis in a pharmaceutical emulsion assessed by physicochemical parameters and a new analytical method. European Journal of Pharmaceutics and Biopharmaceutics, 61(1-2), 69-76.
• Ren, Y., Wang, C., Xu, J., Wang, S., 2019. Cafestol and kahweol: A review on their bioactivities and pharmacological properties. International Journal of Molecular Sciences, 20(17), 4238.
• Rodrigues, N.P., Bragagnolo, N., 2013. Identification and quantification of bioactive compounds in coffee brews by HPLC–DAD–MSn. Journal of Food Composition and Analysis, 32(2), 105-115.
• Rosiaux, Y., Jannin, V., Hughes, S., Marchaud, D., 2015. Solid lipid excipients as matrix agents for sustained drug delivery. Excipient Applications in Formulation Design and Drug Delivery, 237-271.
• Rowe, R.C., Sheskey, P., Quinn, M., 2009. Handbook of pharmaceutical excipients. Libros Digitales. Sixth ed. Pharmaceutical Press, Washington. ISBN 978 1 58212 135 2. Diponível em:
• Salmela, L., Washington, C., 2014. A continuous flow method for estimation of drug release rates from emulsion formulations. International Journal of Pharmaceutics, 472(1-2), 276-281.
• Santos, F.L., Nantes, J.F.D., 2014. Coordenação no mercado do café brasileiro: o desserviço da classificação por defeitos. Gestão & Produção, 21, 586-599.
• Sarkar, R., Podder, I., Gokhale, N., Jagadeesan, S., Garg, V.K., 2017. Use of vegetable oils in dermatology: an overview. International Journal of Dermatology, 56(11), 1080-1086.
• Scott, S.E., Rozin, P., Small, D.A., 2020. Consumers prefer “natural” more for preventatives than for curatives. Journal of Consumer Research, 47(3), 454-471.
• Shamsudin, R., Ling, C.S., Adzahan, N.M., Daud, W.R.W., 2013. Rheological properties of ultraviolet-irradiated and thermally pasteurized Yankee pineapple juice. Journal of Food Engineering, 116(2), 548-553.
• Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M., 1999. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in enzymology (Vol. 299, pp. 152-178). Academic press.
• Somnuk, K., Eawlex, P., Prateepchaikul, G., 2017. Optimization of coffee oil extraction from spent coffee grounds using four solvents and prototype-scale extraction using circulation process. Agriculture and Natural Resources, 51(3), 181-189.
• Speer, K., Kölling-Speer, I., 2006. The lipid fraction of the coffee bean. Brazilian Journal of Plant Physiology, 18(1), 201-216.
• Spernath, A., Aserin, A., Sintov, A.C., Garti, N., 2008. Phosphatidylcholine embedded micellar systems: Enhanced permeability through rat skin. Journal of Colloid and Interface Science, 318(2), 421-429.
• Taghvaei, M., Jafari, S.M., 2015. Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. Journal of Food Science and Technology, 52(3), 1272-1282.
• Toci, A.T., Farah, A., 2014. Volatile fingerprint of Brazilian defective coffee seeds: Corroboration of potential marker compounds and identification of new low quality indicators. Food Chemistry, 153, 298-314.
• Tsukui, A., Júnior, H.S., Oigman, S.S., De Souza, R.O.M.A., Bizzo, H.R., Rezende, C.M., 2014. Microwave-assisted extraction of green coffee oil and quantification of diterpenes by HPLC. Food Chemistry, 164, 266-271.
• Wagemaker, T.A.L., Carvalho, C.R.L., Maia, N.B., Baggio, S.R., Guerreiro Filho, O., 2011. Sun protection factor, content and composition of lipid fraction of green coffee beans. Industrial Crops and Products, 33(2), 469-473.
• Wagemaker, T.A.L., Fernandes, A.S., Campos, P.M., Rodrigues, L.M., Rijo, P., 2012. Evaluation of antioxidant and antimicrobial activities of green coffee oil in cosmetic formulations. Biomedical and Biopharmaceutical Research, 2(9), 207-214.
• Wagemaker, T.A.L., Fernandes, A.S., Rosado, C., Rijo, P., Campos, P.M., Rodrigues, L.M., 2013. Safety of green coffee oil in cosmetic formulations: from in vitro to clinical studies. Toxicology Letters, (221), S231.
• Wagemaker, T.A.L., Silva, S.A., Leonardi, G.R., Campos, P.M.M., 2015. Green Coffea arabica L. seed oil influences the stability and protective effects of topical formulations. Industrial Crops and Products, 63, 34-40.
• Wei, F., Tanokura, M., 2015. Organic compounds in green coffee beans. In Coffee in health and disease prevention. Academic Press, Cambridge, pp. 149-162.
• Xu, H., Wang, W., Liu, X., Yuan, F., Gao, Y., 2015. Antioxidative phenolics obtained from spent coffee grounds (Coffea arabica L.) by subcritical water extraction. Industrial Crops and Products, 76, 946-954.
• Zielińska, A., Nowak, I., 2017. Abundance of active ingredients in sea-buckthorn oil. Lipids in Health and Disease, 16(1), 1-11.