Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates

Year 2019, Volume: 1 Issue: 1, 30 - 41, 19.08.2019

Tuba Yıldırım , Farid Sadati Berna Kocaman , Belgin Siriken

https://doi.org/10.38058/ijsl.596007

Cited By: 7

Abstract

In
the present study, a total 110 (60 cheese and 50 cows’ raw milk) samples was analyzed
for CPS according to Food Drug Administration (FDA, 2001) previously. After the
isolation, to confirmation of the isolates, catalase test, microscopic
examination, coagulase test in tube and glucose-mannitol fermentation tests
were applied. After the tests, we obtained 97 CPS isolates. The 97 CPS isolates
were used as a material. For the confirmation of the isolates being Staphylococci species, 16S rRNA was
detected by using PCR assay. For the detection of the CPS isolates being S. aureus, nuc gene detected in the CPS isolates using PCR assay. To
determination of the staphylococcal enterotoxin, PCR assay was applied. The 16 S rRNA was detected in a total 97
(35 milk origin and 62 cheese origin) isolates. Therefore, these isolates were
evaluated as coagulase-positive
staphylococci (CPS).  The nuc gene was detected in 50 out of 97
CPS isolates. So, the 50 (18 cheese origin and 32 milk origin) isolates were
evaluated as S. aureus. However, none of the staphylococcal enterotoxin
genes

(SE A,B,C,D,E,G,H,I,J)
was detected in 97 CPS or 50 S. aureus isolates.

Keywords

Food Contamination, Bacterial Toxin, CPS, nuc Gene

Supporting Institution

Amasya University

Project Number

FMB-BAP-12-012

Thanks

This study is thankful to Amasya University (FMB-BAP-12-012) for financial support.

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