int. j. sec. metabolite International Journal of Secondary Metabolite 2148-6905 İzzet KARA 10.21448/ijsm.356247 Structural Biology Yapısal Biyoloji Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum Karan Tunay Erenler Ramazan 11 25 2017 4 3, Special Issue 1 16 19 11 19 2017 07 15 2017 Copyright © 2014, International Journal of Secondary Metabolite 2014 International Journal of Secondary Metabolite

Cyanobacterial metabolites are natural products that have an important feature in pharmaceutical and medicinal industries. In this study, the presence of the secondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler) Anagnostidis isolated from Tokat Yesilirmak River and its production in salt stress and pH stress was investigated. In salt stress experiments, cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium in erlenmayers of 0.5, 1.0, 3.0, 5.0 M. pH stress was executed at 5 and 9. Norharmane amount was determined by HPLC using C18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml / min. The amount of norharman metabolite (μg/g) was calculated according to the Gauss method by drawing a calibration curve over the absorbance value of the standard 247 nm wavelength. According to the analysis results, metabolite production was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M, there was no norharmane production. The norharmane production is higher at pH 5 (1.293 μg /g) than that of the pH 9 (0.448 μg /g).

Cyanobacterialmetabolites are natural products that have an important feature inpharmaceutical and medicinal industries. In this study, the presence of thesecondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler)Anagnostidis isolated from Tokat Yesilirmak River and its production in saltstress and pH stress was investigated. In salt stress experiments,cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium inerlenmayers of 0.5, 1.0, 3.0, 5.0 M. pH stresswas executed at 5 and 9. Norharmane amount was determined by HPLC usingC18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml /min. The amount of norharman metabolite (μg/g) was calculated according to theGauss method by drawing a calibration curve over the absorbance value of thestandard 247 nm wavelength. According to the analysis results, metaboliteproduction was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M,there was no norharmane production. Thenorharmane production is higher at pH 5 (1.293 μg /g) than that of the pH 9 (0.448 μg /g).

 Geitlerinema carotinosum stress conditions norharmane HPLC Geitlerinema carotinosum stress conditions norharmane HPLC Sinha R.P., Häder D.-P. (2008) UV-protectants in cyanobacteria, Plant Science, 174(3), 278-89. Burja A.M., Banaigs B., Abou-Mansour E., Burgess J.G., Wright P.C. (2001) Marine cyanobacteria-a prolific source of natural products, Tetrahedron, 57(46), 9347-77. Pandey U., Pandey J. (2008) Enhanced production of biomass, pigments and antioxidant capacity of a nutritionally important cyanobacterium Nostochopsis lobatus, Bioresource technology, 99(10), 4520-23. Kodani S., Imoto A., Mitsutani A., Murakami M. (2002) Isolation and identification of the antialgal compound, harmane (1-methyl-β-carboline), produced by the algicidal bacterium, Pseudomonas sp. K44-1, Journal of applied phycology, 14(2), 109-14. Galhano V., Santos H., Oliveira M.M., Gomes-Laranjo J., Peixoto F. (2011) Changes in fatty acid profile and antioxidant systems in a Nostoc muscorum strain exposed to the herbicide bentazon, Process Biochemistry, 46(11), 2152-62. Pelah D., Sintov A., Cohen E. (2004) The effect of salt stress on the production of canthaxanthin and astaxanthin by Chlorella zofingiensis grown under limited light intensity, World Journal of Microbiology and Biotechnology, 20(5), 483-86. Sivonen K. (1990) Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains, Applied and Environmental Microbiology, 56(9), 2658-66. Erenler R., Telci I., Ulutas M., Demirtas I., Gul F., Elmastas M., et al. (2015) Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt, Journal of Food Biochemistry, 39(5), 622-30. John D.M., Whitton B.A., Brook A.J. The Freshwater Algal Flora of the British Isles, An Identification Guide to Freshwater and Terrestrial Algae. UK, Cambridge University Press; 2002. Karan T., Dastan T., Baral I., Altuner Z. (2016) Effects of Differential Time Applications on Some Cyanobacterial Norharman Production Rates, Cumhuriyet University Faculty of Science, 37(4), 398-404. Hagemann M. (2011) Molecular biology of cyanobacterial salt acclimation, FEMS microbiology reviews, 35(1), 87-123. Gupta N., Bhaskar A., Rao P.L. (2002) Growth characteristics and toxin production in batch cultures of Anabaena flos-aquae: effects of culture media and duration, World Journal of Microbiology and Biotechnology, 18(1), 29-35. Wagacha J., Duboise S., Amugune N., Muruga B., Kabaru J. (2014) Effect of physicochemical conditions on growth rates of cyanobacteria species isolated from Lake Magadi, a soda lake in Kenya, WebPub Journal of Scientific Research Vol. 2(5), pp. 41-50. Karan T., Koyuncu M., Parmaksiz I., Pabuccu K., Altuner Z. (2015) Siyanotoksinler ve Biyosentezlerinden Sorumlu Genlerin Düzenlenmesi, Gaziosmanpasa Bilimsel Arastirma Dergisi, 9(11), 54-69. Gupta S., Agrawal S. (2006) Survival of blue-green and green algae under stress conditions, Folia microbiologica, 51(2), 121-28.