Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic
to Central Asia, is a valuable saponin-bearing technical and medicinal plant.
The purpose of this study was to develop in
vitro mass propagation for the conservation of endangered species. Node
explants were induced to regenerate adventitious shoot buds on Murashige and
Skoog medium (MS) supplemented with different concentrations of
6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid
(NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was
recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month
cultivation. For elongation, obtained shoot conglomerates were transfered for
hormone-free MS. The cultivation of initial explants on MS with kinetin led to
a three-fold reduction in the number of shoots characterized by a maximum
length and clearly defined internodes (without the stage of elongation).
Micropropagation was achieved by cutting obtained shoots and adventitious shoot
induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0
mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to
elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In
both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60
%, respectively) at 20-day’s incubation. Type of auxin was influenced most on
root quality (thickness, color, branching) than on their frequency and number.
Allochrusa gypsophiloides, Turkestan soaproot, Saponin-bearing plant, Adventitious shoot, Micropropagation