Year 2020, Volume 7 , Issue 1, Pages 1 - 7 2020-03-20

Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Valentina MURSALIYEVA [1] , Akjunus IMANBAYEVA [2]


Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number.
Allochrusa gypsophiloides, Turkestan soaproot, Saponin-bearing plant, Adventitious shoot, Micropropagation
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Primary Language en
Subjects Biology
Published Date March
Journal Section Articles
Authors

Orcid: 0000-0003-4803-9169
Author: Valentina MURSALIYEVA (Primary Author)
Institution: Institute of Plant Biology and Biotechnology
Country: Kazakhstan


Author: Akjunus IMANBAYEVA
Institution: Mangyshlak Experimental Botanical Garden
Country: Kazakhstan


Supporting Institution Ministry of Education and Science of Republic of Kazakhstan
Project Number BRO5236506
Dates

Publication Date : March 20, 2020

Bibtex @research article { ijsm627140, journal = {International Journal of Secondary Metabolite}, issn = {}, eissn = {2148-6905}, address = {Prof. Dr. İzzet KARA}, publisher = {İzzet KARA}, year = {2020}, volume = {7}, pages = {1 - 7}, doi = {10.21448/ijsm.627140}, title = {Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins}, key = {cite}, author = {MURSALIYEVA, Valentina and IMANBAYEVA, Akjunus} }
APA MURSALIYEVA, V , IMANBAYEVA, A . (2020). Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins. International Journal of Secondary Metabolite , 7 (1) , 1-7 . DOI: 10.21448/ijsm.627140
MLA MURSALIYEVA, V , IMANBAYEVA, A . "Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins". International Journal of Secondary Metabolite 7 (2020 ): 1-7 <https://dergipark.org.tr/en/pub/ijsm/issue/52291/627140>
Chicago MURSALIYEVA, V , IMANBAYEVA, A . "Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins". International Journal of Secondary Metabolite 7 (2020 ): 1-7
RIS TY - JOUR T1 - Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins AU - Valentina MURSALIYEVA , Akjunus IMANBAYEVA Y1 - 2020 PY - 2020 N1 - doi: 10.21448/ijsm.627140 DO - 10.21448/ijsm.627140 T2 - International Journal of Secondary Metabolite JF - Journal JO - JOR SP - 1 EP - 7 VL - 7 IS - 1 SN - -2148-6905 M3 - doi: 10.21448/ijsm.627140 UR - https://doi.org/10.21448/ijsm.627140 Y2 - 2019 ER -
EndNote %0 International Journal of Secondary Metabolite Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins %A Valentina MURSALIYEVA , Akjunus IMANBAYEVA %T Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins %D 2020 %J International Journal of Secondary Metabolite %P -2148-6905 %V 7 %N 1 %R doi: 10.21448/ijsm.627140 %U 10.21448/ijsm.627140
ISNAD MURSALIYEVA, Valentina , IMANBAYEVA, Akjunus . "Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins". International Journal of Secondary Metabolite 7 / 1 (March 2020): 1-7 . https://doi.org/10.21448/ijsm.627140
AMA MURSALIYEVA V , IMANBAYEVA A . Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins. Int. J. Sec. Metabolite. 2020; 7(1): 1-7.
Vancouver MURSALIYEVA V , IMANBAYEVA A . Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins. International Journal of Secondary Metabolite. 2020; 7(1): 7-1.