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Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Year 2020, Volume: 7 Issue: 1, 1 - 7, 20.03.2020
https://doi.org/10.21448/ijsm.627140

Abstract

Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic
to Central Asia, is a valuable saponin-bearing technical and medicinal plant.
The purpose of this study was to develop in
vitro
mass propagation for the conservation of endangered species. Node
explants were induced to regenerate adventitious shoot buds on Murashige and
Skoog medium (MS) supplemented with different concentrations of
6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid
(NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was
recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month
cultivation. For elongation, obtained shoot conglomerates were transfered for
hormone-free MS. The cultivation of initial explants on MS with kinetin led to
a three-fold reduction in the number of shoots characterized by a maximum
length and clearly defined internodes (without the stage of elongation).
Micropropagation was achieved by cutting obtained shoots and adventitious shoot
induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0
mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to
elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In
both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60
%, respectively) at 20-day’s incubation. Type of auxin was influenced most on
root quality (thickness, color, branching) than on their frequency and number.

Supporting Institution

Ministry of Education and Science of Republic of Kazakhstan

Project Number

BRO5236506

References

  • Eisenman, S., Strume, L., Zaurov, D. (2012). Medical plants of Central Asia: Uzbekistan and Kyrgyzstan; Springer; New York, USA. ISBN 13: 9781461439110.
  • Bespayev, S. (1966). Acanthophyllum gypsophiloides in Kazakhstan: morphology, taxonomy, phytosociology, testing in culture. Thesis on scient. degree of cand. biol. sci., Kazakh State University, Alma-Ata, 09.06.1966.
  • Red Data Book of the Kazakhstan (2014). 2rd ed; АrtPrintXXI, Astana, Kazakhstan, 2: 60.
  • Kondratenko, E., Putieva, Z., Abubakirov, N. (1981). Triterpenoid glycosides of plants of the family Caryophyllaceae. Chem. Nat. Comp., 17, 303-317. doi: 10.3762/bjoc.8.87
  • Battger, S., Melzig, M. (2001). Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem. Lett, 4 (2):59-68. doi: 10.1016/j.phytol.2010.08.003
  • Mursaliyeva, V., Kozhebayeva, Zh., Rakhimbayev, I., Gemejiyeva, N. (2016). Qualitative and quantitative analysis of saponins in Allochrusa gypsophiloides. KazNU Bulletin. Biol. series, 3(68), 115-123.
  • Grudzinskaya, L., Gemedzhieva, N. (2012). List of medicinal plants in Kazakhstan. Almaty, Kazakhstan. 55. ISBN: 978-601-80248-6-3.
  • Tumagambetova, A., Alexyuk, P., Bogoyavlenskij, V., Zaitseva, E., Omirtaeva, M., Alexyur, M., Sokolova, N. (2017). Adjuvant activity of saponins from Kazakhstani plants on the immune response to subunit influenza vaccine. Archives of Virology, 162 (12), 3817-3826.
  • Aleksyuk, P., Moldakhanov, E., Akanov, K., Anarkulova, E., Bogoiavlenskii, А. (2014). Standardization of saponin’s drugs with antiviral activity. Inter. J. Appl. & Fundam. Res., 6, 80-81.
  • Man, S., Gao, W., Zhang, Y., Huang, L., Liu, C. (2010). Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia, 81(7), 703-714. doi: 10.1016/j.fitote.2010.06.004
  • Gemejiyeva, N., Mursaliyeva, V., Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk. natural populations in the South-Kazakhstan region. News of Nation. Acad. Sci. Rep. of Kazakhstan. Ser. Biol. and Medic., 1(313), 22-29. ISSN 2224-5308.
  • Barakat, M., Heba, E. (2011). In vitro culture and plant regeneration from shoot tip and lateral bud explant of Gypsophila paniculata L. J. Med. Plants Res., 5(15), 3351-3358.
  • Hussain, Md., Fareed, S., Ansari, S., Rahman, Md., Ahmad, L., Saeed, M. (2012). Current approaches toward production of secondary plant metabolites. J. Pharm. Bioall. Sci., 4(1), 10-20.
  • Murashige, T., Skoog, E. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plan., 15, 473-497.

Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Year 2020, Volume: 7 Issue: 1, 1 - 7, 20.03.2020
https://doi.org/10.21448/ijsm.627140

Abstract

Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number.

Project Number

BRO5236506

References

  • Eisenman, S., Strume, L., Zaurov, D. (2012). Medical plants of Central Asia: Uzbekistan and Kyrgyzstan; Springer; New York, USA. ISBN 13: 9781461439110.
  • Bespayev, S. (1966). Acanthophyllum gypsophiloides in Kazakhstan: morphology, taxonomy, phytosociology, testing in culture. Thesis on scient. degree of cand. biol. sci., Kazakh State University, Alma-Ata, 09.06.1966.
  • Red Data Book of the Kazakhstan (2014). 2rd ed; АrtPrintXXI, Astana, Kazakhstan, 2: 60.
  • Kondratenko, E., Putieva, Z., Abubakirov, N. (1981). Triterpenoid glycosides of plants of the family Caryophyllaceae. Chem. Nat. Comp., 17, 303-317. doi: 10.3762/bjoc.8.87
  • Battger, S., Melzig, M. (2001). Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem. Lett, 4 (2):59-68. doi: 10.1016/j.phytol.2010.08.003
  • Mursaliyeva, V., Kozhebayeva, Zh., Rakhimbayev, I., Gemejiyeva, N. (2016). Qualitative and quantitative analysis of saponins in Allochrusa gypsophiloides. KazNU Bulletin. Biol. series, 3(68), 115-123.
  • Grudzinskaya, L., Gemedzhieva, N. (2012). List of medicinal plants in Kazakhstan. Almaty, Kazakhstan. 55. ISBN: 978-601-80248-6-3.
  • Tumagambetova, A., Alexyuk, P., Bogoyavlenskij, V., Zaitseva, E., Omirtaeva, M., Alexyur, M., Sokolova, N. (2017). Adjuvant activity of saponins from Kazakhstani plants on the immune response to subunit influenza vaccine. Archives of Virology, 162 (12), 3817-3826.
  • Aleksyuk, P., Moldakhanov, E., Akanov, K., Anarkulova, E., Bogoiavlenskii, А. (2014). Standardization of saponin’s drugs with antiviral activity. Inter. J. Appl. & Fundam. Res., 6, 80-81.
  • Man, S., Gao, W., Zhang, Y., Huang, L., Liu, C. (2010). Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia, 81(7), 703-714. doi: 10.1016/j.fitote.2010.06.004
  • Gemejiyeva, N., Mursaliyeva, V., Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk. natural populations in the South-Kazakhstan region. News of Nation. Acad. Sci. Rep. of Kazakhstan. Ser. Biol. and Medic., 1(313), 22-29. ISSN 2224-5308.
  • Barakat, M., Heba, E. (2011). In vitro culture and plant regeneration from shoot tip and lateral bud explant of Gypsophila paniculata L. J. Med. Plants Res., 5(15), 3351-3358.
  • Hussain, Md., Fareed, S., Ansari, S., Rahman, Md., Ahmad, L., Saeed, M. (2012). Current approaches toward production of secondary plant metabolites. J. Pharm. Bioall. Sci., 4(1), 10-20.
  • Murashige, T., Skoog, E. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plan., 15, 473-497.
There are 14 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Valentina Mursalıyeva 0000-0003-4803-9169

Akjunus Imanbayeva This is me

Project Number BRO5236506
Publication Date March 20, 2020
Submission Date September 30, 2019
Published in Issue Year 2020 Volume: 7 Issue: 1

Cite

APA Mursalıyeva, V., & Imanbayeva, A. (2020). Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins. International Journal of Secondary Metabolite, 7(1), 1-7. https://doi.org/10.21448/ijsm.627140
International Journal of Secondary Metabolite

e-ISSN: 2148-6905