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Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Year 2020, Volume 7, Issue 1, 1 - 7, 20.03.2020
https://doi.org/10.21448/ijsm.627140

Abstract

Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number.

References

  • Eisenman, S., Strume, L., Zaurov, D. (2012). Medical plants of Central Asia: Uzbekistan and Kyrgyzstan; Springer; New York, USA. ISBN 13: 9781461439110.
  • Bespayev, S. (1966). Acanthophyllum gypsophiloides in Kazakhstan: morphology, taxonomy, phytosociology, testing in culture. Thesis on scient. degree of cand. biol. sci., Kazakh State University, Alma-Ata, 09.06.1966.
  • Red Data Book of the Kazakhstan (2014). 2rd ed; АrtPrintXXI, Astana, Kazakhstan, 2: 60.
  • Kondratenko, E., Putieva, Z., Abubakirov, N. (1981). Triterpenoid glycosides of plants of the family Caryophyllaceae. Chem. Nat. Comp., 17, 303-317. doi: 10.3762/bjoc.8.87
  • Battger, S., Melzig, M. (2001). Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem. Lett, 4 (2):59-68. doi: 10.1016/j.phytol.2010.08.003
  • Mursaliyeva, V., Kozhebayeva, Zh., Rakhimbayev, I., Gemejiyeva, N. (2016). Qualitative and quantitative analysis of saponins in Allochrusa gypsophiloides. KazNU Bulletin. Biol. series, 3(68), 115-123.
  • Grudzinskaya, L., Gemedzhieva, N. (2012). List of medicinal plants in Kazakhstan. Almaty, Kazakhstan. 55. ISBN: 978-601-80248-6-3.
  • Tumagambetova, A., Alexyuk, P., Bogoyavlenskij, V., Zaitseva, E., Omirtaeva, M., Alexyur, M., Sokolova, N. (2017). Adjuvant activity of saponins from Kazakhstani plants on the immune response to subunit influenza vaccine. Archives of Virology, 162 (12), 3817-3826.
  • Aleksyuk, P., Moldakhanov, E., Akanov, K., Anarkulova, E., Bogoiavlenskii, А. (2014). Standardization of saponin’s drugs with antiviral activity. Inter. J. Appl. & Fundam. Res., 6, 80-81.
  • Man, S., Gao, W., Zhang, Y., Huang, L., Liu, C. (2010). Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia, 81(7), 703-714. doi: 10.1016/j.fitote.2010.06.004
  • Gemejiyeva, N., Mursaliyeva, V., Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk. natural populations in the South-Kazakhstan region. News of Nation. Acad. Sci. Rep. of Kazakhstan. Ser. Biol. and Medic., 1(313), 22-29. ISSN 2224-5308.
  • Barakat, M., Heba, E. (2011). In vitro culture and plant regeneration from shoot tip and lateral bud explant of Gypsophila paniculata L. J. Med. Plants Res., 5(15), 3351-3358.
  • Hussain, Md., Fareed, S., Ansari, S., Rahman, Md., Ahmad, L., Saeed, M. (2012). Current approaches toward production of secondary plant metabolites. J. Pharm. Bioall. Sci., 4(1), 10-20.
  • Murashige, T., Skoog, E. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plan., 15, 473-497.

Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins

Year 2020, Volume 7, Issue 1, 1 - 7, 20.03.2020
https://doi.org/10.21448/ijsm.627140

Abstract

Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number.

References

  • Eisenman, S., Strume, L., Zaurov, D. (2012). Medical plants of Central Asia: Uzbekistan and Kyrgyzstan; Springer; New York, USA. ISBN 13: 9781461439110.
  • Bespayev, S. (1966). Acanthophyllum gypsophiloides in Kazakhstan: morphology, taxonomy, phytosociology, testing in culture. Thesis on scient. degree of cand. biol. sci., Kazakh State University, Alma-Ata, 09.06.1966.
  • Red Data Book of the Kazakhstan (2014). 2rd ed; АrtPrintXXI, Astana, Kazakhstan, 2: 60.
  • Kondratenko, E., Putieva, Z., Abubakirov, N. (1981). Triterpenoid glycosides of plants of the family Caryophyllaceae. Chem. Nat. Comp., 17, 303-317. doi: 10.3762/bjoc.8.87
  • Battger, S., Melzig, M. (2001). Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem. Lett, 4 (2):59-68. doi: 10.1016/j.phytol.2010.08.003
  • Mursaliyeva, V., Kozhebayeva, Zh., Rakhimbayev, I., Gemejiyeva, N. (2016). Qualitative and quantitative analysis of saponins in Allochrusa gypsophiloides. KazNU Bulletin. Biol. series, 3(68), 115-123.
  • Grudzinskaya, L., Gemedzhieva, N. (2012). List of medicinal plants in Kazakhstan. Almaty, Kazakhstan. 55. ISBN: 978-601-80248-6-3.
  • Tumagambetova, A., Alexyuk, P., Bogoyavlenskij, V., Zaitseva, E., Omirtaeva, M., Alexyur, M., Sokolova, N. (2017). Adjuvant activity of saponins from Kazakhstani plants on the immune response to subunit influenza vaccine. Archives of Virology, 162 (12), 3817-3826.
  • Aleksyuk, P., Moldakhanov, E., Akanov, K., Anarkulova, E., Bogoiavlenskii, А. (2014). Standardization of saponin’s drugs with antiviral activity. Inter. J. Appl. & Fundam. Res., 6, 80-81.
  • Man, S., Gao, W., Zhang, Y., Huang, L., Liu, C. (2010). Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia, 81(7), 703-714. doi: 10.1016/j.fitote.2010.06.004
  • Gemejiyeva, N., Mursaliyeva, V., Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk. natural populations in the South-Kazakhstan region. News of Nation. Acad. Sci. Rep. of Kazakhstan. Ser. Biol. and Medic., 1(313), 22-29. ISSN 2224-5308.
  • Barakat, M., Heba, E. (2011). In vitro culture and plant regeneration from shoot tip and lateral bud explant of Gypsophila paniculata L. J. Med. Plants Res., 5(15), 3351-3358.
  • Hussain, Md., Fareed, S., Ansari, S., Rahman, Md., Ahmad, L., Saeed, M. (2012). Current approaches toward production of secondary plant metabolites. J. Pharm. Bioall. Sci., 4(1), 10-20.
  • Murashige, T., Skoog, E. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plan., 15, 473-497.

Details

Primary Language English
Subjects Biology
Published Date March
Journal Section Articles
Authors

Valentina MURSALIYEVA> (Primary Author)
Institute of Plant Biology and Biotechnology
0000-0003-4803-9169
Kazakhstan


Akjunus IMANBAYEVA This is me
Mangyshlak Experimental Botanical Garden
Kazakhstan

Supporting Institution Ministry of Education and Science of Republic of Kazakhstan
Project Number BRO5236506
Publication Date March 20, 2020
Published in Issue Year 2020, Volume 7, Issue 1

Cite

APA Mursalıyeva, V. & Imanbayeva, A. (2020). Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins . International Journal of Secondary Metabolite , 7 (1) , 1-7 . DOI: 10.21448/ijsm.627140

International Journal of Secondary Metabolite (IJSM)

ISSN-e: 2148-6905