Characterization of Bioactive and Antioxidant Composition of Mountain Tea (Sideritis montana ssp. montana): Microwave-Assisted Technology

Year 2021, Volume: 8 Issue: 2, 159 - 171, 15.06.2021

Emel Akbaba

https://doi.org/10.21448/ijsm.926926

Cited By: 2

Abstract

The use of synthetic antioxidants has been restricted and even prohibited in some countries due to their toxic effects. This study aims to perform the biochemical assays representing the bioactive compounds in Sideritis montana L. ssp. montana L. known as mountain tea. The study specifically investigates the potential application of mountain tea in the food industry as a natural antioxidant. Different extraction procedures using various solvents are carried out to obtain phenolic compounds and antioxidant activities in plant matrices. In this study, the maceration method was used for the extraction procedure. Furthermore, the microwave-assisted extraction method was applied to shorten the extraction time and decrease the solvent quantity. Methanol was used as a solvent in both conventional and microwave-assisted extraction procedures. The samples were evaluated in terms of the total phenolic compounds, total flavonoids, antioxidant activities, and metal chelating capacity. The obtained results for classical extraction and microwave-assisted extraction methods were found to be 51 and 55 mg/g for TPC, 16.3 and 22 mg/g for TFC, 93 and 103 mg/g for FRAP, and 284 and 282 mg/g for MCC, respectively. The microwave-assisted extraction method produced higher concentrations of bioactive molecules as compared to the classical method. Microwave-assisted extraction was found to be superior to the conventional method in terms of effectiveness, extraction time, and solvent quantity. Besides, Sideritis montana ssp. montana is suggested in the food industry as a natural antioxidant instead of synthetic ones to prevent health-damaging effects.

Keywords

Microwave-assisted extraction, Phenolics, Mountain tea, Antioxidant, Sideritis

References

• Al Jitan, S., Alkhoori, S. A., & Yousef, L. F. (2018). Phenolic Acids From Plants: Extraction and Application to Human Health. In Studies in Natural Products Chemistry (1st ed., Vol. 58). Elsevier B.V. https://doi.org/10.1016/B978-0-444-64056-7.00013-1
• Arruda, H. S., Neri-Numa, I. A., Kido, L. A., Maróstica Júnior, M. R., & Pastore, G. M. (2020). Recent advances and possibilities for the use of plant phenolic compounds to manage ageing-related diseases. Journal of Functional Foods, 75, 104203. https://doi.org/10.1016/j.jff.2020.104203
• Axiotis, E., Petrakis, E. A., Halabalaki, M., & Mitakou, S. (2020). Phytochemical Profile and Biological Activity of Endemic Sideritis sipylea Boiss. in North Aegean Greek Islands. Molecules, 25(9). https://doi.org/10.3390/molecules25092022
• Bardakci, H., Cevik, D., Barak, T. H., Gozet, T., Kan, Y., & Kirmizibekmez, H. (2020). Secondary metabolites, phytochemical characterization and antioxidant activities of different extracts of Sideritis congesta P.H. Davis et Hub.-Mor. Biochemical Systematics and Ecology, 92, 104120. https://doi.org/10.1016/j.bse.2020.104120
• Berker, K., Güçlü, K., Tor, I., Demirata, B., & Apak, R. (2010). Total Antioxidant Capacity Assay Using Optimized Ferricyanide/Prussian Blue Method. Food Analytical Methods, 3, 154–168. https://doi.org/10.1007/s12161-009-9117-9
• Bilginoğlu, E. (2017). The Investigation on Drug Yield and Some Quality Characteristics of Mountain Tea (Sideritis congesta) Cultivated in Turkey. International Journal of Secondary Metabolite, 4(1), 264–269. https://doi.org/10.21448/ijsm.372482
• Celep, E., Seven, M., Akyüz, S., İnan, Y., & Yesilada, E. (2019). Influence of extraction method on enzyme inhibition, phenolic profile and antioxidant capacity of Sideritis trojana Bornm. South African Journal of Botany, 121, 360–365. https://doi.org/10.1016/j.sajb.2018.11.026
• Ceylan, Z., Meral, R., Kose, S., Sengor, G., Akinay, Y., Durmus, M., & Ucar, Y. (2020). Characterized nano-size curcumin and rosemary oil for the limitation microbial spoilage of rainbow trout fillets. LWT, 134, 109965. https://doi.org/10.1016/j.lwt.2020.109965
• Chemat, F., Abert Vian, M., & Zill-E-Huma. (2009). Microwave assisted - separations: Green chemistry in action. In Green Chemistry Research Trends.
• Ertas, A., & Yener, I. (2020). A comprehensive study on chemical and biological profiles of three herbal teas in Anatolia; rosmarinic and chlorogenic acids. South African Journal of Botany, 130, 274–281. https://doi.org/10.1016/j.sajb.2020.01.008
• González-Burgos, E., Carretero, M. E., & Gómez-Serranillos, M. P. (2011). Sideritis spp.: Uses, chemical composition and pharmacological activities - A review. Journal of Ethnopharmacology, 135(2), 209 225. https://doi.org/10.1016/j.jep.2011.03.014
• Gupta, P., & De, B. (2017). Differential responses of cell wall bound phenolic compounds in sensitive and tolerant varieties of rice in response to salinity. Plant Signaling & Behavior, 12(10). https://doi.org/10.1080/15592324.2017.1379643
• Irakli, M., Tsifodimou, K., Sarrou, E., & Chatzopoulou, P. (2018). Optimization infusions conditions for improving phenolic content and antioxidant activity in Sideritis scardica tea using response surface methodology. Journal of Applied Research on Medicinal and Aromatic Plants, 8, 67–74. https://doi.org/10.1016/j.jarmap.2017.12.001
• Kara, M., Sahin, H., Turumtay, H., Dinc, S., & Gumuscu, A. (2014). The Phenolic Composition and Antioxidant Activity of Tea with different Parts of Sideritis condensate at Different Steeping Conditions. Journal of Food and Nutrition Research, 2(5), 258–262. https://doi.org/10.12691/jfnr-2-5-8
• Magalhaes, L., Segundo, M., Reis, S., & Lima, J. (2008). Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta, 613, 1–19. https://doi.org/10.1016/j.aca.2008.02.047
• Marchev, A. S., Vasileva, L. V, Amirova, K. M., Savova, M. S., Koycheva, I. K., Balcheva-Sivenova, Z. P., Vasileva, S. M., & Georgiev, M. I. (2021). Rosmarinic acid - From bench to valuable applications in food industry. Trends in Food Science & Technology. https://doi.org/10.1016/j.tifs.2021.03.015
• Martelli, G., & Giacomini, D. (2018). Antibacterial and antioxidant activities for natural and synthetic dual-active compounds. European Journal of Medicinal Chemistry, 158, 91–105. https://doi.org/10.1016/j.ejmech.2018.09.009
• Miah, S., Fukiage, S., Begum, Z. A., Murakami, T., Mashio, A. S., Rahman, I. M. M., & Hasegawa, H. (2020). A technique for the speciation analysis of metal-chelator complexes in aqueous matrices using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry. Journal of Chromatography A, 1630, 461528. https://doi.org/10.1016/j.chroma.2020.461528
• Ognyanov, M., Remoroza, C. A., Schols, H. A., Petkova, N. T., & Georgiev, Y. N. (2021). Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.). Carbohydrate Polymers, 260, 117798. https://doi.org/10.1016/j.carbpol.2021.117798
• Perron, N. R., & Brumaghim, J. L. (2009). A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochemistry and Biophysics, 53(2), 75–100. https://doi.org/10.1007/s12013-009-9043-x
• Rodrigues, J. S., do Valle, C. P., Uchoa, A. F. J., Ramos, D. M., da Ponte, F. A. F., Rios, M. A. de S., de Queiroz Malveira, J., & Pontes Silva Ricardo, N. M. (2020). Comparative study of synthetic and natural antioxidants on the oxidative stability of biodiesel from Tilapia oil. Renewable Energy, 156, 1100 1106. https://doi.org/10.1016/j.renene.2020.04.153
• Sammani, M. S., Clavijo, S., & Cerdà, V. (2021). Recent, advanced sample pretreatments and analytical methods for flavonoids determination in different samples. TrAC Trends in Analytical Chemistry, 138, 116220. https://doi.org/10.1016/j.trac.2021.116220
• Sarikurkcu, C., Locatelli, M., Mocan, A., Zengin, G., & Kirkan, B. (2020). Phenolic profile and bioactivities of Sideritis perfoliata L.: The plant, its most active extract, and its broad biological properties. Frontiers in Pharmacology, 10, 1642. https://doi.org/10.3389/fphar.2019.01642
• Semiz, G., & Özel, M. Z. (2017). Essential oil composition of endemic Sideritis leptoclada O. Schwarz& P. H. Davis (Lamiaceae) from Turkey by using two-dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GCxGC-TOF/MS). International Journal of Secondary Metabolite, 4(2), 137–137. https://doi.org/10.21448/ijsm.309535
• Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. B. T.-M. in E. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 178 184. https://doi.org/10.1016/S0076 6879(99)99017-1
• Xu, X., Liu, A., Hu, S., Ares, I., Martínez-Larrañaga, M.-R., Wang, X., Martínez, M., Anadón, A., & Martínez, M.-A. (2021). Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action. Food Chemistry, 353, 129488. https://doi.org/10.1016/j.foodchem.2021.129488
• Zhou, F., Jongberg, S., Zhao, M., Sun, W., & Skibsted, L. H. (2019). Antioxidant efficiency and mechanisms of green tea, rosemary or maté extracts in porcine Longissimus dorsi subjected to iron-induced oxidative stress. Food Chemistry, 298, 125030. https://doi.org/10.1016/j.foodchem.2019.125030
• Zyzelewicz, D., Kulbat-Warycha, K., Oracz, J., & Zyelewicz, K. (2020). Polyphenols and Other Bioactive Compounds of Sideritis Plants and Their Potential Biological Activity. Molecules, 25(16). https://doi.org/10.3390/molecules25163763