Promising antioxidant activity of crude extract from Calliandra tweedii Benth

Year 2023, Volume: 10 Issue: 1, 59 - 70, 26.03.2023

Kátia Dos Santos Alice Nagai Carmen Palacios Bruno Evangelista Carlos Priante Débora Zamban Claudia Furlan

https://doi.org/10.21448/ijsm.953427

Cited By: 1

Abstract

The interest in medicinal plants encourages new research studies on plant extracts, as they are sources for treatments in medicine, agriculture, and veterinary. Calliandra tweedii is a native Brazilian species with broad use in folk medicine. This study aimed to examine the leaf crude extract (CE) of C. tweedii and its methanolic (MP) and hexane (HP) phases concerning total phenolic compounds (TPC), proanthocyanidins (PRO), and flavonoid profile, and correlate it to their antioxidant activity (DPPH and FRAP). CE and MP revealed high contents of TPC, PRO and high antioxidant activity (DPPH and FRAP) in relation to HP. Contrarily, HP showed reduced antioxidant activity, according to the scarce phenolic constituents obtained from the partition. Five flavonols were detected by HPLC-UV-DAD, being quercitrin the major constituent. Also, one kaempferol derivate and a series of three quercetin derivates were detected. Strong positive correlations were observed between DPPH, FRAP, and phenolic compounds. These discoveries are important to highlight the promising antioxidant activity of CE of C. tweedii, which could contribute to the expansion of popular herbal medicines and new drug discovery.

Keywords

Medicinal plants, Flavonoid, HPLC-UV-DAD, Proanthocyanidin, Antioxidant activity

Supporting Institution

Botany Department of the University of São Paulo

Project Number

Finance Code 001

Thanks

CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil - Finance Code 001)

References

• Adaramoye, O., Erguen, B., Oyebode, O., Nitzsche, B., Höpfner, M., Jung, K., Rabien, A. (2015). Antioxidant, antiangiogenic and antiproliferative activities of root methanol extract of Calliandra portoricensis in human prostate cancer cells. Journal of integrative medicine, 13, 185-193. https://doi.org/10.1016/S2095-4964(15)60175-3
• Addisu, S. (2016). Effect of dietary tannin source feeds on ruminal fermentation and production of cattle; a review. Online Journal of Animal and Feed Research, 6, 45-56.
• Aguwa, C.N., Lawal, A.M. (1988). Pharmacologic studies on the active principles of Calliandra portoricensis leaf extracts. Journal of ethnopharmacology, 22, 63-71. https://doi.org/10.1016/0378-8741(88)90231-0
• Ahn, J. H., Elliott, R., Norton, B. W. (1997). Oven drying improves the nutritional value of Calliandra calothyrsus and Gliricidia sepium as supplements for sheep given low‐quality straw. Journal of the Science of Food and Agriculture, 75, 503-510. https://doi.org/10.1002/(sici)1097-0010(199712)75:4<503::aid-jsfa905>3.0.co;2-t
• Araujo Sajamí, S.D.R. (2010). Especies forestales protectoras de riberas de cauces de agua en la provincia de Leoncio Prado.
• Cho, M., Heo Y., Park, M., Oh H. Park J., Woo, I., Ju J., Park S., Kim H., Min J. (2009). Grape seed proanthocyanidin extract (GSPE) attenuates collagen-induced arthritis. Immunology Letters, 124, 102–110. https://doi.org/10.1016/j.imlet.2009.05.001
• Chen, Z., Bertin, R., Froldi, G. (2013). EC50 estimation of antioxidant activity in DPPH assay using several statistical programs. Food Chemistry, 138, 414 420. https://doi.org/10.1016/j.foodchem.2012.11.001
• Cincin, Z. B., Unlu, M., Kiran, B., Bireller, E.S., Baran, Y., Cakmakoglu, B. (2014). Molecular mechanisms of quercitrin-induced apoptosis in non-small cell lung cancer. Archives of medical research, 45, 445-454. https://doi.org/10.1016/j.arcmed.2014.08.002
• Da Silva, B.P., Parente, J.P. (2013). A new complex triterpenoid saponin from Calliandra pulcherrima with haemolytic activity and adjuvant effect. Phytochemistry Letters, 6, 633-639. https://doi.org/10.1016/j.phytol.2013.08.009
• Dewick, P.M. 2009. Medicinal natural products: a biosynthetic approach. John Wiley & Sons, UK, 3rd edition.
• Encarnación, R.D., Keer S.G. (1991). Antimicrobial screening of medicinal plants from Baja California Sur, Mexico. Journal of Ethnopharmacology, 31, 181 192. https://doi.org/10.1016/0378-8741(91)90004-W
• Encarnación, R.D., Ochoa, N.A. (1994). Two new flavones from Calliandra Californica. Journal of Natural Products, 57, 1307-1309. https://doi.org/10.1021/np50111a023
• Farnsworth, N.R., Akerele, O., Bingel, A.S., Soejarto, D.D., Guo, Z. (1985). Medicinal plants in therapy. Bulletin of the world health organization, 63, 965.
• Ferreira de Souza, D.B, Araújo, G.M.L., Monteiro, W. (1999). First national report for the convention on biological diversity - Brazil. Ministério do Meio Ambiente-MMA.
• Firmansyah, M.A., Jayanegara, A., Wijayanto, N. (2019). In vitro biological control of Ceratobasidium ramicola by using tannin extracts from Acacia villosa, Myristica fragrans, Acacia mangium, and Calliandra calothyrsus leaves. Brazilian Journal of Biology, (AHEAD). https://doi.org/10.1590/1519-6984.184912
• Flora do Brasil 2020 em construção. Jardim Botânico do Rio de Janeiro. Disponível em: http://floradobrasil.jbrj.gov.br/. Accessed on 16 June 2022 Furlan, C.M., Santos, K.P., Sedano-Partida, M.D., da Motta, L.B., Santos, D.Y.A., Salatino, M.L.F., Negri, G., Berry, P.E., van Ee, B.W., Salatino, A. (2015). Flavonoids and antioxidant potential of nine Argentinian species of Croton (Euphorbiaceae). Brazilian Journal of Botany, 38, 693-702. https://doi.org/10.1007/s40415-014-0115-9
• Gómez-Florit, M., Monjo, M., Ramis, J.M. (2014). Identification of quercitrin as a potential therapeutic agent for periodontal applications. Journal of Periodontology, 85, 966-974. https://doi.org/10.1902/jop.2014.130438
• Hess, H.D., Valencia, F.L., Monsalve, L.M., Lascano, C.E., Kreuzer, M. (2004). Effects of tannins in Calliandra calothyrsus and supplemental molasses on ruminal fermentation in vitro. Journal of Animal and Feed Sciences, 13, 95 98. https://doi.org/10.22358/jafs/73747/2004
• Huang, D., Ou, B., Prior, R.L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53, 1841 1856. https://doi.org/10.1021/jf030723c
• Krishnaiah, D., Sarbatly, R., Nithyanandam, R. (2011). A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing, 89, 217 233. https://doi.org/10.1016/j.fbp.2010.04.008
• Kumar, S., Agrawal, V., Gupta, S.C. (2002). Somatic embryogenesis in the woody legume Calliandra tweedii. Plant Cell, Tissue and Organ Culture, 71, 77 80. https://doi.org/10.1023/A:1016518412637
• Lorenzi, H., Souza, H.M. (2001). Plantas ornamentais no Brasil – Arbustivas, herbáceas e trepadeiras. 3 ed. Plantarum, Nova Odessa, 1088p.
• Mabry, T.J., Markham, K.R., Thomas, M.B. (1970). The systematic identification of flavonoids. Springer.
• Markham, K.R. (1982). Techniques of flavonoid identification (Vol. 36). London: Academic press.
• Moharram, F.A., Marzouk, M.S., Ibrahim, M.T., Mabry, T.J. (2006). Antioxidant galloylated flavonol glycosides from Calliandra Haematocephala, 20, 927 934. https://doi.org/10.1080/14786410500378494
• Orishadipe, A.T., Okogun, J.I., Mishelia, E. (2010). Gas chromatography-mass spectrometry analysis of the hexane extract of Calliandra portoricensis and its antimicrobial activity. African Journal of Pure and Applied Chemistry, 4, 131-134.
• Paiva, P.D.O. (2003). Características das principais plantas ornamentais utilizadas em paisagismo. Editora UFLA, Lavras.
• Rakhmani, S., Brooker, J.D., Jones, G.P., Palmer, B. (2005). Composition of condensed tannins from Calliandra calothyrsus and correlation with in sacco digestibility. Animal Feed Science and Technology, 121, 109-124. https://doi.org/10.1016/j.anifeedsci.2005.02.010
• Riswan, S., Gintings, A.N., Samsoedin, I. (1996). Historical introduction of Calliandra in Indonesia. In Proceedings of the International Workshop on the Genus Calliandra. Forest, Farm and Community Tree Research Reports (Special Issue). Winrock International, Morrilton, Arkansas, USA (pp. 18-29).
• Silva, E.R., Maquiaveli, C.C., Magalhães, P.P. (2012). The leishmanicidal flavonols quercetin and quercitrin target Leishmania (Leishmania) amazonensis arginase. Experimental Parasitology, 130, 183-188. https://doi.org/10.1016/j.exppara.2012.01.015
• Sikder, M.A.A., Millat, M.S., Sultana, A., Kaisar, M.A., Rashid, M.A. (2012). In vitro membrane stabilizing activity, total phenolic content, cytotoxic, thrombolytic and antimicrobial activities of Calliandra surinamensis (Wall.). Journal of Pharmacognosy and Phytochemistry, 1, 45-50.
• Souza, E. R. (2015). Calliandra in Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB22845
• Taiz, L., Zeiger E. (2009). Plant Physiology, Sunderland: Sinauer Associates. 690 pp.
• Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 39, 44 84. https://doi.org/10.1016/j.biocel.2006.07.001
• Waterman, P.G., Mole, S. (1994). Analysis of Phenolic Plant Metabolites. Blackwell Scientific Publications, Oxford.
• WHO (2019). WHO global report on traditional and complementary medicine 2019. Geneva: World Health Organization, 2019. Available at: https://www.who.int/traditional-complementary-integrative-medicine/WhoGlobalReportOnTraditionalAndComplementaryMedicine2019.pdf?ua=1
• Wei, S., Chen, H., Lin, Y. (2015). Comparison of chemical compositions and antioxidant activities of condensed tannins from different parts of Calliandra haematocephala. Journal of Wood Chemistry and Technology, 35, 193 206. https://doi.org/10.1080/02773813.2014.919596
• Yousef, M.I., Saad, A.A., El-Shennawy, L.K. (2009). Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food and Chemical Toxicology, 47, 1176–1183. https://doi.org/10.1016/j.fct.2009.02.007
• Yamazaki, E., Inagaki, M., Kurita, O., Inoue, T. (2007). Antioxidant activity of Japanese pepper (Zanthoxylum piperitum DC.) fruit. Food Chemistry, 100, 171 177. https://doi.org/10.1016/j.foodchem.2005.09.036