Unrevealing of the Effect of Olfactory Bulb Lesion on Histomorphological Changes in Sublingual Glands: An Experimental Study

Year 2019, Volume: 3 Issue: 2, 23 - 28, 15.12.2019

Mehmetnuri Koçak Mehmet Dumlu Aydın Zülküf Kaya Erdem Karadeniz Atıf Bayramoğlu Ali Ahıskalıoğlu Nazan Aydın Tuba Demirci

Abstract

This study was performed to examine basic neuropathological mechanisms of undetermined
effects of olfactory bulb lesion in sublingual glands. Twenty-six adult rats were randomized
in three groups as control group (Group I, n=8), Sham group (Group II, n=7) and olfactory
bulb lesion (OBL) group (Group III, n=11), and they all observed for the duration of eight
weeks and finally euthanized. The olfactory bulbs of rat brains and sublingual glands (SLG),
were extracted bilaterally. Samplings stained with hematoxylin/eosin and TUNEL stains to
assess the stereogical olfactory bulbs volume loss degrees, and follicular volume values
(FVV) of SLG per cubic centimeters. The estimated mean volumes of olfactory bulbs were
as 4.87±0.33/mm3 in group I, 4.32±0.43/mm3 in group II, and 2.12±0.19/mm3 in group III
(p<0.0001 and p<0.0005; in Group III vs. I, Group III vs. II, respectively). The mean volumes
of FVV and degenerated neuron density of SLG were 215±56×106µm3/cm3 and 13±3/mm3
in Group I, 193 ± 44×106µm3/cm3 and 62±11/mm3 in Group II (p<0.005), 134±27×106
µm3/cm3 and 346±83/mm3 in Group III (p<0.0001 and p<0.0005; in Group III vs. I, Group
III vs. II, respectively). This study suggests that OBL may be responsible for sublingual gland
degeneration, which has not been stated so far.

Keywords

Olfaction, Olfactory Bulb Lesion, Sublingual Gland, Facial Nerve, Experimental study

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