eur j biol European Journal of Biology 2602-2575 2618-6144 Istanbul University Determination of Optimum Immobilization Conditions of Trametes versicolor Laccase with Sodium Alginate Beads Aljinat Boncukları ile Trametes versicolor Lakkazının Optimum İmmobilizasyon Koşullarının Belirlenmesi Ünal Arzu Kolankaya Nazif 05 12 2014 72 2 15 21 05 12 2014 Copyright © 1940, European Journal of Biology 1940 European Journal of Biology

Laccase (EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmentalpollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrialapplications can be increased by immobilizing the enzyme on a carrier. In the present study, laccaseobtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappacarrageenanand the effect of contact time, pH, temperature, the amount of carrier and enzymeconcentration were investigated to determine optimum conditions of laccase immobilization. Sodiumalginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximumlaccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200mg of sodium alginate amount.

Lakkaz (EC 1.10.3.2), fenolik bileşikler gibi çeşitli çevresel kirleticilerin oksidasyonunu katalizleyen çoklu bakır enzimidir. Çevresel ve endüstriyel uygulamalar için bir taşıyıcı üzerinde enzimin immobilize edilmesiyle etkinliği arttırılabilir. Bu çalışmada, Trametes versicolor’dan elde edilen lakkaz sodium aljinat ve kappa-karagen üzerinde immobilize edilmiş, temas süresi, pH, sıcaklık, taşıyıcı miktarı, enzim konsantrasyonun etkisi değerlendirilmiştir. Sodyum aljinat ve kappa-karragenanın immobilizasyon etkinliği araştırılmıştır. Sonuç olarak, sodyum aljinat boncukları yüksek immobilizasyon veriminden dolayı en iyi taşıyıcı olarak seçilmiştir. Maksimum lakkaz immobilizasyonu, 30 dakika temas süresi, pH 4.5, 30 °C, 200 mg sodyum aljinat koşullarında elde edilmiştir.

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