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Yeast Diversity in the Mangrove Sediments of North Kerala, India

Year 2022, , 50 - 57, 30.06.2022
https://doi.org/10.26650/EurJBiol.2022.1027475

Abstract

Objective: Mangrove sediments, due to their unique environment, are considered to be a crucial habitat in identifying yeast strains with potent industrial, biotechnological and bioremediation properties. The goal of the current study was to understand the presence, diversity and hydrolytic enzyme properties of yeasts from the mangrove sediments of North Kerala. Materials and Methods: Sampling was done during the period 2018-2020 from mangrove sediments of 5 districts along the North Kerala coast. Isolation of yeast was done on yeast glucose peptone agar, and isolates were tested for their potential for the production of extracellular enzymes viz. amylase, cellulase, chitinase, DNase, lipase, ligninase, pectinase, protease and urease, using standard media with specific substrates. Morphological assessment, biochemical characterization and molecular identification of the isolates were performed. The phylogenetic tree of the selected yeast strains was constructed with the Maximum Likelihood method using MEGA X software. Results: A total of 482 yeast strains belonging to 12 genera were obtained from the mangrove sediment samples, the most dominant genera being Candida (56.3%). Kluveromyces, Debaromyces, Torulaspora, Trichosporon, pigmented yeast Rhodotorula, Cyberlindnera, Wickerhamiella, Pichia, Trichoderma, Meyerozyma and Kodamaea were the other genera identified. The majority of the yeast present in the mangrove sediment samples were lipolytic (68%), followed by ureolytic (23%), ligninolytic (16%), cellulolytic (9%), DNAlytic (9%), proteolytic (8%), amylolytic (6%), pectinolytic (4%) and chitinolytic (2%) forms. All 12 genera of yeast obtained had positive forms for extracellular lipase. Conclusion: The yeast strains obtained from mangrove sediments in the study were found to be ecologically important and have great biotechnological potential.

Supporting Institution

This study was supported by Back to Lab Projects, Women Scientist Division (BLP-WSD), Kerala State Council for Science, Technology and Environment (KSCSTE), Thiruvananthapuram, India

References

  • 1. Thatoi H, Behera BC, Mishra RR, Dutta SK. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: A review. Ann Microbiol 2012; 1-22.
  • 2. Boguslawska-Was E, Dabrowski W. The seasonal variability of yeasts and yeast-like organisms in water and bottom sediment of the Szczecin Lagoon. Int J Hyg Envir Heal 2001; 203: 451-8.
  • 3. Kandasamy K, Alikunhi NM, Subramanian M. Yeasts in marine and estuarine environments. J Yeast Fungal Res 2012; 3: 74-82.
  • 4. Kutty SN, Philip R. Marine yeasts - Areview. Yeast 2008; 25(7): 465- 83.
  • 5. Pothayi V, Devasia SC. A study on the distribution and hydrolytic enzyme potential of yeasts in the mangrove sediments of Northern Kerala. Ind J Microbio Res 2020; 7: 161-7.
  • 6. Cheng YT, Yang CF. Using strain Rhodotorula mucilaginosa to produce carotenoids using food wastes. J Taiwan Inst Chem Eng 2016; 61: 270-5.
  • 7. Elsanhoty RM, Al-Turki AI, El-Razik MMA. Production of carotenoids from Rhodotorula mucilaginosa and their applications as colorant agent in sweet candy. J Food Agr Env 2017; 15: 21-6.
  • 8. Wickerham LJ. Taxonomy of yeasts. U.S. Dept Agricultural Technical Bullettin 1951; 1029: 1-19.
  • 9. Barnett JA, Payne RW, Yarrow D. Yeasts: characteristics and identification, Second ed. Cambridge University Press, Cambridge. 1990.
  • 10. Harju S, Fedosyuk H, Peterson KR. Rapid isolation of yeast genomic DNA. Bust n’Grab. BMC Biotech 2004; 4: 8-12.
  • 11. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols, A guide to methods and applications. Academic Press: San Diego, CA; 1990.
  • 12. Pagnocca FG, Mendonça-Hagler LC, Hagler AN. Yeasts associated with the white shrimp Penaeus schmitti, sediment, and water of Sepetiba Bay, Rio de Janeiro, Brazil. Yeast 1989; 5: 479-83.
  • 13. Chi ZM, Liu TT, Chi Z, Liu GL, Wang ZP. Occurrence and diversity of yeasts in the mangrove ecosystems in Fujian, Guangdong and Hainan provinces of China. Indian J Microbiol 2012; 52: 346-53.
  • 14. Fell JW, Van Uden N. Yeasts in marine environments. In: Oppenheimer C H, editors. Symposium on Marine Microbiology; Charles C Thomas Publisher, Springfield; 1963. pp. 329-40.
  • 15. Loureiro STA, de Queiroz-Cavalcanti MA, Neves RP, de Oliveira Passavante JZ et al. Yeasts isolated from sand and sea water in beaches of Olinda, Pernambuco state, Brazil. Braz J Microbiol 2005; 36: 1-8.
  • 16. Hagler AN. Yeasts as Indicators of Environmental Quality. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. p. 515-32.
  • 17. Silva-Bedoya LM, Ramírez-Castrillón M, Osorio-Cadavid E. Yeast diversity associated to sediments and water from two Colombian artificial lakes. Braz J Microbiol 2014; 45: 135-42.
  • 18. Kutty SN, Damodaran R, Philip R. Yeast isolates from the slope sediments of Arabian Sea and Bay of Bengal: Physiological characterization. Adv App Sci Res 2014; 5: 177-87.
  • 19. Fialova A, Boschke E, Bley T. Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements. Int Biodeterior Biodegrad 2004; 54: 69-76.
  • 20. Farag S, Soliman NA. Biodegradation of crude petroleum oil and environmental pollutants by Candida tropicalis strain. Braz Arc Biol Technol 2011; 54: 821-30.
  • 21. Kurtzman CP, Fell JW, Boekhout T, editors. The yeasts: A taxonomic study. USA: Elsevier; 2011. pp 2100.
  • 22. Rishipal R, Philip R. Selection of marine yeasts for the generation of single-cell protein from prawn-shell waste. Biores Technol 1998; 65: 255-6.
  • 23. Manivannan S, Alikunhi NM, Kandasamy K. In vitro Synthesis of Silver Nanoparticles by Marine Yeasts from Coastal Mangrove Sediment. Adv Sci Lett 2010; 3: 1-6.
  • 24. Raspor P, Zupan J. Yeast in extreme environments. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. pp 371-417.
  • 25. Botha A. Yeasts in soil. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. pp 221-40.
  • 26. Li M L, Chi G L, Chi Z, Chi Z M. Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass Bioenergy 2010; 34: 101-7.
  • 27. Paskevicius A. Lipase activity of yeasts and yeast-like fungi functioning under natural conditions. Biologija 2001; 4: 16-8.
  • 28. Vakhlu J, Kour A. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 2006; 9: 69-85.
  • 29. Hasan F, Shah AA, Hameed A. Industrial Applications of Microbial Lipases. Enzyme Microb Technol 2006; 39: 235-51.
  • 30. Bakhtiari MR, Faezi MG, Fallahpour M, Noohi A, Moazami N, Amidi Z. Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011. Proc Biochem 2006; 41: 547-51.
  • 31. Bharathi N, Meyyappan RM. Production of urease enzyme from ureolytic yeast cell. Int J Eng Res Genl Sci 2015; 3: 643-7.
Year 2022, , 50 - 57, 30.06.2022
https://doi.org/10.26650/EurJBiol.2022.1027475

Abstract

References

  • 1. Thatoi H, Behera BC, Mishra RR, Dutta SK. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: A review. Ann Microbiol 2012; 1-22.
  • 2. Boguslawska-Was E, Dabrowski W. The seasonal variability of yeasts and yeast-like organisms in water and bottom sediment of the Szczecin Lagoon. Int J Hyg Envir Heal 2001; 203: 451-8.
  • 3. Kandasamy K, Alikunhi NM, Subramanian M. Yeasts in marine and estuarine environments. J Yeast Fungal Res 2012; 3: 74-82.
  • 4. Kutty SN, Philip R. Marine yeasts - Areview. Yeast 2008; 25(7): 465- 83.
  • 5. Pothayi V, Devasia SC. A study on the distribution and hydrolytic enzyme potential of yeasts in the mangrove sediments of Northern Kerala. Ind J Microbio Res 2020; 7: 161-7.
  • 6. Cheng YT, Yang CF. Using strain Rhodotorula mucilaginosa to produce carotenoids using food wastes. J Taiwan Inst Chem Eng 2016; 61: 270-5.
  • 7. Elsanhoty RM, Al-Turki AI, El-Razik MMA. Production of carotenoids from Rhodotorula mucilaginosa and their applications as colorant agent in sweet candy. J Food Agr Env 2017; 15: 21-6.
  • 8. Wickerham LJ. Taxonomy of yeasts. U.S. Dept Agricultural Technical Bullettin 1951; 1029: 1-19.
  • 9. Barnett JA, Payne RW, Yarrow D. Yeasts: characteristics and identification, Second ed. Cambridge University Press, Cambridge. 1990.
  • 10. Harju S, Fedosyuk H, Peterson KR. Rapid isolation of yeast genomic DNA. Bust n’Grab. BMC Biotech 2004; 4: 8-12.
  • 11. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols, A guide to methods and applications. Academic Press: San Diego, CA; 1990.
  • 12. Pagnocca FG, Mendonça-Hagler LC, Hagler AN. Yeasts associated with the white shrimp Penaeus schmitti, sediment, and water of Sepetiba Bay, Rio de Janeiro, Brazil. Yeast 1989; 5: 479-83.
  • 13. Chi ZM, Liu TT, Chi Z, Liu GL, Wang ZP. Occurrence and diversity of yeasts in the mangrove ecosystems in Fujian, Guangdong and Hainan provinces of China. Indian J Microbiol 2012; 52: 346-53.
  • 14. Fell JW, Van Uden N. Yeasts in marine environments. In: Oppenheimer C H, editors. Symposium on Marine Microbiology; Charles C Thomas Publisher, Springfield; 1963. pp. 329-40.
  • 15. Loureiro STA, de Queiroz-Cavalcanti MA, Neves RP, de Oliveira Passavante JZ et al. Yeasts isolated from sand and sea water in beaches of Olinda, Pernambuco state, Brazil. Braz J Microbiol 2005; 36: 1-8.
  • 16. Hagler AN. Yeasts as Indicators of Environmental Quality. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. p. 515-32.
  • 17. Silva-Bedoya LM, Ramírez-Castrillón M, Osorio-Cadavid E. Yeast diversity associated to sediments and water from two Colombian artificial lakes. Braz J Microbiol 2014; 45: 135-42.
  • 18. Kutty SN, Damodaran R, Philip R. Yeast isolates from the slope sediments of Arabian Sea and Bay of Bengal: Physiological characterization. Adv App Sci Res 2014; 5: 177-87.
  • 19. Fialova A, Boschke E, Bley T. Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements. Int Biodeterior Biodegrad 2004; 54: 69-76.
  • 20. Farag S, Soliman NA. Biodegradation of crude petroleum oil and environmental pollutants by Candida tropicalis strain. Braz Arc Biol Technol 2011; 54: 821-30.
  • 21. Kurtzman CP, Fell JW, Boekhout T, editors. The yeasts: A taxonomic study. USA: Elsevier; 2011. pp 2100.
  • 22. Rishipal R, Philip R. Selection of marine yeasts for the generation of single-cell protein from prawn-shell waste. Biores Technol 1998; 65: 255-6.
  • 23. Manivannan S, Alikunhi NM, Kandasamy K. In vitro Synthesis of Silver Nanoparticles by Marine Yeasts from Coastal Mangrove Sediment. Adv Sci Lett 2010; 3: 1-6.
  • 24. Raspor P, Zupan J. Yeast in extreme environments. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. pp 371-417.
  • 25. Botha A. Yeasts in soil. Gábor P, Rosa C, editors. Biodiversity and Ecophysiology of Yeasts. Berlin: Springer; 2006. pp 221-40.
  • 26. Li M L, Chi G L, Chi Z, Chi Z M. Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass Bioenergy 2010; 34: 101-7.
  • 27. Paskevicius A. Lipase activity of yeasts and yeast-like fungi functioning under natural conditions. Biologija 2001; 4: 16-8.
  • 28. Vakhlu J, Kour A. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 2006; 9: 69-85.
  • 29. Hasan F, Shah AA, Hameed A. Industrial Applications of Microbial Lipases. Enzyme Microb Technol 2006; 39: 235-51.
  • 30. Bakhtiari MR, Faezi MG, Fallahpour M, Noohi A, Moazami N, Amidi Z. Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011. Proc Biochem 2006; 41: 547-51.
  • 31. Bharathi N, Meyyappan RM. Production of urease enzyme from ureolytic yeast cell. Int J Eng Res Genl Sci 2015; 3: 643-7.
There are 31 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Pothayi Vidya This is me 0000-0003-0863-1280

Chempakassery Devasia Sebastian 0000-0001-9555-0627

Publication Date June 30, 2022
Submission Date November 24, 2021
Published in Issue Year 2022

Cite

AMA Vidya P, Sebastian CD. Yeast Diversity in the Mangrove Sediments of North Kerala, India. Eur J Biol. June 2022;81(1):50-57. doi:10.26650/EurJBiol.2022.1027475