The role of prostaglandin E(1) on the model of acute ischemic reperfusion-induced renal injury in rats

Year 2016, Volume: 75 Issue: 2, 7 - 17, 22.08.2017

Nurcan Orhan , Şehnaz Bolkent

Abstract

Prostaglandin E(1) is a natural prostaglandin that has various pharmacological effects. It has been shown
that prostaglandin E(1) has a protective action on some organs of rats treated with renal ischemia/
reperfusion. Our aim was to investigate the role of prostaglandin E(1) in rats with renal ischemia-
reperfusion-induced acute renal injury. Histological, immunohistochemical, and biochemical analyses
were performed. Sprague Dawley male rats were divided into four groups in this study. The first group
was given physiological saline only. Second group was administered prostaglandin E(1) (20 μg/kg) only.
Third group was treated with ischemia-reperfusion. Fourth group was administered prostaglandin E(1)
(20 μg/kg) and applied ischemia-reperfusion. All the rats were sacrificed after the reperfusion period.
Dissected kidney tissue was used for histological examination and biochemical analysis. The kidneys
of the experimental group with ischemia-reperfusion model have shown histopathologic changes as
an increase in proliferating cell nuclear antigen (PCNA) and caspase 3 immunoreactivity, a significant
decrease in reduced glutathione and DNA levels, and a significant increase in lipid peroxidation levels.
In contrast, the administration of prostaglandin E(1) reversed these effects on kidneys of the animals
applied ischemia-reperfusion model. As a summary, the histological, immunohistochemical, and
biochemical evaluations have revealed that prostaglandin E(1) ameliorated renal damage of rats with
renal ischemia/reperfusion. 

Keywords

Acute ischemia-reperfusion, apoptosis, proliferation, prostaglandin E(1), rat

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