Year 2020, Volume 79 , Issue 2, Pages 144 - 150 2020-12-25

The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes

Sezen BILEN OZYUREK [1] , Nermin Hande AVCİOGLU [2]


Objective: Fungi play an important role in the removal of hazardous organic compounds from the environment with their extracellular multiple enzyme systems. In the bioremediation processes, fungi act as a bioreactor by breaking down or are biosorbent by accumulating organic pollutants.
Materials and Methods: The aim of this study was to investigate and to compare the biodegradation and biosorption capacities of different amounts of live and dead biomasses and different concentrations of culture supernatants of Aspergillus parasiticus NRRL:3386 with gravimetric, gas chromatography-mass spectrometry (GC/MS) and scanning electron microscope analyses (SEM).
Results: This study indicated that 1 g of live biomass degraded 80% of petroleum within 4-days of incubation. The cell-free culture supernatant was not as effective as the live biomass in petroleum degradation. The petroleum biosorption was achieved at over 50% by 1 g and 2.5 g; over 70% by 5 g and 7.5 g, and over 80% by 10 g of dead biomasses. The petroleum removal efficiencies of 2 g of live and 10 g of dead biomasses were over 80%. GC/MS analysis demonstrated that C10 - C18 n-alkanes (except C11 and C13) and C11, C13, C19 - C26 n-alkanes were degraded 47 - 77% and over 80%, respectively. The most striking result was that C27 - C33 n-alkanes were efficiently degraded over 90% in a short incubation period. It was clearly shown by SEM analysis that the gaps between fungal hyphae were clear and bright before biosorption of petroleum, whereas the gaps between fungal hyphae were closed after biosorption of petroleum.
Conclusion: The results clearly pointed out that A. parasiticus NRRL:3386 will make a significant contribution to advanced mycoremediation studies.
Aspergillus parasiticus NRRL:3386, petroleum, biosorption, biodegradation, Gas Chromatography- Mass Spectrometry (GC/MS), Scanning Electron Microscope (SEM)
  • Referans1 Zhang JH, Xue QH, Gao H, Ma X, Wang P. Degradation of crude oil by fungal enzyme preparation from Aspergillus spp. For potential use in enhanced oil recovery. J Chem Technol Biotechnol 2016, 91: 865-875.
  • Referans2 Fan CY, Krishnamurth S. Enzymes for enhancing bioremediation of petroleum-contaminated soils: A brief review. J Air Waste Manag Assoc 1995, 45: 453-459.
  • Referans3 Balaji V, Aruzlazhagan P, Ebenezer P. Enzymatic bioremediation of polyaromatic hydrocarbons by fungal consortia enriched from petroleum contaminated soil and seeds. J Environ Biol 2014, 35: 1-9.
  • Referans4 Al-Hawash AB, Zhang X, Ma F. Removal and biodegradation of different petroleum hydrocarbons using filamentous fungus Aspergillus sp. RFC-1. Microbiologyopen 2019, 8(1): 1-14.
  • Referans5 Islam NF. Bioprospecting fungal diversity from crude oil infiltrate soil of Assam, India’s Northeast. Trop Plant Res 2017, 4(2): 319-329.
  • Referans6 Al‐Nasrawi H. Biodegradation of crude oil by fungi isolated from Gulf of Mexico. J Bioremediat Biodegrad 2012, 3(4): 1–6.
  • Referans7.AI-Jawhari IFH. Ability of some soil fungi in biodegradation of petroleum hydrocarbon. J Appl Environ Microbiol 2014, 2(2): 46–52.
  • Referans8 Vanishree M, Thatheyu A.J, Ramya D. Biodegradation of petroleum using the fungus Penicillium sp. Forensic Sci Int 2014, 2(1): 26-31.
  • Referans9 Dawoodi V, Madani M, Tahmourespour A, Golshani Z. The study of heterotrophic and crude oil-utilizing soil fungi in crude oil contaminated regions. J Bioremediat Biodegrad 2015, 6(2): 1-5.
  • Referans10 Al-Hawash AB, Alkooranee JT, Abbood HA, Zhang J, Sun, J, Zhang X, Ma F. Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq Biotechnol Rep 2018, 17: 104-109.
  • Referans11 Bilen Ozyurek S, Seyis Bilkay I. Biodegradation of petroleum by Klebsiella pneumoniae isolated from drilling fluid. Int J Environ Sci Technol 2018, 15: 2107-2116.
  • Referans12 Bilen Ozyurek S, Seyis Bilkay I. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit. SN Applied Sciences 2020, 272.
  • Referans13 Maddela NR, Scalvenzi L, Perez M, Montero C, Gooty JM. Efficiency of indigenous filamentous fungi for biodegradation of petroleum hydrocarbons in medium and soil: Laboratory study from Ecuador. Bull Environ Contam Toxicol 2015, 95: 385-394.
  • Referans14 Thenmozhi R, Arumugam K, Nagasathya A, Thajuddin N, Paneerselvam A. Studies on mycoremediation of used engine oil contaminated soil samples. Adv Appl Sci Res 2013, 4(2): 110-118.
  • Referans15 Ding J, Chen BL, Zhu LZ. Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution. Chin Sci Bull 2013, 58: 613-621.
  • Referans16 Chergui A, Kerbachi R, Junter G-A. Biosorption of hexacyanoferrate (III) complex anion to dead biomass of the basidiomycete Pleurotus mutilus: Biosorbent characterization and batch. Chem Eng J 2009, 147: 150-160.
  • Referans17 Zhang J, Zhang X, Liu J, Li R, Shen B. Isolation of a thermophilic bacterium Geobacillus sp. SH-1, capable of degrading aliphatic hydrocarbons and naphthalene simultaneously, and identification of its naphthalene degrading pathway. Bioresour Technol 2012, 124: 83-89.
  • Referans18 Hadibarata T, Tachibana S. Microbial degradation of n-eicosane by filamentous fungi. In: Interdisciplinary studies on environmental chemistry and environmental research in Asia. (Eds. Y. Obayashi, T. Isobe, A. Subramanian, S. Suzuki and S. Tanabe), Terrapub, Tokyo, 2009, 323-329.
  • Referans19 Covino S, D'Annibale A, Stazi R, Cajthaml T, Čvančarová M, Stella T, Petruccioli M. 2Assessment of degradation potential of aliphatic hydrocarbons by autochthonous filamentous fungi from a historically polluted clay soil. Sci Total Environ 2015, 505: 545-554.
Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Orcid: 0000-0002-5056-7051
Author: Sezen BILEN OZYUREK
Institution: HACETTEPE UNIVERSITY
Country: Turkey


Orcid: 0000-0003-2243-5817
Author: Nermin Hande AVCİOGLU (Primary Author)
Institution: HACETTEPE ÜNİVERSİTESİ, FEN FAKÜLTESİ
Country: Turkey


Dates

Application Date : September 30, 2020
Acceptance Date : November 6, 2020
Publication Date : December 25, 2020

Bibtex @research article { iufsjb802150, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2020}, volume = {79}, pages = {144 - 150}, doi = {}, title = {The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes}, key = {cite}, author = {Bılen Ozyurek, Sezen and Avcioglu, Nermin Hande} }
APA Bılen Ozyurek, S , Avcioglu, N . (2020). The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes . European Journal of Biology , 79 (2) , 144-150 . Retrieved from https://dergipark.org.tr/en/pub/iufsjb/issue/55111/802150
MLA Bılen Ozyurek, S , Avcioglu, N . "The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes" . European Journal of Biology 79 (2020 ): 144-150 <https://dergipark.org.tr/en/pub/iufsjb/issue/55111/802150>
Chicago Bılen Ozyurek, S , Avcioglu, N . "The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes". European Journal of Biology 79 (2020 ): 144-150
RIS TY - JOUR T1 - The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes AU - Sezen Bılen Ozyurek , Nermin Hande Avcioglu Y1 - 2020 PY - 2020 N1 - DO - T2 - European Journal of Biology JF - Journal JO - JOR SP - 144 EP - 150 VL - 79 IS - 2 SN - 2602-2575-2618-6144 M3 - UR - Y2 - 2020 ER -
EndNote %0 European Journal of Biology The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes %A Sezen Bılen Ozyurek , Nermin Hande Avcioglu %T The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes %D 2020 %J European Journal of Biology %P 2602-2575-2618-6144 %V 79 %N 2 %R %U
ISNAD Bılen Ozyurek, Sezen , Avcioglu, Nermin Hande . "The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes". European Journal of Biology 79 / 2 (December 2020): 144-150 .
AMA Bılen Ozyurek S , Avcioglu N . The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes. Eur J Biol. 2020; 79(2): 144-150.
Vancouver Bılen Ozyurek S , Avcioglu N . The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes. European Journal of Biology. 2020; 79(2): 144-150.
IEEE S. Bılen Ozyurek and N. Avcioglu , "The Role of Aspergillus parasiticus NRRL: 3386 Strain on Petroleum Biodegradation and Biosorption Processes", European Journal of Biology, vol. 79, no. 2, pp. 144-150, Dec. 2020