GENERATION OF SILK FIBROIN-CA-P COMPOSITE BIOMIMETIC BONE REPLACEMENT MATERIAL USING ELECTROCHEMICAL DEPOSITION

Year 2017, Volume: 17 Issue: 2, 3439 - 3443, 27.07.2017

Sermin Utku , Kubra Basaran Yagmur Sunar Hulya Celebıoglu İbrahim Kapıcı

Abstract

Mineralized natural protein based novel bone replacement materials are
investigated for tissue engineering. Mineralized silk fibroin composite foams
and films display excellent biocompatibility. In this study, the biomimetic and
electrochemical mineralization of orderly oriented silk fibroin scaffolds was
studied.

Commercially obtained pure silk woven fabric was boiled in 0.02 M Na₂CO₃
for 20 min. Calcium phosphate was deposited at 37°C for twenty minutes in seven
sequential immersion steps, using 250 mM CaCl₂ 2H₂O and
120 mM K₂HPO₄, containing 0.15 M NaCl and 50mM TRIS-HCl,
pH 7.4, followed by electrochemical treatment in modified SBF solution at 40°C
at a current density of -25mA/cm² for 60 min.

The amount of biomimetically deposited Ca-P increased with the number
of immersion steps. SEM images and XRD analysis of the Ca-P deposit indicated the
initial formation of brushite with its monoclinic crystal structure and characteristic
peak at 11.76 2θ, and electrochemical conversion of brushite to hydroxyapatite
on silk after electrochemical cathodization as confirmed by XRD and SEM
analysis.

Thus, a silk-fibroin-hydroxyapatite composite material prepared as a xenograft
consisting of biocompatible components, and easily prepared as an economical bone
segment replacement material with highly oriented fibers.

Keywords

silk-fibroin-hydroxyapatite scaffold, composite biomaterials, bone tissue engineering, bone replacement materials

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