Research Article
BibTex RIS Cite

Evaluation of Strobilurin and Triazole-Based Fungicides for Managing Foliar Blight in Large Cardamom in Taplejung, Nepal

Year 2025, Volume: 8 Issue: 2, 76 - 86

Abstract

Large cardamom (Amomum subulatum Roxb.) is an important spice crop in the Eastern Himalayas, including Nepal, but its productivity is severely affected by Colletotrichum Leaf Blight (CLB) caused by Colletotrichum spp. To manage this disease, a field experiment was conducted using a Randomized Complete Block Design (RCBD) with seven treatments and three replications. The treatments included three fungicides: Nativo (Tebuconazole 50% + Trifloxystrobin 25% WG), Syodia (Azoxystrobin 11% + Tebuconazole 18% SC), and Symister Top (Azoxystrobin 18.2% + Difenoconazole 11.4% SC): each applied at two different concentrations (1 g/ml per liter and a lower dose), along with a control. Before treatment, 40 leaves were randomly selected to assess initial disease severity. Fungicides were applied using a high-volume knapsack sprayer at 4 liters per plot. A second spray was done 15 days after the first when disease severity reached about 5%. All treatments followed recommended agronomic practices. Among the treatments, Symister Top at 1 ml/L was the most effective, reducing disease severity by 68.4%, followed by Nativo (61.7%) and Syodia (57.9%). The highest yield increase (22.5%) was also recorded with Symister Top. The efficacy is due to the systemic and translaminar action of the fungicides, though the risk of resistance development remains a concern. The study suggests that appropriate fungicide use can effectively manage CLB in large cardamom. However, integrating chemical control with other sustainable practices is essential for long-term disease management and environmental safety.

References

  • Basnet, B., Yadav, P. K., & Devkota, A. R. (2021). Insect Pests Infestation, Diseases and Management Practice of Large Cardamom in Nepal: a Review. I TECH MAG, 3, 09–18. https://doi.org/10.26480/itechmag.03.2021.09.18
  • Belbase, S., Paudel, , Bhusal, R., Gautam, S., Aryal, A., & Kumar, S. (2018). Fungal Diseases of Large Cardamom (Amomum subulatum Roxb.) and its Integrated Management. International Journal of Current Microbiology and Applied Sciences, 7(03), 3316–3321. https://doi.org/10.20546/ijcmas.2018.703.382
  • Chaudhari, K. A., & Gohel, N. M. (2016). Management of anthracnose disease of mungbean through new fungicidal formulations. Journal of Pure and Applied Microbiology, 10(1), 691–696.
  • Devadason, A., Kuttalam, S., Ramasamy, S., Sundravadana, S., Alice, D., Kuttalam, S., & Samiyappan, R. (2016). Efficacy of azoxystrobin on Colletotrichum gloeosporiodes penz growth and on controlling mango anthracnose. Journal of Agricultural and Biological Science, 2(3), 10–15. https://www.researchgate.net/publication/237779979
  • Feksa, H. R., Do Couto, H. T. Z., Garozi, R., De Almeida, J. L., Gardiano, C. G., & Tessmann, D. J. (2019). Pre- and postinfection application of strobilurin-triazole premixes and single fungicides for control of fusarium head blight and deoxynivalenol mycotoxin in wheat. Crop Protection, 117(December 2018), 128–134. https://doi.org/10.1016/j.cropro.2018.12.003
  • Gaurilcikiene, I., & Ronis, A. (2006). The effect of strobilurin fungicides on the development of foliar diseases of winter wheat. Agronomy Research (Tartu), 4, 177–180.
  • Gautam, A. K., Sharma, D., & Avasthi, S. (2020). Management of anthracnose disease in chilli using new-generation fungicides. Journal of Mycopathological Research, 58(3), 275–280. https://doi.org/10.5958/0976-156X.2020.00036.9
  • Gautam, N., Bhattarai, R. R., Khanal, B. K. S., & Oli, P. (2016). Technology, Chemistry and Bioactive Properties of Large Cardamom (Amomum subulatum Roxb.): An Overview. International Journal of Applied Sciences and Biotechnology, 4(2), 139–149. https://doi.org/10.3126/ijasbt.v4i2.15104
  • Ghazanfar, M., Wakil, W., Sahi, S., & Kaku, K. (2009). Influence of various fungicides on the management of rice blast disease. Mycopath, 7(1), 29–34.
  • Gudade, B., Chhetri, P., Gupta, U., Deka, T. N., & Vijayan, A. K. (2013). Traditional practices of large cardamom cultivation in Sikkim and Darjeeling. Life Sciences Leaflets, 9(May), 62–68.
  • Gurung, K., & Bag, N. (2023). Leaf Blight : A major threat to large cardamom ( Amomum subulatum Roxb .) in Sikkim Himalayas. 10(05), 233–235.
  • Gurung, K., & Pandey, A. (2021). Leaf Blight Caused by Colletotrichum Fructicola of Large Cardamom (Amomum Subulatam Roxb.) an Important Cash Crop Grown in Sikkim , India. 1–21.
  • Kumar, B. (2019). Bio-Efficacy of Newer Fungicide Trifolxystrobin 25% Plus Tebuconazole 50% WG (NATIVO 75 WG) Against Antracnose Leaf Spot and Pod Blight of Soybean. The Bioscan, 14(1), 001–007.
  • Mohiddin, F. A., Bhat, N. A., Wani, S. H., Bhat, A. H., Ahanger, M. A., Shikari, A. B., Sofi, N. R., Parveen, S., Khan, G. H., Bashir, Z., Vachova, P., Hassan, S., & Sabagh, A. E. L. (2021). Combination of strobilurin and triazole chemicals for the management of blast disease in mushk budji-aromatic rice. Journal of Fungi, 7(12), 1–13. https://doi.org/10.3390/jof7121060
  • Nasir, M., Iqbal, B., Idrees, M., Sajjad, M., Niaz, M. Z., Anwar, H., Shehzad, M. A., & Tariq, A. H. (2017). Efficacy of some organic fungicides against anthracnose and powdery mildew of mango. Pakistan Journal of Agricultural Sciences, 54(3), 493–496. https://doi.org/10.21162/PAKJAS/17.1909
  • Oo, M. M., & Oh, S.-K. (2016). Chilli anthracnose (Colletotrichum spp.) disease and its management approach. Korean Journal of Agricultural Science, 43(2), 153–162. https://doi.org/10.7744/kjoas.20160018
  • Paudel, J., Belbase, S., Gautam, S., Bhusal, R., & Kumar, S. (2018). The Effect of Viral Diseases of Large Cardamom (Amomum subulatum Roxb.) on Production and their Management. International Journal of Current Microbiology and Applied Sciences, 7(03), 855–860. https://doi.org/10.20546/ijcmas.2018.703.100
  • Praveena, R., Biju, C. N., Senthilkumar, R., Darshana, C. N., & Jashmi, K. C. (2013). Preliminary evaluation of cardamom accessions against leaf blight / Chenthal disease. 66(1), 112–113.
  • Pun, A. B. (2019). A Review on Different Factors of Large Cardamom Decline in Nepal. Asian Journal of Research in Crop Science, 2(4), 1–6. https://doi.org/10.9734/ajrcs/2018/46732 Rijal, S. (2002). Impact of climate change on large cardamom-based livelihoods in panchthar district, Nepal. 33–38.
  • Saju, K. A., Mech, S., Deka, T. N., Biswas, A. K., & Sudharshan, M. . (2013). Yield loss of large cardamom ( Amomum subulatum Roxb .) due to Colletotrichum blight in Sikkim. Journal of Spices and Aromatic Crops, 22(2), 215–218.
  • Scherm, H., Christiano, R. S. C., Esker, P. D., Del Ponte, E. M., & Godoy, C. V. (2009). Quantitative review of fungicide efficacy trials for managing soybean rust in Brazil. Crop Protection, 28(9), 774–782. https://doi.org/10.1016/j.cropro.2009.05.006
  • Shrestha, J., Prasai, H. K., Timsina, K. P., Shrestha, K. P., Pokhrel, D., Poudel, K., Yadav, M. P., & (2018). Large cardamom in Nepal: Production practice and economics, Processing and Marketing (Issue February 2020).
  • Shrestha, K. P., Gopal, K. C., Chaudhary, R., Pun, A. B., Shrestha, J., Yadav, S., & Mandal, D. L. (2018). Assessment of Production Constraints of Large Cardamom in the Eastern Hills of Nepal. Asian Journal of Agricultural and Horticultural Research, 2(4), 1–10. https://doi.org/10.9734/ajahr/2018/45905
  • Singh, A. I., & Pothula, A. K. (2013). Postharvest processing of large cardamom in the eastern Himalaya. Mountain Research and Development, 33(4), 453–462. https://doi.org/10.1659/MRD-JOURNAL-D-12-00069.1
  • Singh, R., & Jadon, K. S. (2021). Fungicide resistance in phytopathogenic fungi: A major concern in disease management. Agricultural Reviews, 42(3), 246–252. https://doi.org/10.18805/ag.R-1874
  • Sony, K. C., & Upreti, B. R. (2017). The Political Economy of Cardamom Farming in Eastern Nepal: Crop Disease, Coping Strategies, and Institutional Innovation. SAGE Open, 7(2). https://doi.org/10.1177/2158244017705422
  • Subedi, A., Kattel, S., Chaudhary, L. B., & Thapa, S. B. (2022). Efficacy of bio agents and fungicides against Colletotrichum blight of large cardamom (Amomum subulatum Roxb.) under field condition in Sankhuwasabha District, Nepal. Archives of Agriculture and Environmental Science, 7(4), 529–534. https://doi.org/10.26832/24566632.2022.070408
  • Tiwari, P., Meena, R. K., & Patel, R. K. (2023). Biocontrol agents as an alternative to chemical fungicides in sustainable agriculture: A review. International Journal of Agriculture Sciences, 15(3), 155–160.
  • Vijayan, A. K. (2018). Identity of Colletotrichum infections in large cardamom ( Amomum subulatum Roxb .). Journal of Spices and Aromatic Crops, October. https://doi.org/10.13140/RG.2.2.35435.39202
  • Zubair, M., Raza, A., Ali, S., & Naeem, M. (2022). Evaluation of different fungicides against anthracnose disease caused by Colletotrichum spp. in vegetables. Plant Protection, 7(1), 50–58.
  • Zuntini, B., De Cassia Félix Alvarez, R., De Faria Theodoro, G., & Zuffo, A. M. (2019). Effect of adding fungicide to mixtures of triazoles and strobilurins in the control of downy mildew and Asian soybean rust. Pesquisa Agropecuaria Tropical, 49, 1–9. https://doi.org/10.1590/1983-40632019v4953688

Evaluation of Strobilurin and Triazole-Based Fungicides for Managing Foliar Blight in Large Cardamom in Taplejung, Nepal

Year 2025, Volume: 8 Issue: 2, 76 - 86

Abstract

Large cardamom (Amomum subulatum Roxb.) is an important spice crop in the Eastern Himalayas, including Nepal, but its productivity is severely affected by Colletotrichum Leaf Blight (CLB) caused by Colletotrichum spp. To manage this disease, a field experiment was conducted using a Randomized Complete Block Design (RCBD) with seven treatments and three replications. The treatments included three fungicides: Nativo (Tebuconazole 50% + Trifloxystrobin 25% WG), Syodia (Azoxystrobin 11% + Tebuconazole 18% SC), and Symister Top (Azoxystrobin 18.2% + Difenoconazole 11.4% SC): each applied at two different concentrations (1 g/ml per liter and a lower dose), along with a control. Before treatment, 40 leaves were randomly selected to assess initial disease severity. Fungicides were applied using a high-volume knapsack sprayer at 4 liters per plot. A second spray was done 15 days after the first when disease severity reached about 5%. All treatments followed recommended agronomic practices. Among the treatments, Symister Top at 1 ml/L was the most effective, reducing disease severity by 68.4%, followed by Nativo (61.7%) and Syodia (57.9%). The highest yield increase (22.5%) was also recorded with Symister Top. The efficacy is due to the systemic and translaminar action of the fungicides, though the risk of resistance development remains a concern. The study suggests that appropriate fungicide use can effectively manage CLB in large cardamom. However, integrating chemical control with other sustainable practices is essential for long-term disease management and environmental safety.

References

  • Basnet, B., Yadav, P. K., & Devkota, A. R. (2021). Insect Pests Infestation, Diseases and Management Practice of Large Cardamom in Nepal: a Review. I TECH MAG, 3, 09–18. https://doi.org/10.26480/itechmag.03.2021.09.18
  • Belbase, S., Paudel, , Bhusal, R., Gautam, S., Aryal, A., & Kumar, S. (2018). Fungal Diseases of Large Cardamom (Amomum subulatum Roxb.) and its Integrated Management. International Journal of Current Microbiology and Applied Sciences, 7(03), 3316–3321. https://doi.org/10.20546/ijcmas.2018.703.382
  • Chaudhari, K. A., & Gohel, N. M. (2016). Management of anthracnose disease of mungbean through new fungicidal formulations. Journal of Pure and Applied Microbiology, 10(1), 691–696.
  • Devadason, A., Kuttalam, S., Ramasamy, S., Sundravadana, S., Alice, D., Kuttalam, S., & Samiyappan, R. (2016). Efficacy of azoxystrobin on Colletotrichum gloeosporiodes penz growth and on controlling mango anthracnose. Journal of Agricultural and Biological Science, 2(3), 10–15. https://www.researchgate.net/publication/237779979
  • Feksa, H. R., Do Couto, H. T. Z., Garozi, R., De Almeida, J. L., Gardiano, C. G., & Tessmann, D. J. (2019). Pre- and postinfection application of strobilurin-triazole premixes and single fungicides for control of fusarium head blight and deoxynivalenol mycotoxin in wheat. Crop Protection, 117(December 2018), 128–134. https://doi.org/10.1016/j.cropro.2018.12.003
  • Gaurilcikiene, I., & Ronis, A. (2006). The effect of strobilurin fungicides on the development of foliar diseases of winter wheat. Agronomy Research (Tartu), 4, 177–180.
  • Gautam, A. K., Sharma, D., & Avasthi, S. (2020). Management of anthracnose disease in chilli using new-generation fungicides. Journal of Mycopathological Research, 58(3), 275–280. https://doi.org/10.5958/0976-156X.2020.00036.9
  • Gautam, N., Bhattarai, R. R., Khanal, B. K. S., & Oli, P. (2016). Technology, Chemistry and Bioactive Properties of Large Cardamom (Amomum subulatum Roxb.): An Overview. International Journal of Applied Sciences and Biotechnology, 4(2), 139–149. https://doi.org/10.3126/ijasbt.v4i2.15104
  • Ghazanfar, M., Wakil, W., Sahi, S., & Kaku, K. (2009). Influence of various fungicides on the management of rice blast disease. Mycopath, 7(1), 29–34.
  • Gudade, B., Chhetri, P., Gupta, U., Deka, T. N., & Vijayan, A. K. (2013). Traditional practices of large cardamom cultivation in Sikkim and Darjeeling. Life Sciences Leaflets, 9(May), 62–68.
  • Gurung, K., & Bag, N. (2023). Leaf Blight : A major threat to large cardamom ( Amomum subulatum Roxb .) in Sikkim Himalayas. 10(05), 233–235.
  • Gurung, K., & Pandey, A. (2021). Leaf Blight Caused by Colletotrichum Fructicola of Large Cardamom (Amomum Subulatam Roxb.) an Important Cash Crop Grown in Sikkim , India. 1–21.
  • Kumar, B. (2019). Bio-Efficacy of Newer Fungicide Trifolxystrobin 25% Plus Tebuconazole 50% WG (NATIVO 75 WG) Against Antracnose Leaf Spot and Pod Blight of Soybean. The Bioscan, 14(1), 001–007.
  • Mohiddin, F. A., Bhat, N. A., Wani, S. H., Bhat, A. H., Ahanger, M. A., Shikari, A. B., Sofi, N. R., Parveen, S., Khan, G. H., Bashir, Z., Vachova, P., Hassan, S., & Sabagh, A. E. L. (2021). Combination of strobilurin and triazole chemicals for the management of blast disease in mushk budji-aromatic rice. Journal of Fungi, 7(12), 1–13. https://doi.org/10.3390/jof7121060
  • Nasir, M., Iqbal, B., Idrees, M., Sajjad, M., Niaz, M. Z., Anwar, H., Shehzad, M. A., & Tariq, A. H. (2017). Efficacy of some organic fungicides against anthracnose and powdery mildew of mango. Pakistan Journal of Agricultural Sciences, 54(3), 493–496. https://doi.org/10.21162/PAKJAS/17.1909
  • Oo, M. M., & Oh, S.-K. (2016). Chilli anthracnose (Colletotrichum spp.) disease and its management approach. Korean Journal of Agricultural Science, 43(2), 153–162. https://doi.org/10.7744/kjoas.20160018
  • Paudel, J., Belbase, S., Gautam, S., Bhusal, R., & Kumar, S. (2018). The Effect of Viral Diseases of Large Cardamom (Amomum subulatum Roxb.) on Production and their Management. International Journal of Current Microbiology and Applied Sciences, 7(03), 855–860. https://doi.org/10.20546/ijcmas.2018.703.100
  • Praveena, R., Biju, C. N., Senthilkumar, R., Darshana, C. N., & Jashmi, K. C. (2013). Preliminary evaluation of cardamom accessions against leaf blight / Chenthal disease. 66(1), 112–113.
  • Pun, A. B. (2019). A Review on Different Factors of Large Cardamom Decline in Nepal. Asian Journal of Research in Crop Science, 2(4), 1–6. https://doi.org/10.9734/ajrcs/2018/46732 Rijal, S. (2002). Impact of climate change on large cardamom-based livelihoods in panchthar district, Nepal. 33–38.
  • Saju, K. A., Mech, S., Deka, T. N., Biswas, A. K., & Sudharshan, M. . (2013). Yield loss of large cardamom ( Amomum subulatum Roxb .) due to Colletotrichum blight in Sikkim. Journal of Spices and Aromatic Crops, 22(2), 215–218.
  • Scherm, H., Christiano, R. S. C., Esker, P. D., Del Ponte, E. M., & Godoy, C. V. (2009). Quantitative review of fungicide efficacy trials for managing soybean rust in Brazil. Crop Protection, 28(9), 774–782. https://doi.org/10.1016/j.cropro.2009.05.006
  • Shrestha, J., Prasai, H. K., Timsina, K. P., Shrestha, K. P., Pokhrel, D., Poudel, K., Yadav, M. P., & (2018). Large cardamom in Nepal: Production practice and economics, Processing and Marketing (Issue February 2020).
  • Shrestha, K. P., Gopal, K. C., Chaudhary, R., Pun, A. B., Shrestha, J., Yadav, S., & Mandal, D. L. (2018). Assessment of Production Constraints of Large Cardamom in the Eastern Hills of Nepal. Asian Journal of Agricultural and Horticultural Research, 2(4), 1–10. https://doi.org/10.9734/ajahr/2018/45905
  • Singh, A. I., & Pothula, A. K. (2013). Postharvest processing of large cardamom in the eastern Himalaya. Mountain Research and Development, 33(4), 453–462. https://doi.org/10.1659/MRD-JOURNAL-D-12-00069.1
  • Singh, R., & Jadon, K. S. (2021). Fungicide resistance in phytopathogenic fungi: A major concern in disease management. Agricultural Reviews, 42(3), 246–252. https://doi.org/10.18805/ag.R-1874
  • Sony, K. C., & Upreti, B. R. (2017). The Political Economy of Cardamom Farming in Eastern Nepal: Crop Disease, Coping Strategies, and Institutional Innovation. SAGE Open, 7(2). https://doi.org/10.1177/2158244017705422
  • Subedi, A., Kattel, S., Chaudhary, L. B., & Thapa, S. B. (2022). Efficacy of bio agents and fungicides against Colletotrichum blight of large cardamom (Amomum subulatum Roxb.) under field condition in Sankhuwasabha District, Nepal. Archives of Agriculture and Environmental Science, 7(4), 529–534. https://doi.org/10.26832/24566632.2022.070408
  • Tiwari, P., Meena, R. K., & Patel, R. K. (2023). Biocontrol agents as an alternative to chemical fungicides in sustainable agriculture: A review. International Journal of Agriculture Sciences, 15(3), 155–160.
  • Vijayan, A. K. (2018). Identity of Colletotrichum infections in large cardamom ( Amomum subulatum Roxb .). Journal of Spices and Aromatic Crops, October. https://doi.org/10.13140/RG.2.2.35435.39202
  • Zubair, M., Raza, A., Ali, S., & Naeem, M. (2022). Evaluation of different fungicides against anthracnose disease caused by Colletotrichum spp. in vegetables. Plant Protection, 7(1), 50–58.
  • Zuntini, B., De Cassia Félix Alvarez, R., De Faria Theodoro, G., & Zuffo, A. M. (2019). Effect of adding fungicide to mixtures of triazoles and strobilurins in the control of downy mildew and Asian soybean rust. Pesquisa Agropecuaria Tropical, 49, 1–9. https://doi.org/10.1590/1983-40632019v4953688
There are 31 citations in total.

Details

Primary Language English
Subjects Plant Pathology
Journal Section Research Articles
Authors

Ayush Kharel 0009-0003-1404-1233

Dipesh Mehata 0000-0002-2942-9199

Kiran Chaulagain 0009-0008-3828-9272

Pawan Kumar Yadav 0009-0007-2710-6058

Reeya Gautam 0009-0003-0405-9384

Nirmal Adhikari

Sova Yadav 0000-0003-4342-4308

Early Pub Date November 5, 2025
Publication Date November 11, 2025
Submission Date May 22, 2025
Acceptance Date September 17, 2025
Published in Issue Year 2025 Volume: 8 Issue: 2

Cite

APA Kharel, A., Mehata, D., Chaulagain, K., … Yadav, P. K. (2025). Evaluation of Strobilurin and Triazole-Based Fungicides for Managing Foliar Blight in Large Cardamom in Taplejung, Nepal. Journal of Agriculture, 8(2), 76-86. https://doi.org/10.46876/ja.1703598