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Making Butter and Milk from Plants: An Investigation into Plant-Based Butter as a Novel Approach

Year 2025, Volume: 8 Issue: 2, 87 - 102

Abstract

Plant-based milk and dairy analogues are gaining increasing attention as sustainable alternatives to animal-derived products, which are often associated with environmental and health concerns. This study aimed to develop and characterize a novel plant-based butter produced from flaxseed milk. Flaxseed was processed to obtain plant-based milk, which was subsequently used to formulate a plant-based butter. The product was evaluated in terms of sensory acceptance, pH, titratable acidity, dry matter, color parameters, and fatty acid composition. Sensory analysis indicated that flaxseed-based butter exhibited acceptable consumer attributes. Fatty acid profiling revealed nine compounds, with lauric acid (38.42%), oleic acid (18.36%), and myristic acid (15.16%) identified as the predominant fatty acids. The findings demonstrate that flaxseed-based butter has promising potential as a sustainable alternative in the category of plant-based dairy products, owing to its favorable sensory properties and suitable fatty acid profile.

Supporting Institution

TÜBİTAK Bilim İnsanı Destek Programları Başkanlığı (BİDEB)

Project Number

1919B012304651

Thanks

Yazarlar tarafından, bu çalışma TÜBİTAK Bilim İnsanı Destek Programları Başkanlığı (BİDEB) tarafından yürütülen, 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında desteklenen 1919B012304651 numaralı proje kapsamında üretilmiştir. TÜBİTAK’a destek ve katkılarından dolayı teşekkür ederiz

References

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  • Akgül, H. İ., 2015. Trabzon tereyağlarının bazı kalite parametrelerinin belirlenmesi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi. (Basılmış)
  • Altınay, C., Şanlı, T., 2023. Vegan beslenmede alternatif süt ürünleri: Teknolojik ve beslenme yönü. GIDA, 48(6), 1318–1334. https://doi.org/10.15237/gida.GD23114
  • AOAC, 2000. Fatty acids in oils and fats: Official Method 969.33 (17th ed.), Washington, DC.
  • AOAC, 1992. Official Methods of Analysis, Association of Official Analytical Chemists, Washington, DC.
  • Atamer, M., Kaptan, N., 1982. Ankara’da tüketime sunulan kahvaltılık tereyağların nitelikleri üzerine araştırmalar. Gıda, 4, 189–198.
  • Ayar, A., Özcan, M., Sert, D., Arslan, D., 2006. Yayık tereyağının raf ömrünün uzatılmasına bazı baharat, uçucu yağ ve ekstraktlarının katkısı (TÜBİTAK Proje No: TOVAG-105 O 046). Konya.
  • Aydemir, M., 2024. Evaluation of butter produced from whey and milk fat in terms of some quality criteria and fatty acid compositions. Revista Científica De La Facultad De Ciencias Veterinarias, 34(1), 1–6. https://doi.org/10.52973/rcfcv-e34293
  • Aydın, S., Kahyaoğlu, D., 2020. Antioxidant effect potential of garlic in vitro and real food system: Effects of garlic supplementation on oxidation stability and sensory properties of butter. European Journal of Lipid Science and Technology, 122(3). https://doi.org/10.1002/ejlt.201900261
  • Becskei, Z., Savić, M., Ćirković, D., Rašeta, M., Puvača, N., Pajić, M. and Paskaš, S., 2020. Assessment of water buffalo milk and traditional milk products in a sustainable production system. Sustainability, 12(16), 6616. https://doi.org/10.3390/su12166616
  • Bhatty, R.S., Cherdkiatgumchai, P., 1990. Compositional analysis of laboratory-prepared and commercial samples of linseed meal and of hulls. Journal of the American Oil Chemists’ Society, 67(2), pp. 79–84. https://doi.org/10.1007/BF02540548
  • Bilici, R., 2022. Vegan mutfağı kapsamında geliştirilmiş yenilikçi bir ürün: Bitkisel bazlı pastacı kreması. İstanbul Gelişim Üniversitesi Lisansüstü Eğitim Enstitüsü, Gastronomi, Yüksek lisans tezi.
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  • Chen, S., Bobe, G., Zimmerman, S., Hammond, E.G., Luhman, C.M., Boylston, T.D., Freeman, A.E. and Beitz, D.C. 2004. Physical and sensory properties of dairy products from cows with various milk fatty acid compositions. Journal of Agricultural and Food Chemistry, 52(10), pp. 3422–3428.
  • Chen, X., Niu H., McClements, D.J., 2024. Flaxseed oil droplets coated with potato, soy, or pea protein: Formation and stability of plant-based milk fat globules. Food Research International, 197(Part 1), 115175.
  • Costa, A., Pereira, D., Veríssimo, C., Bomfim, M., Queiroga, R., Madruga, M. and Soares, J., 2019. Developing cookies formulated with goat cream enriched with conjugated linoleic acid. PLOS ONE, 14(9), e0212534. https://doi.org/10.1371/journal.pone.0212534
  • Costa, M.A., Kuhn, D., Rama, G.R., Lehn, D.N. and Souza, C.F.V., 2022. Whey butter: A promising perspective for the dairy industry. Brazilian Journal of Food Technology, 25, e2021088. https://doi.org/10.1590/1981-6723.08821
  • Craig, W.J., 2023. Plant based dairy alternatives contribute to a healthy and nutritious diet. Nutrients, 15(15), 33. https://doi.org/10.3390/nu15153393
  • Çakmakçı, S., Kahyaoğlu, D., 2018. Farklı hayvan sütlerinden üretilen tereyağlarının depolama süresince oksidasyon stabilitesi ve bazı özellikleri üzerine karşılaştırmalı bir araştırma. Gıda / The Journal of Food, 43(2), pp. 283–293.
  • Çakmakçı, S., Çelik, K., Ertem, H., Çalışkan, H., 2020. Tereyağı olarak satılan yağlarda bazı hileler ve kalite özelliklerinin tespiti: Erzurum örneği. Gıda, 45, pp. 800–813.
  • Celik, S., Bakirci, I., 2000. A Study on the Physical and Chemical Properties of Cookery-Type Butter. Pakistan Journal of Biological Sciences, 3, 596–598.
  • Daryani, D., Pegua, K., Aryaa, S.S., 2024. Review of plant-based milk analogue: Its preparation, nutritional, physicochemical, and organoleptic properties. Food Science and Biotechnology, 33, 1059–1073. https://doi.org/10.1007/s10068-023-01482-z
  • Escobar-Sáez, D., Montero-Jiménez, L., García Herrera, P. and Sánchez-Mata, M.C., 2022. Plant based drinks for vegetarian or vegan toddlers: Nutritional evaluation of commercial products, and review of health benefits and potential concerns. Food Research International, 160, 111646. https://doi.org/10.1016/j.foodres.2022.111646
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Bitki-bazlı süt ve tereyağı üretimi: Bitki-bazlı tereyağının yenilikçi bir yaklaşım olarak incelenmesi

Year 2025, Volume: 8 Issue: 2, 87 - 102

Abstract

Plant-based milk and dairy analogues are gaining increasing attention as sustainable alternatives to animal-derived products, which are often associated with environmental and health concerns. This study aimed to develop and characterize a novel plant-based butter produced from flaxseed milk. Flaxseed was processed to obtain plant-based milk, which was subsequently used to formulate a plant-based butter. The product was evaluated in terms of sensory acceptance, pH, titratable acidity, dry matter, color parameters, and fatty acid composition. Sensory analysis indicated that flaxseed-based butter exhibited acceptable consumer attributes. Fatty acid profiling revealed nine compounds, with lauric acid (38.42%), oleic acid (18.36%), and myristic acid (15.16%) identified as the predominant fatty acids. The findings demonstrate that flaxseed-based butter has promising potential as a sustainable alternative in the category of plant-based dairy products, owing to its favorable sensory properties and suitable fatty acid profile.

Project Number

1919B012304651

References

  • Ahmed, A., Sayed, M., Kamal, S., Abdel-Hafeez, O., 2022. Physicochemical evaluation of cooking butter and hydrogenated oils. Assiut Veterinary Medical Journal, 68 (175), 106–114. https://doi.org/10.21608/avmj.2022.157084.1080
  • Akgül, H. İ., 2015. Trabzon tereyağlarının bazı kalite parametrelerinin belirlenmesi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi. (Basılmış)
  • Altınay, C., Şanlı, T., 2023. Vegan beslenmede alternatif süt ürünleri: Teknolojik ve beslenme yönü. GIDA, 48(6), 1318–1334. https://doi.org/10.15237/gida.GD23114
  • AOAC, 2000. Fatty acids in oils and fats: Official Method 969.33 (17th ed.), Washington, DC.
  • AOAC, 1992. Official Methods of Analysis, Association of Official Analytical Chemists, Washington, DC.
  • Atamer, M., Kaptan, N., 1982. Ankara’da tüketime sunulan kahvaltılık tereyağların nitelikleri üzerine araştırmalar. Gıda, 4, 189–198.
  • Ayar, A., Özcan, M., Sert, D., Arslan, D., 2006. Yayık tereyağının raf ömrünün uzatılmasına bazı baharat, uçucu yağ ve ekstraktlarının katkısı (TÜBİTAK Proje No: TOVAG-105 O 046). Konya.
  • Aydemir, M., 2024. Evaluation of butter produced from whey and milk fat in terms of some quality criteria and fatty acid compositions. Revista Científica De La Facultad De Ciencias Veterinarias, 34(1), 1–6. https://doi.org/10.52973/rcfcv-e34293
  • Aydın, S., Kahyaoğlu, D., 2020. Antioxidant effect potential of garlic in vitro and real food system: Effects of garlic supplementation on oxidation stability and sensory properties of butter. European Journal of Lipid Science and Technology, 122(3). https://doi.org/10.1002/ejlt.201900261
  • Becskei, Z., Savić, M., Ćirković, D., Rašeta, M., Puvača, N., Pajić, M. and Paskaš, S., 2020. Assessment of water buffalo milk and traditional milk products in a sustainable production system. Sustainability, 12(16), 6616. https://doi.org/10.3390/su12166616
  • Bhatty, R.S., Cherdkiatgumchai, P., 1990. Compositional analysis of laboratory-prepared and commercial samples of linseed meal and of hulls. Journal of the American Oil Chemists’ Society, 67(2), pp. 79–84. https://doi.org/10.1007/BF02540548
  • Bilici, R., 2022. Vegan mutfağı kapsamında geliştirilmiş yenilikçi bir ürün: Bitkisel bazlı pastacı kreması. İstanbul Gelişim Üniversitesi Lisansüstü Eğitim Enstitüsü, Gastronomi, Yüksek lisans tezi.
  • Bloedon, L.T., Szapary, P.O., 2004. Flaxseed and cardiovascular risk. Nutrition reviews, 62(1), pp. 18–27. https://doi.org/10.1111/j.1753-4887.2004.tb00002.x
  • Chen, S., Bobe, G., Zimmerman, S., Hammond, E.G., Luhman, C.M., Boylston, T.D., Freeman, A.E. and Beitz, D.C. 2004. Physical and sensory properties of dairy products from cows with various milk fatty acid compositions. Journal of Agricultural and Food Chemistry, 52(10), pp. 3422–3428.
  • Chen, X., Niu H., McClements, D.J., 2024. Flaxseed oil droplets coated with potato, soy, or pea protein: Formation and stability of plant-based milk fat globules. Food Research International, 197(Part 1), 115175.
  • Costa, A., Pereira, D., Veríssimo, C., Bomfim, M., Queiroga, R., Madruga, M. and Soares, J., 2019. Developing cookies formulated with goat cream enriched with conjugated linoleic acid. PLOS ONE, 14(9), e0212534. https://doi.org/10.1371/journal.pone.0212534
  • Costa, M.A., Kuhn, D., Rama, G.R., Lehn, D.N. and Souza, C.F.V., 2022. Whey butter: A promising perspective for the dairy industry. Brazilian Journal of Food Technology, 25, e2021088. https://doi.org/10.1590/1981-6723.08821
  • Craig, W.J., 2023. Plant based dairy alternatives contribute to a healthy and nutritious diet. Nutrients, 15(15), 33. https://doi.org/10.3390/nu15153393
  • Çakmakçı, S., Kahyaoğlu, D., 2018. Farklı hayvan sütlerinden üretilen tereyağlarının depolama süresince oksidasyon stabilitesi ve bazı özellikleri üzerine karşılaştırmalı bir araştırma. Gıda / The Journal of Food, 43(2), pp. 283–293.
  • Çakmakçı, S., Çelik, K., Ertem, H., Çalışkan, H., 2020. Tereyağı olarak satılan yağlarda bazı hileler ve kalite özelliklerinin tespiti: Erzurum örneği. Gıda, 45, pp. 800–813.
  • Celik, S., Bakirci, I., 2000. A Study on the Physical and Chemical Properties of Cookery-Type Butter. Pakistan Journal of Biological Sciences, 3, 596–598.
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There are 71 citations in total.

Details

Primary Language English
Subjects Food Sustainability, Food Technology
Journal Section Research Articles
Authors

Ayşe Burcu Atalay 0000-0002-2882-7865

İrem Algül This is me 0009-0008-8740-1574

Project Number 1919B012304651
Early Pub Date November 6, 2025
Publication Date November 10, 2025
Submission Date June 3, 2025
Acceptance Date September 15, 2025
Published in Issue Year 2025 Volume: 8 Issue: 2

Cite

APA Atalay, A. B., & Algül, İ. (2025). Making Butter and Milk from Plants: An Investigation into Plant-Based Butter as a Novel Approach. Journal of Agriculture, 8(2), 87-102. https://doi.org/10.46876/ja.1713087