Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30˚C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied
Sıvakumar, N., Remya, R., & Saıf, A. B. (2009). Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences, 3(3), 71-75.
AMA
Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. November 2009;3(3):71-75.
Chicago
Sıvakumar, Nallusamy, Rajendran Remya, and Al Bahry Saıf. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3, no. 3 (November 2009): 71-75.
EndNote
Sıvakumar N, Remya R, Saıf AB (November 1, 2009) Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences 3 3 71–75.
IEEE
N. Sıvakumar, R. Remya, and A. B. Saıf, “Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii”, J.appl.biol.sci., vol. 3, no. 3, pp. 71–75, 2009.
ISNAD
Sıvakumar, Nallusamy et al. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3/3 (November 2009), 71-75.
JAMA
Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3:71–75.
MLA
Sıvakumar, Nallusamy et al. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences, vol. 3, no. 3, 2009, pp. 71-75.
Vancouver
Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3(3):71-5.
