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Year 2009, Volume: 3 Issue: 3, 71 - 75, 01.11.2009

Nallusamy Sıvakumar Rajendran Remya Al Bahry Saıf

Abstract

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Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii

Year 2009, Volume: 3 Issue: 3, 71 - 75, 01.11.2009

Nallusamy Sıvakumar Rajendran Remya Al Bahry Saıf

Abstract

Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30˚C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied

Keywords

Destaining activity Dioscorea wallichii protease zymogram

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Details

Other ID JA39KE57JC
Journal Section Research Article
Authors

Nallusamy Sıvakumar This is me

Rajendran Remya This is me

Al Bahry Saıf This is me

Publication Date November 1, 2009
Published in Issue Year 2009 Volume: 3 Issue: 3

Cite

APA Sıvakumar, N., Remya, R., & Saıf, A. B. (2009). Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences, 3(3), 71-75.
AMA Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. November 2009;3(3):71-75.
Chicago Sıvakumar, Nallusamy, Rajendran Remya, and Al Bahry Saıf. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3, no. 3 (November 2009): 71-75.
EndNote Sıvakumar N, Remya R, Saıf AB (November 1, 2009) Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences 3 3 71–75.
IEEE N. Sıvakumar, R. Remya, and A. B. Saıf, “Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii”, J.appl.biol.sci., vol. 3, no. 3, pp. 71–75, 2009.
ISNAD Sıvakumar, Nallusamy et al. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3/3 (November 2009), 71-75.
JAMA Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3:71–75.
MLA Sıvakumar, Nallusamy et al. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences, vol. 3, no. 3, 2009, pp. 71-75.
Vancouver Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3(3):71-5.