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Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts

Year 2010, Volume: 4 Issue: 1, 7 - 16, 01.02.2010

Kaouthar Boudıaf Zahira Houcher Widad Sobhı Mustapha Benboubetra

Abstract

Nigella sativa , one of the most studied medicinal plants over the world, possesses a number of well established pharmacological properties. In this study, we have prepared different extracts from Nigella sativa’s seeds. The results show that hexane (HxE), chloroform (ChE) and ethyl acetate (AcE) extracts present an apparent inhibitory effect on both xanthine oxidase (XO) and xanthine dehydrogenase (XDH) activities of the enzyme xanthine oxidoreductase (XOR), with a markedly better efficiency for XDH activity.Scavenging power on radical anion superoxide (O2•¯) by different N. sativa extracts has been evaluated using chemiluminescence assay where two extracts, HxE and ChE, exhibited good scavenger activity, with IC50 of 0.18 µg/ml and 0.3 µg/ml, respectively. Reducing power (RP) of the different obtained extracts has been assessed by the FRAP assay. ChE and AcE showed the highest RP, which is proportional to the polyphenol content. The obtained data revealed that the antioxidant activity of Nigella sativa’s seeds resides in the oil fraction of HxE (inhibition of XO and XDH, and scavenging O2•¯) and in ChE and AcE fractions expressing the most important reducing power

Keywords

Antioxidants Reactive oxygen species (ROS) Nigella sativa Reducing power (RP) Xanthine oxidoreductase (XOR)

Year 2010, Volume: 4 Issue: 1, 7 - 16, 01.02.2010

Kaouthar Boudıaf Zahira Houcher Widad Sobhı Mustapha Benboubetra

Abstract

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Details

Other ID JA69RJ47DU
Journal Section Research Article
Authors

Kaouthar Boudıaf This is me

Zahira Houcher This is me

Widad Sobhı This is me

Mustapha Benboubetra This is me

Publication Date February 1, 2010
Published in Issue Year 2010 Volume: 4 Issue: 1

Cite

APA Boudıaf, K., Houcher, Z., Sobhı, W., Benboubetra, M. (2010). Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts. Journal of Applied Biological Sciences, 4(1), 7-16.
AMA Boudıaf K, Houcher Z, Sobhı W, Benboubetra M. Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts. J.appl.biol.sci. February 2010;4(1):7-16.
Chicago Boudıaf, Kaouthar, Zahira Houcher, Widad Sobhı, and Mustapha Benboubetra. “Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella Sativa Linn seeds’ Extracts”. Journal of Applied Biological Sciences 4, no. 1 (February 2010): 7-16.
EndNote Boudıaf K, Houcher Z, Sobhı W, Benboubetra M (February 1, 2010) Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts. Journal of Applied Biological Sciences 4 1 7–16.
IEEE K. Boudıaf, Z. Houcher, W. Sobhı, and M. Benboubetra, “Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts”, J.appl.biol.sci., vol. 4, no. 1, pp. 7–16, 2010.
ISNAD Boudıaf, Kaouthar et al. “Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella Sativa Linn seeds’ Extracts”. Journal of Applied Biological Sciences 4/1 (February 2010), 7-16.
JAMA Boudıaf K, Houcher Z, Sobhı W, Benboubetra M. Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts. J.appl.biol.sci. 2010;4:7–16.
MLA Boudıaf, Kaouthar et al. “Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella Sativa Linn seeds’ Extracts”. Journal of Applied Biological Sciences, vol. 4, no. 1, 2010, pp. 7-16.
Vancouver Boudıaf K, Houcher Z, Sobhı W, Benboubetra M. Evaluation of Antioxidant and Anti-Xanthine Oxidoreductase Activities of Nigella sativa Linn seeds’ extracts. J.appl.biol.sci. 2010;4(1):7-16.