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Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii

Year 2011, Volume: 5 Issue: 1, 7 - 10, 01.02.2011

Muthuvel Arumugam Thangappan Ajıthkumar Thangavel Balasubramanıan

Abstract

Type V like collagens is widely distributed in marine invertebrates, particularly crustaceans and molluscs. The present study the collagen was prepared from the outer skin of L. uyii. The net yield of L. uyii acid soluble collagen estimated as 10.54% (ASC), subsequent digestion of residue with pepsin soluble collagen was yielded as 31.16% (PSC). SDS-PAGE analysis was revealed the chain composition of (α1)2α2 heterotrimer for ASC which similar to Japanese common squid as well as cuttle fish collagen. The ASC and PSC total protein was determined by Bradford method which contains 64.27µg/ml and 18.93µg/ml respectively. Fourier transforms infrared spectroscopy Analysis (FT-IR) of ASC and PSC is helpful in predication and confirmation of secondary structure of proteins. This report indicates that L uyii outer skin has potential to supplementing the skin of land vertebrates as a source of collagen

Keywords

collagen mollusk FT-IR SDS-PAGE squid

Year 2011, Volume: 5 Issue: 1, 7 - 10, 01.02.2011

Muthuvel Arumugam Thangappan Ajıthkumar Thangavel Balasubramanıan

Abstract

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Details

Other ID JA76HD33FM
Journal Section Research Article
Authors

Muthuvel Arumugam This is me

Thangappan Ajıthkumar This is me

Thangavel Balasubramanıan This is me

Publication Date February 1, 2011
Published in Issue Year 2011 Volume: 5 Issue: 1

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APA Arumugam, M., Ajıthkumar, T., & Balasubramanıan, T. (2011). Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences, 5(1), 7-10.
AMA Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. February 2011;5(1):7-10.
Chicago Arumugam, Muthuvel, Thangappan Ajıthkumar, and Thangavel Balasubramanıan. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5, no. 1 (February 2011): 7-10.
EndNote Arumugam M, Ajıthkumar T, Balasubramanıan T (February 1, 2011) Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences 5 1 7–10.
IEEE M. Arumugam, T. Ajıthkumar, and T. Balasubramanıan, “Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii”, J.appl.biol.sci., vol. 5, no. 1, pp. 7–10, 2011.
ISNAD Arumugam, Muthuvel et al. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5/1 (February 2011), 7-10.
JAMA Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5:7–10.
MLA Arumugam, Muthuvel et al. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences, vol. 5, no. 1, 2011, pp. 7-10.
Vancouver Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5(1):7-10.