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Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients

Year 2016, Volume: 10 Issue: 2, 51 - 55, 01.07.2016

Abstract

Scleroderma is a rarely seen chronic collagen tissue disease which has been first described in the 18th century. It is indicated that the mutations in BMPR2 (bone morphogenetic protein receptor type II) gene affected the course of disease.In this study; determination of BMPR2 gene expression levels via Real Time PCR and its association with the clinical course in scleroderma patients were revealed.20 Scleroderma diagnosed and 29 healthy individuals were taken into this study. Performing the transformation of cDNA complementary to mRNA isolated from leucocyte cells, DNA amplification was carried out via Real-Time PCR. To determine the relative expression level of BMPR2 gene via Real-Time PCR, Beta actin gene was selected as the reference gene.Mean expression levels of BMPR2 gene in patient group showed significant decrease in comparison with control group (Cp<0,05). Expression levels of patients decreased around 39,46% in comparison with control group. 4 of these 20 patients included into the study diagnosed with PAH (Pulmonary Arterial Hypertension) and gene expression levels of these patients were seen to increase.Normalized data was analyzed by using mixed model and statistically different groups were determined by Minimal Important Difference (MID) test.Consequently, in Scleroderma patients BMPR2 gene expression level showed changes from person to person but it decreased by comparison to healthy control group and in patients withPAH that was foundto be higher than the gene expression levels of other patient group

Year 2016, Volume: 10 Issue: 2, 51 - 55, 01.07.2016

Abstract

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Details

Other ID JA49BP36YE
Journal Section Research Article
Authors

Yasemin Koç This is me

Deniz Tanrısever This is me

Seçil Berna Kuzu This is me

Akın Yığın This is me

Hatice Korkmaz Güvenmez This is me

Publication Date July 1, 2016
Published in Issue Year 2016 Volume: 10 Issue: 2

Cite

APA Koç, Y., Tanrısever, D., Kuzu, S. B., Yığın, A., et al. (2016). Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients. Journal of Applied Biological Sciences, 10(2), 51-55.
AMA Koç Y, Tanrısever D, Kuzu SB, Yığın A, Güvenmez HK. Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients. J.appl.biol.sci. July 2016;10(2):51-55.
Chicago Koç, Yasemin, Deniz Tanrısever, Seçil Berna Kuzu, Akın Yığın, and Hatice Korkmaz Güvenmez. “Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and Its Association With the Clinical Course of Patients”. Journal of Applied Biological Sciences 10, no. 2 (July 2016): 51-55.
EndNote Koç Y, Tanrısever D, Kuzu SB, Yığın A, Güvenmez HK (July 1, 2016) Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients. Journal of Applied Biological Sciences 10 2 51–55.
IEEE Y. Koç, D. Tanrısever, S. B. Kuzu, A. Yığın, and H. K. Güvenmez, “Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients”, J.appl.biol.sci., vol. 10, no. 2, pp. 51–55, 2016.
ISNAD Koç, Yasemin et al. “Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and Its Association With the Clinical Course of Patients”. Journal of Applied Biological Sciences 10/2 (July 2016), 51-55.
JAMA Koç Y, Tanrısever D, Kuzu SB, Yığın A, Güvenmez HK. Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients. J.appl.biol.sci. 2016;10:51–55.
MLA Koç, Yasemin et al. “Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and Its Association With the Clinical Course of Patients”. Journal of Applied Biological Sciences, vol. 10, no. 2, 2016, pp. 51-55.
Vancouver Koç Y, Tanrısever D, Kuzu SB, Yığın A, Güvenmez HK. Determination of BMPR2 Expression in Scleroderma Patients Via Real-Time PCR and its Association with the Clinical Course of Patients. J.appl.biol.sci. 2016;10(2):51-5.