Year 2021, Volume 5 , Issue 1, Pages 65 - 73 2021-03-31

Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives

Aylin YILMAZ ÇETİNKAYA [1] , Selçuk YURTSEVER [2]


In the present study, the effects of several food colorings, namely (Ponceau 4R (E 124), Tartrazine (E 102), and Pea green (E 102-E 133), were investigated in vivo using the wing spot test, SMART (somatic mutation and recombination test), in Drosophila melanogaster. Food colorings are the food additives, which are used for improving the appearance of food and beverages. In SMART, multiple wing hair (mwh), flare (flr3), and beaded serrate (BdS) marker genes on the third-largest chromosome of Drosophila are used. The genotoxic effects of the food colorings on the imaginal disc cells that will develop into the wing spot cells during the embryonic development of Drosophila heterozygous larvae and the genotypic changes caused by mutation or recombination in somatic cells also play a role in the formation of mutant spots in the wings. Classes by mutant clones are as follows: small single spots containing 1-2 mwh, large single spots containing ≥3 mwh or ≥4 flr3, and twin spots containing adjacent mwh and flr3 cells (GRAF et al., 1984). Negative control medium was prepared with distilled water, while positive control medium was prepared with 1 mM EMS (ethyl methane sulfonate). According to results obtained from SMART, Ponceau 4R, Tartrazine, and Pea green demonstrated significant results in trans-heterozygous flies (mwh/flr3) for inducing the mutant wing spots compared to control groups at 25 mg/ml, 50 mg/ml, and 75 mg/ml exposure concentrations. On the other hand, Ponceau 4R, Tartrazine, and Pea green yielded significant results for inducing the mutant wing spots in balancer-heterozygous flies (mwh/TM3) at 25 mg/ml, 50 mg/ml, and 75 mg/ml exposure concentrations. The numbers of mutant wing spots were increased by all three colorings depending on the concentration (X2= df=3, P<0.001). It was also determined that these numbers were significantly higher than the flies in the negative control medium and it suggests that these food colorings have genotoxic effects. However, the numbers of mutant wing spot were less than the flies in the positive control medium; this finding indicates that genotoxic effects of the food colorings were not as much as the EMS.
Ponceau 4R, Tartazine, Pea green, Drosophila melanogaster Meigen, SMART
  • Akbulut, M., (2011). Food additives: functions and resources. 1.Ulusal Helal ve Sağlıklı Gıda Kongresi, Kasım 19–20, 2011, Ankara, Türkiye, (in Turkish).
  • Alkan, F. U., & Anlas, C. (2015). Genotoxicity tests and genotoxic poisons. Turkish Clinics J Vet Sci Pharmacol Toxicol-Special Topics, 1, 69-74.
  • Bernards, A., & Hariharan, I. K. (2001). Of flies and men studying human disease in Drosophila. Current opinion in genetics & development, 11(3), 274-278. Doi: https://doi.org/10.1016/S0959-437X(00)00190-8
  • Garcia-Bellido, A., & Dapena, J. (1974). Induction, detection and characterization of cell differentiation mutants in Drosophila. Molecular and General Genetics MGG, 128(2), 117-130. Doi: https://doi.org/10.1007/BF02654485
  • Graf, U., Würgler, F. E., Katz, A. J., Frei, H., Juon, H., Hall, C. B., & Kale, P. G. (1984). Somatic mutation and recombination test in Drosophila melanogaster. Environmental mutagenesis, 6(2), 153-188. https://doi.org/10.1002/em.2860060206
  • Graf, U., & Würgler, F. E. (1996). The somatic white-ivory eye spot test does not detect the same spectrum of genotoxic events as the wing somatic mutation and recombination test in Drosophila melanogaster. Environmental and molecular mutagenesis, 27(3), 219-226. Doi: https://doi.org/10.1002/(sici)1098-2280(1996)27:3%3C219::aid-em7%3E3.0.co;2-9
  • Kasımoğlu, C , Uysal, H . (2016). Genoprotective Effects of Aqueous Extracts of Rosa Canina L. Fruits on Ethyl Methanesulfonate-Induced DNA Damage in Drosophila Melanogaster . Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi , 37 (3) , 241-247 . Doi: https://doi.org/10.17776/csj.18487
  • Kaya, B. (2000). Investigation of mutagenic and recombinogenic effects of some pesticides on Drosophila melanogaster lines. Akdeniz Üniversitesi Fen Bilimleri Enstitüsü Yayınları, Antalya, Türkiye, (in Turkish).
  • Kaya, B., Yanikoglu, A., & Marcos, R. (1999). Genotoxicity studies on the phenoxyacetates 2, 4-D and 4-CPA in the Drosophila wing spot test. Teratogenesis, carcinogenesis, and mutagenesis, 19(4), 305-312. Doi: https://doi.org/10.1002/(SICI)1520-6866(1999)19:4%3C305::AID-TCM7%3E3.0.CO;2-X
  • Kumar, P.B., Moses, S., Arunkumar, B., Ramamurthy, D.S. (2020). Effect of Developmental Toxicity in Drosophila melanogaster on Exposure to Different Food Dyes (Brilliant Blue and Sunset Yellow). International Journal of Science and Research, 9 (12), 637–641. doi: https://doi.org/10.21275/SR201212195534
  • Lindsley, D.L. & Grell, E.H., (1968): Genetic variations of Drosophila melanogaster. Carnegie Institutution of Washington Publ., Washington DC, 627, p 472. Doi: https://doi.org/10.1126/science.162.3857.993
  • Lindsley, D.L. & Zimm, G.G., (1992): The genome of Drosophila melanogaster. Academic Press, San Diego, CA, p 1133.
  • Maier, E., Kurz, K., Jenny, M., Schennach, H., Ueberall, F., & Fuchs, D. (2010). Food preservatives sodium benzoate and propionic acid and colorant curcumin suppress Th1-type immune response in vitro. Food and chemical toxicology, 48(7), 1950-1956. Doi: https://doi.org/10.1016/j.fct.2010.04.042
  • Mpountoukas, P., Pantazaki, A., Kostareli, E., Christodoulou, P., Poliliou, S., Mourelatos, C., Lambropoulou, V., Lialiaris, T. (2010). Cytogenetic evaluation and DNA interaction studies of the food colourants amaranth, erythrosine and tartrazine. Food Chem. Toxicol., 48, 2934–2944. Doi: https://doi.org/10.1016/j.fct.2010.07.030
  • Tripathy, N.K., Patnaik, K.K., & Nabi, M.J. (1989). Genotoxicity of tartrazine studied in two somatic assays of Drosophila melanogaster. Mutation Research/Genetic Toxicology, 224(4), 479-483. Doi: https://doi.org/10.1016/0165-1218(89)90073-6
  • Patterson, R.M., & Butler, J.S. (1982). Tartrazine-induced chromosomal aberrations in mammalian cells. Food and Chemical Toxicology, 20(4), 461-465. Doi: https://doi.org/10.1016/s0278-6915(82)80113-0
  • Poul, M., Jarry, G., Elhkim, M.O., & Poul, J.M. (2009). Lack of genotoxic effect of food dyes amaranth, sunset yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. Food and chemical toxicology, 47(2), 443-448. Doi: https://doi.org/10.1016/j.fct.2008.11.034
  • Sarıkaya, R , Selvi, M , Akkaya, N , Acar, M , Erkoç, F. (2010). Farklı Konsantrasyonlardaki Gıda Boyalarının Drosophila melanogaster (mwh x flr)’de Yaşama Yüzdesi Üzerine Etkisi . Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi , 5 (1) , 38-46, (in Turkish).
  • Sasaki, Y.F., Kawaguchi S., Kamaya A., Ohshita M., Kabasawa K., Iwama K., Taniguchi K., Tsuda S., (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat. Res.-Genet. Toxicol. Environmental Mutagenesis, 519 (1-2), 103–119. Doi: https://doi.org/10.1016/s1383-5718(02)00128-6
  • Semerdoken, S., (2012). Effects of some synthetic azo food dyes added to foodstuffs on larval mortality and longevity of Drosophila melanogaster’s oregon R wild and vestigial mutant strains. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Yayınları, Erzurum, Türkiye, (in Turkish).
  • Topsoy, H., (1990). Quantification of synthetic paints added to some sugary foods. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü Yayınları, Ankara, Türkiye, (in Turkish).
  • Turkoglu, S., Benli, D., Sahin, D., ( 2015). The effects of five food dyes on the longevity of Drosophila melanogaster. Fresenius Environmental Bulletin, 24(9), 2830–2836.
  • Uysal, H., Genç, S., & Ayar, A. (2017). Toxic effects of chronic feeding with food azo dyes on Drosophila melanogaster Oregon R. Scientia Iranica, 24(6), 3081-3086. Doi: https://dx.doi.org/10.24200/sci.2017.4523
  • Uysal, H , Semerdöken, S. (2011). Sentetik Gıda Boyalarının Drosophila melanogaster’in Oregon R Soyunda Larval Toksisite ve Ergin Ömür Uzunluğu Üzerine Etkilerinin Belirlenmesi. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi , 4 (1) , 71-87, (in Turkish).
  • Vural, N. (2005). General Toxicology. Anadolu Üniversitesi Eczacılık Fakültesi Yayınları, Eskişehir, Türkiye, s 48. (in Turkish).
  • Yaman, M. (1996). Investigation of synthetic dye amount added to some foodstuffs. Gazi Üniversitesi Sosyal Bilimleri Enstitüsü Yayınları, Ankara, Türkiye, (in Turkish).
  • Yentür, G. (1996). Ankara piyasasindan sağlanan pasta süsleri ve bazi şekerlerde sentetik boya miktarlarinin araştirilmasi. Ankara Üniversitesi Veteriner Fakültesi Dergisi , 43 (04) , 479-484, (in Turkish).
  • Zordan, M., Osti, M., Pesce, M., & Costa, R. (1994). Chloral hydrate is recombinogenic in the wing spot test in Drosophila melanogaster. Mutation Research/Genetic Toxicology, 322(2), 111-116. Doi: https://doi.org/10.1016/0165-1218(94)00017-4
Primary Language en
Subjects Food Science and Technology
Published Date March 2021
Journal Section Research Articles
Authors

Orcid: 0000-0001-6807-9016
Author: Aylin YILMAZ ÇETİNKAYA (Primary Author)
Institution: TRAKYA ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-0678-7495
Author: Selçuk YURTSEVER
Institution: TRAKYA ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : November 25, 2020
Acceptance Date : January 25, 2021
Publication Date : March 31, 2021

APA Yılmaz Çetinkaya, A , Yurtsever, S . (2021). Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives . International Journal of Agriculture Environment and Food Sciences , 5 (1) , 65-73 . DOI: 10.31015/jaefs.2021.1.9