While the cultivation of modern biotechnology products was adopted by producers very fast, the consumer acceptance of these products has not evolved at the same pace. Despite multiple benefits associated with genetically modified organisms (GMO), some potential risks related to the environment, health and economy have increased concerns and affected consumer confidence. This article examines the relationship between knowledge and confidence levels for biosafety and GMOs, and the sociodemographic characteristics of professionals working in the fields of food, agriculture and veterinary medicine, with a view to assess their perception. Questionnaires were applied to 261 individuals, selected by the proportional sampling method among professionals, the numbers of whom were obtained from the relevant unions and chambers. Then frequency, percentage distribution and factor analyses were carried out on data obtained. In conclusion food engineering professionals, men and more knowledgeable participants in biotechnology were found to have a more positive approach towards GMOs and more confidence in the biosafety system. It is indisputable that the scientific knowledge is essential for a reliable biosafety mechanism. Therefore, the inclusion of courses on GMOs and biosafety into curricula and planning in-service trainings may significantly contribute to the development of scientific knowledge thus, improving attitudes towards GMOs.