Validation Methods for Phenolic Components with RP-HPLC-UV in Various Bee Products

Year 2018, Volume: 1 Issue: 1, 13 - 19, 01.04.2018

Hilal Ebru Çakır Yakup Şirin Sevgi Kolaylı Zehra Can

Abstract

In this study, a simple and a fast method were
developed using RP-HPLC-UV for the separation and quantitative determination of
phenolic compounds in various bee products. Fourteen phenolic compounds were
identified using 70% acetonitrile in water, and 2% acetic acid in water as a
mobile phase with a gradient elution mode. The validation method exhibited
linearity (R²>0.994), limits of detection (0.022-0.062 mg/L) and
quantification (0.030-0.187mg/L). Under the optimized conditions, 14
commercially available phenolic compounds were analyzed in less than 50 min.
This method was successfully employed to study the phenolic profiles of bee
products as well as other natural samples.    

Keywords

RP-HPLC-UV; phenolic compounds; bee products; quantification; validation

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