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Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells

Year 2018, Volume: 1 Issue: 3 - 2nd Propolis Conference Special Issue, 45 - 45, 08.12.2018

Y. Frıón-herrera D. Gabbıa M. Carrara

Abstract






Several studies
have confirmed that propolis mono-therapy can inhibit the growth of different
carcinomas cells1 due to its chemical composition2. At
present, there are few studies about the possible use of this natural product
in the treatment of chemoresistant tumors3. Combination
experiments were carried out in order to study the ability of Cuban propolis
extracts (CP) and nemorosone, its main phytocomponent, to increase of
antiproliferative efficacy of the traditional cytotoxic chemotherapic drugs
doxorubicin (Dox) and oxaliplatin (Oxa) in human colon carcinoma cells
(LoVo), doxorubicin-resistant cells (LoVo Dox) and oxaliplatin-resistant
cells (LoVo Oxa). The effects of combination therapy were investigated in
vitro evaluating cell viability at 24, 48 and 72 hs. Synergism/additivity/antagonism
effect was assessed by isobologram/combination index analysis. Molecular
mechanism of combination treatment was examined assessing LDH release,
Annexin/PI assay, ROS production, mitochondrial membrane potential (ΔΨm)
disturbance and ATP intracellular levels. Meanwhile, the expression of genes
related to apoptosis P53, BAX and BCL2 were analyzed by qRT-PCR. Our result
revealed that combination treatment resulted in a significantly synergistic
antiproliferative and cytotoxic effect at 72 hs with respect to monotherapy,
in particular in drug-resistant cell lines. Furthermore, combination
treatment induced apoptosis through activation of pro-apoptotic genes (P53
and BAX) and downregulation of BCL2 with marked disruption of ΔΨm, decrease
in ATP production and induction of ROS. These findings indicate that
combination of CP or nemorosone with classical chemotherapic drugs may
represent an intriguing treatment option for drug-resistant human colon
carcinoma.


Keywords

Synergisitic Cytotoxic Effect Cuban Propolis

References

  • 1. Oršolić N. A review of propolis antitumour action in vivo and in vitro. Journal of ApiProduct and ApiMedical Science. 2010; 2 (1):1 - 20.
  • 2. Marcucci M. Propolis: chemical composition, biological properties and therapeutic activity. Apidologie. 1995;26:83-99.
  • 3. Patel S. Emerging Adjuvant Therapy for Cancer: Propolis and its Constituents. Journal of dietary supplements. 2016;13(3):245

Year 2018, Volume: 1 Issue: 3 - 2nd Propolis Conference Special Issue, 45 - 45, 08.12.2018

Y. Frıón-herrera D. Gabbıa M. Carrara

Abstract

References

  • 1. Oršolić N. A review of propolis antitumour action in vivo and in vitro. Journal of ApiProduct and ApiMedical Science. 2010; 2 (1):1 - 20.
  • 2. Marcucci M. Propolis: chemical composition, biological properties and therapeutic activity. Apidologie. 1995;26:83-99.
  • 3. Patel S. Emerging Adjuvant Therapy for Cancer: Propolis and its Constituents. Journal of dietary supplements. 2016;13(3):245
There are 3 citations in total.

Details

Primary Language English
Journal Section 2nd Propolis Conference Special Issue
Authors

Y. Frıón-herrera This is me

D. Gabbıa This is me

M. Carrara This is me

Publication Date December 8, 2018
Published in Issue Year 2018 Volume: 1 Issue: 3 - 2nd Propolis Conference Special Issue

Cite

APA Frıón-herrera, Y., Gabbıa, D., & Carrara, M. (2018). Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells. Journal of Apitherapy and Nature, 1(3), 45-45.
AMA Frıón-herrera Y, Gabbıa D, Carrara M. Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells. J.Apit.Nat. December 2018;1(3):45-45.
Chicago Frıón-herrera, Y., D. Gabbıa, and M. Carrara. “Combination Treatment of Cuban Propolis and Nemorosone With Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-Resistant Human Colon Carcinoma Cells”. Journal of Apitherapy and Nature 1, no. 3 (December 2018): 45-45.
EndNote Frıón-herrera Y, Gabbıa D, Carrara M (December 1, 2018) Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells. Journal of Apitherapy and Nature 1 3 45–45.
IEEE Y. Frıón-herrera, D. Gabbıa, and M. Carrara, “Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells”, J.Apit.Nat., vol. 1, no. 3, pp. 45–45, 2018.
ISNAD Frıón-herrera, Y. et al. “Combination Treatment of Cuban Propolis and Nemorosone With Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-Resistant Human Colon Carcinoma Cells”. Journal of Apitherapy and Nature 1/3 (December 2018), 45-45.
JAMA Frıón-herrera Y, Gabbıa D, Carrara M. Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells. J.Apit.Nat. 2018;1:45–45.
MLA Frıón-herrera, Y. et al. “Combination Treatment of Cuban Propolis and Nemorosone With Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-Resistant Human Colon Carcinoma Cells”. Journal of Apitherapy and Nature, vol. 1, no. 3, 2018, pp. 45-45.
Vancouver Frıón-herrera Y, Gabbıa D, Carrara M. Combination Treatment of Cuban Propolis and Nemorosone with Chemotherapeutic Agents Induce a Synergisitic Cytotoxic Effect in Drug-resistant Human Colon Carcinoma Cells. J.Apit.Nat. 2018;1(3):45-.
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