Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Year 2019, Volume: 2 Issue: 2, 30 - 45, 23.04.2020

Ayşegül Özer Erva Rakıcı Kadriye Inan Bektas Sabriye Çanakçı , Ali Osman Belduz

https://doi.org/10.35206/jan.554729

Cited By: 3

Abstract

Nine
lignin-degrading bacteria were isolated from petroleum-contaminated soil and animal
manure samples and characterized by 16S rRNA sequence analysis. Three isolates
were identified as Enterobacter cancerogenus, two as Enterobacter
ludwigii, one as Citrobacter sedlakii,
one as Citrobacter farmeri, one as Klebsiella pneumoniae, and one as Citrobacter murliniae.
These  bacteria used ligno sulphate as
the sole carbon source but  did not
utilize kraft lignin (KL) as the sole source of carbon and energy. For this reason, basic nutrients, such as 1.0% glucose
(w/v) and 0.5% peptone (w/v), were used as additional carbon and nitrogen
sources to stimulate bacterial growth for KL decolorization.
Under these conditions, the isolates L1, L2, L3, L4, PT21, PT22, PT41, G1, and
C1 degraded kraft lignin by 37 %, 14 %, 20%, 43%, 48%, 51%, 28%, 60%, and %99,
respectively. The decolorization of Remazol Brilliant Blue R (RBBR) by the
isolates was analyzed. The isolates were decolorized at 20-90 % of RBBR,
respectively. 

Keywords

Lignin, Bacteria, 16S rRNA

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