Year 2019, Volume 2 , Issue 2, Pages 30 - 45 2020-04-23

Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Ayşegül ÖZER [1] , Erva RAKICI [2] , Kadriye INAN BEKTAS [3] , Sabriye ÇANAKÇI [4] , Ali Osman BELDUZ [5]


Nine lignin-degrading bacteria were isolated from petroleum-contaminated soil and animal manure samples and characterized by 16S rRNA sequence analysis. Three isolates were identified as Enterobacter cancerogenus, two as Enterobacter ludwigii, one as Citrobacter sedlakii, one as Citrobacter farmeri, one as Klebsiella pneumoniae, and one as Citrobacter murliniae. These  bacteria used ligno sulphate as the sole carbon source but  did not utilize kraft lignin (KL) as the sole source of carbon and energy. For this reason, basic nutrients, such as 1.0% glucose (w/v) and 0.5% peptone (w/v), were used as additional carbon and nitrogen sources to stimulate bacterial growth for KL decolorization. Under these conditions, the isolates L1, L2, L3, L4, PT21, PT22, PT41, G1, and C1 degraded kraft lignin by 37 %, 14 %, 20%, 43%, 48%, 51%, 28%, 60%, and %99, respectively. The decolorization of Remazol Brilliant Blue R (RBBR) by the isolates was analyzed. The isolates were decolorized at 20-90 % of RBBR, respectively. 

Lignin, Bacteria, 16S rRNA
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Primary Language en
Subjects Basic Sciences
Journal Section Research Articles
Authors

Author: Ayşegül ÖZER
Institution: Karadeniz Technical University
Country: Turkey


Author: Erva RAKICI
Institution: Recep Tayyip Erdogan University

Author: Kadriye INAN BEKTAS
Institution: Karadeniz Technical University

Author: Sabriye ÇANAKÇI (Primary Author)
Institution: Karadeniz Technical University

Author: Ali Osman BELDUZ
Institution: Karadeniz Technical University

Dates

Publication Date : April 23, 2020

Bibtex @research article { jan554729, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {}, publisher = {Oktay YILDIZ}, year = {2020}, volume = {2}, pages = {30 - 45}, doi = {10.35206/jan.554729}, title = {Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities}, key = {cite}, author = {Özer, Ayşegül and Rakıcı, Erva and Inan Bektas, Kadriye and Çanakçı, Sabriye and Belduz, Ali Osman} }
APA Özer, A , Rakıcı, E , Inan Bektas, K , Çanakçı, S , Belduz, A . (2020). Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities . Journal of Apitherapy and Nature , 2 (2) , 30-45 . DOI: 10.35206/jan.554729
MLA Özer, A , Rakıcı, E , Inan Bektas, K , Çanakçı, S , Belduz, A . "Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities" . Journal of Apitherapy and Nature 2 (2020 ): 30-45 <https://dergipark.org.tr/en/pub/jan/issue/53848/554729>
Chicago Özer, A , Rakıcı, E , Inan Bektas, K , Çanakçı, S , Belduz, A . "Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities". Journal of Apitherapy and Nature 2 (2020 ): 30-45
RIS TY - JOUR T1 - Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities AU - Ayşegül Özer , Erva Rakıcı , Kadriye Inan Bektas , Sabriye Çanakçı , Ali Osman Belduz Y1 - 2020 PY - 2020 N1 - doi: 10.35206/jan.554729 DO - 10.35206/jan.554729 T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 30 EP - 45 VL - 2 IS - 2 SN - -2667-4734 M3 - doi: 10.35206/jan.554729 UR - https://doi.org/10.35206/jan.554729 Y2 - 2020 ER -
EndNote %0 Apiterapi ve Doğa Dergisi Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities %A Ayşegül Özer , Erva Rakıcı , Kadriye Inan Bektas , Sabriye Çanakçı , Ali Osman Belduz %T Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities %D 2020 %J Journal of Apitherapy and Nature %P -2667-4734 %V 2 %N 2 %R doi: 10.35206/jan.554729 %U 10.35206/jan.554729
ISNAD Özer, Ayşegül , Rakıcı, Erva , Inan Bektas, Kadriye , Çanakçı, Sabriye , Belduz, Ali Osman . "Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities". Journal of Apitherapy and Nature 2 / 2 (April 2020): 30-45 . https://doi.org/10.35206/jan.554729
AMA Özer A , Rakıcı E , Inan Bektas K , Çanakçı S , Belduz A . Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities. J.Apit.Nat.. 2020; 2(2): 30-45.
Vancouver Özer A , Rakıcı E , Inan Bektas K , Çanakçı S , Belduz A . Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities. Journal of Apitherapy and Nature. 2020; 2(2): 30-45.