First Report on Bioaccessibility, Antioxidant Activity and Total Phenolic Compounds From Stachys thirkei C.Koch Using A Simulated In Vitro Digestion System

Year 2022, Volume: 8 Issue: 2, 188 - 200, 23.06.2022

Emine Aydın Pınar Göç Rasgele Gorkem Dulger

https://doi.org/10.28979/jarnas.1014968

Cited By: 1

Abstract

It is of great importance to determine the antioxidant properties of plants, especially those used for food, pharmacology and medicinal purposes. Stachys thirkei C.Koch belonging to Lamiaceae family is used as a medicinal aromatic plant in Turkey. The present study was carried out to investigate the total phenolic contents, antioxidant activity and bioaccessibility of S. thirkei C.Koch. The total phenolic content (TPC) was evaluated by Folin-Ciocalteu colorimetric procedure and antioxidant activity determine four distinctive methods (ABTS•+, CUPRAC, DPPH• and FRAP). According to the results, the levels of hydrolysable phenolics (1538.99±4.57 mg of GAE/100g) approximately three and a half fold higher than extractable phenolics (422.96±4.70 mg of GAE/100g). The TPC of S. thirkei C.Koch was determined to be 1961.95±4.46 mg of GAE/100g. On the other hand, the bioaccessible fractions and phenolic bioaccessibility of S. thirkei C.Koch were found to be 1766.72±2.96 μmol Trolox/g and 90.05±1.50%, respectively. At the same time, the antioxidative bioaccessibility of S. thirkei was found to be higher in FRAP method (1164.29 μmol Trolox/g) and also the bioaccessibility (%) of S. thirkei C.Koch was found to be higher in CUPRAC method (93.41%). Present study is the first report to investigate the antioxidant effect and bioaccessibility of the extracts from S. thirkei C.Koch. The results of the present study can be strong scientific proof to use this plant as a beneficial source of antioxidant referments and the studies can be further extended to investigate whether they exhibit similar activities in in vivo systems.

Keywords

Medicinal Plant, Biological Activity, DPPH, ABTS, FRAP, CUPRAC

References

• Alan, S., Ozkan, Y., & Tuncer, O. (2010). Taxonomical, morphological and anatomical studies on Lallemantia Fisch. & Mey. J Fac Pharm Ankara, 39(1), 17-43. DOI: https://doi.org/10.1501/Eczfak_0000000551
• Alpay, M., Dulger, G., & Karabacak, E. (2017). Antioxidant, antimicrobial and antitumoral effects of Stachys annua (L.) L. subs. annua var. annua in comparative cancer profiles. Indian J Med Res Pharm, 4(12), 68-74. DOI: https://doi.org/10.5281/zenodo.1117666
• Amarowicz, R., Estrella, I., Hernández, T., Robredo, S., Troszyńska, A., Kosińska, A., & Pegg, R.B. (2010). Free radical scavenging capacity, antioxidant capacity, and phenolic composition of gren lentil (Lens culinaris). Food Chem, 121(3), 705-711. DOI: https://doi.org/10.1016/j.foodchem.2010.01.009
• Apak, R., Guclu, K., Ozyurek, M., & Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols, vitamin C and E using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem, 52, 7970-7981. DOI: https://doi.org/10.1021/jf048741x
• Apak, R., Guclu, K., Demirata, B., Ozyurek, M., Celik, E.S., Bektasoglu, B., Berker, K.I., & Ozyurt, D. (2007). Comparative evaluation of total antioxidant capacity assays applied to phenolic compounds and the CUPRAC Assay. Molecules, 12, 1496-1547. DOI: https://doi.org/10.3390/12071496
• Askun, T., Tekwu, E.M., Satil, F., Modanlioglu, S., & Aydeniz, H. (2013). Preliminary antimycobacterial study on selected Turkish plants (Lamiaceae) against Mycobacterium tuberculosis and search for some phenolic constituents. BMC Complem. Altern Med, 13, 365-375. DOI: https://doi.org/10.1186/1472-6882-13-365
• Aydemir, B., & Sari, E.K. (2009). Antioksidanlar ve Büyüme Faktörleri ile İlişkisi. Kocatepe Vet J, 2(2), 56-60. Retrieved from: https://dergipark.org.tr/tr/download/article-file/108794
• Bashi, S.D., Dowom, S.A., Bazzaz, B.S.F., Khanzadeh, F., Soheili, V., & Mohammadpour, A. (2016). Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. Iran J Basic Med Sci, 19, 529-541. Retrieved from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923474/pdf/IJBMS-19-529.pdf
• Benzie, I.F.F., & Strain, J.J. (2002). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP Assay. Anal Biochem, 239, 70-76. DOI: https://doi.org/10.1006/abio.1996.0292
• Beyzi, E. (2011). The effects of different phosphorus doses on yield and some morphological characters of fenugreek (Trigonella foenum-graecum L.) (Master’s thesis). Retrieved from: https://tez.yok.gov.tr/UlusalTezMerkezi
• Brand-Williams, W., Cavalier, M.E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Food Sci Technol, 28(1), 25-30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
• Bursal, E. (2009). Determination of antioxidant and antiradical activities of kiwifruit (Actinidia deliciosa), purification and characterisation of carbonic anhydrase from kiwifruit. (Master’s thesis). Retrieved from: https://tez.yok.gov.tr/UlusalTezMerkezi
• Carvalho, M.S.S., Cardoso, M.D.G., Resende, L.V., Gomes, M.D.S., Albuquerque, L.R.M., Gomes, A.C.S., Sales, T.A., Camargo, K.C., Nelson, D.L., Costa, G.M., Espósito, M.A., & Silva, L.F.L. (2015). Phytochemical screening, extraction of essential oils and antioxidant activity of five species of unconventional vegetables. Am J Plant Sci, 6, 2632-2639. DOI: https://doi.org/10.4236/ajps.2015.616265
• Chu, Y.H., Chang, C.L., & Hsu, H.F. (2000). Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agr, 80(5), 561-6. DOI: https://doi.org/10.1002/(SICI)1097-0010(200004)
• Daly, T., Jiwan, M.A., O’brien, N.M., & Aherne, S.A. (2010). Carotenoid content of commonly consumed herbs and assessment of their bioaccessibility using an in vitro digestion model. Plant Foods Hum Nutr, 65, 164-169. DOI: https://doi.org/10.1007/s11130-010-0167-3
• Ferhat, M., Erol, E., Beladjila, K.A., Cetintas, Y., Duru, M.E., Ozturk, M., Kabouche, A., & Kabouche, Z. (2016). Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatic. Pharm Biol, 55(1), 324-329. DOI: https://doi.org/10.1080/13880209.2016.1238488
• Fernandez-Garcia, E., Carvajal-Lerida, I., & Perez-Galvez, A. (2009). In vitro bioaccessibility assessment as a prediction tool of nutritional efficiency. Nutr Res, 29, 751-760. DOI: https://doi.org/10.1016/j.nutres.2009.09.016.
• Hajimehdipoor, H., Gohari, A.R., Ajani, Y., & Saeidnia, S. (2014). Comparative study of the total phenol content and antioxidant activity of some medicinal herbal extracts. Reser J Pharmacog, 1(3), 21-25.
• Halliwell, B. 1999. Establishing the Significance and Optimal Intake of Dietary Antioxidants: The Biomarker Concept. Nut Rev, 57(4), 104–113. DOI: https://doi.org/10.1111/j.1753-4887.1999.tb06933.x
• Háznagy-Radnai, E., Czigle, S.Z., Zupkó, I., Falkay, G.Y., & Máthé, I. (2006). Comparison of antioxidant activity in enzyme-independent system of six Stachys species. Fitoterapia, 77, 521-524. DOI: https://doi.org/10.1016/j.fitote.2006.06.007
• Gayoso, L., Roxo, M., Cavero, R.Y., Calvo, M.I., Ansorena, D., Astiasarán, I., & Wink, M. (2018). Bioaccessibility and biological activity of Melissa officinalis, Lavandula latifolia and Origanum vulgare extracts: Influence of an in vitro gastrointestinal digestion. J Func Foods, 44, 146-154. DOI: https://doi.org/10.1016/j.jff.2018.03.003
• Ghaffari, H., Ghassam, B.J., & Prakash, H.S. (2012). Evaluation of antioxidant and antiinflammatory activity of Stachys lavandulifolia. Int J Pharm Pharm Sci, 4(3), 691-696. Retrieved from: https://www.researchgate.net/publication/267037229_Evaluation_of_antioxidant_and_antiinflammatory_activity_of_Stachys_lavandulifolia
• Goren, A.C., Piozzi, F., Akcicek, E., Kilic, T., Carikci, S., Mozioglu, E. & Setzer, W.N. (2011). Essential oil composition of twenty-two Stachys species (mountain tea) and their biological activities. Phytochem Let, 4, 448-453. DOI: https://doi.org/10.1016/j.phytol.2011.04.013
• Gulcin, I. (2006). Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicol, 217, 213-220. DOI: https://doi.org/10.1016/j.tox.2005.09.011
• Koleva, I.I., van Beek, T.A., Linssen, J.P.H., de Groot, A., & Evstatieva, L.N. (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal, 13(1), 8-17. DOI: https://doi.org/10.1002/pca.611
• Konak, M., Ates, M., & Sahan, Y. (2017). Evaluation of antioxidant properties of Gundelia tournefortii: a wild edible plant. Journal of Agricultural Faculty of Uludag University, 31(2), 101-108. Retrieved from: https://www.cabdirect.org/cabdirect/FullTextPDF/2018/20183140202.pdf
• Labanca, R.A., Svelander, C., & Alminger, M. (2019). Effect of particle size of chia seeds on bioaccessibility of phenolic compounds during in vitro digestion. Cogent Food and Agricul, 5(1), 1-13. DOI: https://doi.org/10.1080/23311932.2019.1694775
• Lakhal, H., Boudiar, T., Kabouche, A., Laggoune, S., Kabouche, Z., & Topcu, G. (2011). Antioxidant activity and flavonoids of Stachys ocymastrum. Chem Nat Comp, 46(6), 964-965. DOI: https://doi.org/10.1007/s10600-011-9797-4
• Leblebici, S. (2011). An investigation of the anatomical and ecological on endemic species of the Stachys sp. spreading in Kütahya and Eskişehir (Master’s thesis). Retrieved from: https://tez.yok.gov.tr/UlusalTezMerkezi
• Leporini, L., Menghini, L., Foddai, M., Petretto, G.L., Chessa, M., Tirillini, B., & Pintore, G. (2015). Antioxidant and antiproliferative activity of Stachys glutinosa L. ethanol extract. Nat Prod Res, 29(10), 899-907. DOI: https://doi.org/10.1080/14786419.2014.955490
• Mantle, D., Eddeb, F., & Pickering, A.T. (2000). Comparison of relative antioxidant activities of British medicinal plant species in vitro. J Ethnopharmaco, 72(1-2), 47-51. DOI: https://doi.org/10.1016/S0378-8741(00)00199-9
• Oke, J.M., & Hamburger, M.O. (2002). Screening of some Nigerian medicinal plants for antioxidant activity using 2,2,di-phenyl-picryl-hydrazyl radical. Afr J Biomed Res, 5(1-2), 77-79. Retrieved from: file:///C:/Users/User/Downloads/53985-Article%20Text-86718-1-10-20100421.pdf
• Ozkan, G., Gokturk, R.S., Unal, O., & Celik, S. (2006). Determination of the volatile constituents and total phenolic contents of some endemic Stachys taxa from Turkey. Chem Nat Comp, 42(2), 172-174. DOI: https://doi.org/:10.1007/s10600-006-0070-1
• Pellegrini, M., Lucas-Gonzalez, R., Sayas-Barberá, E., Fernández-López, J., Pérez-Álvarez, J.A., & Viuda-Martos, M. (2018). Bioaccessibility of phenolic compounds and antioxidant capacity of chia (Salvia hispanica L.) seeds. Plant Foods Hum Nutr, 73, 47-53. DOI: htpps://doi.org/10.1007/s11130-017-0649-7
• Prior, R.L. (2003). Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr, 78(3), 570-578. DOI: https://doi.org/10.1093/ajcn/78.3.570S
• Rahimi Khoigani, S., Rajaei, A., & Goli, S.A.H. (2017). Evaluation of antioxidant activity, total phenolics, total flavonoids and LC–MS/MS characterization of phenolic constituents in Stachys lavandulifolia. Nat Prod Res, 31(3), 355-358. DOI: https://doi.org/10.1080/14786419.2016.1233410
• Rasgele, P.G., & Dulger, G. (2021). Chemical compositions and antimutagenic effects of etanolic extracts of Stachys thirkei and Stachys annua subsp. annua using the ames assay. Pharmaceut Chem J, 54(12), 1255-1262. DOI: https://doi.org/10.1007/s11094-021-02351-x
• Rebellato, A.P., Pacheco, B.C., Prado, J.P., & Pallone, J.A.L. (2015). Iron in fortified biscuits: a simple method for its quantification, bioaccessibility study and physicochemical quality. Food Res Int, 77, 385-391. DOI: https://doi.org/10.1016/j.foodres.2015.09.028
• Schuler, P. (1990). Natural antioxidants exploited commercially. In: Hudson BJF. (ed.) Food Antioxidants. Elsevier: London. p. 99-170. Retrieved from: https://www.researchgate.net/publication/279360267_Natural_Antioxidants_Exploited_Commercially
• Silinsin, M. (2016). Determination of in vitro antioxidant activities of water and ethanol extracts of Inula graveolens (L.) Desf. (Master’s thesis). Retrieved from: https://tez.yok.gov.tr/UlusalTezMerkezi
• ˇSliumpait ̇e. I., Venskutonisa, P.R., Murkovic, M., & Ragaˇzinskien ̇e, O. (2013). Antioxidant properties and phenolic composition of wood betony (Betonica officinalis L., syn. Stachys officinalis L.). Industr Crops and Prod, 50, 715-722. DOI: https://doi.org/10.1016/j.indcrop.2013.08.024
• Sytar, O., Hemmerich, I., Zivcak, M., Rauh, C., & Brestic, M. (2018). Comparative analysis of bioactive phenolic compounds composition from 26 medicinal plants. Saudi J Biol Sci, 25, 631-641. DOI: https://doi.org/10.1016/j.sjbs.2016.01.036
• Tepe, B., Degerli, S., Arslan, S., Malatyali, E., & Sarikurkcu, C. (2011). Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia, 82, 237-246. DOI: https://doi.org/10.1016/j.fitote.2010.10.006
• Tunali Erkan, D., & Dulger, B. (2016). The studies on antimicrobial activity of the plant Stachys thirkei. Düzce University J of Sci Technol, 4, 886-893. Retrieved from: https://dergipark.org.tr/tr/download/article-file/224873
• Unsal, C., Vural, H., Sariyar, G., Ozbek, B., & Otuk, G. (2010). Traditional medicine in Bilecik province (Turkey) and antimicrobial activities of selected species. Turk J Pharm Sci, 7(2), 139-150. Retrieved from: http://www.turkjps.org/archives/archive-detail/article-preview/tradtonal-medcne-n-bleck-provnce-turkey-and-antmcr/12485
• Usal, M., & Sahan, Y. (2020). In vitro evaluation of the bioaccessibility of antioxidative properties in commercially baby foods. J Food Sci Technol, 57(9), 3493-3501. DOI: https://doi.org/10.1007/s13197-020-04384-8
• Valadez-Carmona, L., Cortez-García, R.M., Plazola-Jacinto, C.P., Necoechea Mondragón, H., & Ortiz-Moreno, A. (2016). Effect of microwave drying and oven drying on the water activity, color, phenolic compounds content and antioxidant activity of coconut husk (Cocos nucifera L.). J Food Sci Technol, 53(9), 3495-3501. DOI: https://doi.org/10.1007/s13197-016-2324-7
• Venditti, A., Bianco, A., Quassinti, L., Bramucci, M., Lupidi, G., Damiano, S., Papa, F., Vittori, S., Bini, L.M., Giuliani, C., Lucarini, D., & Maggi, F. (2015). Phytochemical analysis, biological activity, and secretory structures of Stachys annua (L.) L. subsp. annua (Lamiaceae) from central Italy. Chem & Biodiver, 12, 1172-1183. DOI: https://doi.org/10.1002/cbdv.201400275
• Vitali, D., Vedrina Dragojevic, I., & Sˇebecic, B. (2009). Effects of incorporation of integral raw materials and dietary fiber on the selected nutritional and functional properties of biscuits. Food Chem, 114, 1462-1469. DOI: https://doi.org/10.1016/j.foodchem.2008.11.032
• Xu, J.G., Tian, C.R., Hu, Q.P., Luo, J.Y., Wang, X.D., & Tian, X.D. (2009). Dynamic changes in phenolic compounds and antioxidant activity in oats (Avena nuda L.) during steeping and germination. J Agric Food Chem, 57,10392-10398. DOI: https://doi.org/10.1021/jf902778j
• Yang, M.R., No, G.R., Kang, S.N., Kim, T.V., Kim, S.M., & Kim, I.S. (2016). Antioxidant and antimicrobial activities of various Stachys sieboldii Miq. extracts for application in meat product. Indian J Appl Res, 6(9), 70-75. Retrieved from: https://www.worldwidejournals.com/indian-journal-of-applied-research-(IJAR)/fileview/September_2016_1492162457__201.pdf
• Yu, B.P. (1994). Cellular defenses against damage from reactive oxygen species. Physiol Rev, 74(1), 139-62. DOI: https://doi.org/10.1152/physrev.1994.74.1.139