<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.4 20241031//EN"
        "https://jats.nlm.nih.gov/publishing/1.4/JATS-journalpublishing1-4.dtd">
<article  article-type="reviewer-report"        dtd-version="1.4">
            <front>

                <journal-meta>
                                                                <journal-id>jase</journal-id>
            <journal-title-group>
                                                                                    <journal-title>Journal of Animal Science and Economics</journal-title>
            </journal-title-group>
                                        <issn pub-type="epub">2980-1486</issn>
                                                                                            <publisher>
                    <publisher-name>Ataturk University</publisher-name>
                </publisher>
                    </journal-meta>
                <article-meta>
                                        <article-id pub-id-type="doi">10.5281/zenodo.10731544</article-id>
                                                                <article-categories>
                                            <subj-group  xml:lang="en">
                                                            <subject>Veterinary Sciences (Other)</subject>
                                                    </subj-group>
                                            <subj-group  xml:lang="tr">
                                                            <subject>Veteriner Bilimleri (Diğer)</subject>
                                                    </subj-group>
                                    </article-categories>
                                                                                                                                                        <title-group>
                                                                                                                        <trans-title-group xml:lang="tr">
                                    <trans-title>İneklerde Gebelik Tanı Yöntemleri</trans-title>
                                </trans-title-group>
                                                                                                                                                                                                <article-title>Pregnancy Diagnosis Methods in Cows</article-title>
                                                                                                    </title-group>
            
                                                    <contrib-group content-type="authors">
                                                                        <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-7912-7703</contrib-id>
                                                                <name>
                                    <surname>Çiplak</surname>
                                    <given-names>Alper Yasin</given-names>
                                </name>
                                                                    <aff>ATATÜRK ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ</aff>
                                                            </contrib>
                                                                                </contrib-group>
                        
                                        <pub-date pub-type="pub" iso-8601-date="20240228">
                    <day>02</day>
                    <month>28</month>
                    <year>2024</year>
                </pub-date>
                                        <volume>3</volume>
                                        <issue>1</issue>
                                        <fpage>23</fpage>
                                        <lpage>29</lpage>
                        
                        <history>
                                    <date date-type="received" iso-8601-date="20240108">
                        <day>01</day>
                        <month>08</month>
                        <year>2024</year>
                    </date>
                                                    <date date-type="accepted" iso-8601-date="20240223">
                        <day>02</day>
                        <month>23</month>
                        <year>2024</year>
                    </date>
                            </history>
                                        <permissions>
                    <copyright-statement>Copyright © 2023, Journal of Animal Science and Economics</copyright-statement>
                    <copyright-year>2023</copyright-year>
                    <copyright-holder>Journal of Animal Science and Economics</copyright-holder>
                </permissions>
            
                                                                                                <trans-abstract xml:lang="tr">
                            <p>Büyükbaş hayvan çiftliklerinde süt veriminin ve doğurganlığın optimize edilmesi sürdürülebilirlik ve ekonomi açısından büyük önem taşımaktadır. Bu parametreleri optimize etmek için her ineğin 12-14 ayda bir buzağılaması gerekmektedir. Bu amaçla ineklerin postpartum en erken dönemde gebe kalmaları ve derhal gebelik muayenelerinden geçmeleri gerekmektedir. Sığır çiftliklerinde gebeliğin erken teşhisi üreme yönetimi açısından önemlidir. Gebelik tanı yöntemleri direkt ve indirekt olmak üzere iki gruba ayrılmaktadır. Direkt tanı yöntemleri gebeliğin durumunu objektif olarak belirler. İndirekt tanı yöntemleri ise gebelik sırasında anne dolaşımında oluşan biyobelirteçler aracılığıyla gebelik hakkında bilgi sağlamaktadır. İdeal bir gebelik testi erken gebelikte doğru sonuçlar verebilmeli (duyarlılığı ve özgüllüğü yüksek), maliyeti düşük ve uygulaması kolay olmalıdır. Günümüzde gebelik tanısı amacıyla çoğunlukla direkt tanı yöntemleri kullanılmakla birlikte, gelişen teknoloji ve bilimsel çalışmalardaki ilerleme sonucunda indirekt tanı yöntemlerinin kullanım sıklığının artacağı ve yeni yöntemlerin geliştirileceği öngörülmektedir. Bu derlemenin amacı, ineklerde gebelik teşhisinde kullanılabilecek yöntemler, bu yöntemlerin nasıl uygulandığı, birbirlerine göre avantaj ve dezavantajları hakkında okuyucuları bilgilendirmektir.</p></trans-abstract>
                                                                                                                                    <abstract><p>In cattle farms, optimising milk yield and fertility is crucial in terms of sustainability and economics. In order to optimise these parameters, each cow should have a calf every 12-14 months. For this purpose, cows should be pregnant during the earliest postpartum period and undergo pregnancy examinations promptly. Early diagnosis of pregnancy on cattle farms is essential for reproductive management. Methods of pregnancy diagnosis are divided into two groups, direct and indirect. Direct diagnostic methods objectively determine the state of pregnancy. Indirect diagnostic methods provide information about pregnancy through biomarkers formed in the maternal circulation during pregnancy. An ideal pregnancy test should be able to give accurate results in early pregnancy (high sensitivity and specificity) and should be low cost and easy to apply. Although direct diagnostic methods are the most commonly used today, it is predicted that the frequency of use of indirect diagnostic methods will increase and new methods will be developed as a result of developing technology and progress in scientific studies. The present review aims to inform the readers about the methods that can be used in the diagnosis of pregnancy in cows, how these methods are performed, their advantages and disadvantages compared to each other.</p></abstract>
                                                            
            
                                                                                        <kwd-group>
                                                    <kwd>Cow</kwd>
                                                    <kwd>  Direct diagnosis</kwd>
                                                    <kwd>  Indirect diagnosis</kwd>
                                                    <kwd>  Pregnancy diagnosis</kwd>
                                            </kwd-group>
                            
                                                <kwd-group xml:lang="tr">
                                                    <kwd>İnek</kwd>
                                                    <kwd>  Direkt tanı</kwd>
                                                    <kwd>  Gebelik tanısı</kwd>
                                                    <kwd>  İndirekt tanı</kwd>
                                            </kwd-group>
                                                                                                                                        </article-meta>
    </front>
    <back>
                            <ref-list>
                                    <ref id="ref1">
                        <label>1</label>
                        <mixed-citation publication-type="journal">Akköse, M., &amp; Çiğdem, Ç. (2019). Sütçü Sığırlarda Erken Gebelik Tanısı. Hayvansal Üretim, 60(2), 171-179.</mixed-citation>
                    </ref>
                                    <ref id="ref2">
                        <label>2</label>
                        <mixed-citation publication-type="journal">Alaçam, E. (2015). Gebelik Tanısı. Evcil Hayvanlarda Doğum ve İnfertilite. Eighth Edition (pp. 115-126). Medisan, Ankara, TR.</mixed-citation>
                    </ref>
                                    <ref id="ref3">
                        <label>3</label>
                        <mixed-citation publication-type="journal">Balhara, A. K., Gupta, M., Singh, S., Mohanty, A. K., &amp; Singh, I. (2013). Early pregnancy diagnosis in bovines: current status and future directions. The Scientific World Journal.</mixed-citation>
                    </ref>
                                    <ref id="ref4">
                        <label>4</label>
                        <mixed-citation publication-type="journal">Baştan, A., Özenç, E., Macun, H. C., Acar, D. B., &amp; Güngör, Ö. (2007). Use of early conception factor test for determining pregnancy and embryonic mortality status of dairy cows. Medycyna Weterynaryjna, 63(6), 670-673.</mixed-citation>
                    </ref>
                                    <ref id="ref5">
                        <label>5</label>
                        <mixed-citation publication-type="journal">Boyd, J. S., Omran, S. N., &amp; Ayliffe, T. R. (1990). Evaluation of real time B-mode ultrasound scanning for detecting early pregnancy in cows. Veterinary Record, 127(14), 350-352.</mixed-citation>
                    </ref>
                                    <ref id="ref6">
                        <label>6</label>
                        <mixed-citation publication-type="journal">Cavanagh, A. C. (1996). Identification of early pregnancy factor as chaperonin 10: implications for understanding its role. Reviews of Reproduction, 1(1), 28-32.</mixed-citation>
                    </ref>
                                    <ref id="ref7">
                        <label>7</label>
                        <mixed-citation publication-type="journal">Christiansen, D. (2014). Pregnancy Diagnosis: Rectal Palpation. Bovine Reproduction (pp. 314-319). Wiley-Blackwell, USA.‎</mixed-citation>
                    </ref>
                                    <ref id="ref8">
                        <label>8</label>
                        <mixed-citation publication-type="journal">Cruz, Y. P., Selwood, L., Morton, H., &amp; Cavanagh, A. C. (2001). Significance of serum early pregnancy factor concentrations during pregnancy and embryonic development in Sminthopsis macroura (Spencer) (Marsupialia: Dasyuridae). Reproduction, 121(6), 933-939.</mixed-citation>
                    </ref>
                                    <ref id="ref9">
                        <label>9</label>
                        <mixed-citation publication-type="journal">de Melo, G. D., Mello, B. P., Ferreira, C. A., Godoy Filho, C. A. S., Rocha, C. C., Silva, A. G., Reese, S. T., Madureira, E. H., Pohler, K. G., &amp; Pugliesi, G. (2020). Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle. Theriogenology, 152, 94-105.</mixed-citation>
                    </ref>
                                    <ref id="ref10">
                        <label>10</label>
                        <mixed-citation publication-type="journal">Diskin, M. G., Murphy, J. J., &amp; Sreenan, J. M. (2006). Embryo survival in dairy cows managed under pastoral conditions. Animal Reproduction Science, 96(3-4), 297-311.</mixed-citation>
                    </ref>
                                    <ref id="ref11">
                        <label>11</label>
                        <mixed-citation publication-type="journal">Ergene, O. (2008). İneklerde Progesteron Hormonunun Klinik Endikasyonları. Veteriner Hekimler Derneği Dergisi, 79(4), 29-34.</mixed-citation>
                    </ref>
                                    <ref id="ref12">
                        <label>12</label>
                        <mixed-citation publication-type="journal">Fetrow, J., Stewart, S., Eicker, S., &amp; Rapnicki, P. (2007). Reproductive Health Programs for Dairy Herds: Analysis of Records for Assessment of Reproductive Performance. Youngquist, R. S., Threlfall, W. R. eds. Second Edition. In: Current Therapy in Large Animal Theriogenology (pp. 473-489).  Saunders, USA.</mixed-citation>
                    </ref>
                                    <ref id="ref13">
                        <label>13</label>
                        <mixed-citation publication-type="journal">Fiandanese, N., Viglino, A., Strozzi, F., Stella, A., Williams, J. L., Lonergan, P., Forde, N., &amp; Iamartino, D. (2016). Circulating microRNAs as potential biomarkers of early pregnancy in high producing dairy cows. Reproduction, Fertility and Development, 28(2), 165.</mixed-citation>
                    </ref>
                                    <ref id="ref14">
                        <label>14</label>
                        <mixed-citation publication-type="journal">Fricke, P. M., Ricci, A., Giordano, J. O., &amp; Carvalho, P. D. (2016). Methods for and implementation of pregnancy diagnosis in dairy cows. Veterinary Clinics of North America: Food Animal Practice, 32(1), 165-180.</mixed-citation>
                    </ref>
                                    <ref id="ref15">
                        <label>15</label>
                        <mixed-citation publication-type="journal">Gábor, G., Tóth, F., Ozsvári, L., Abonyi‐Tóth, Z., &amp; Sasser, R. G. (2007). Early detection of pregnancy and embryonic loss in dairy cattle by ELISA tests. Reproduction in Domestic Animals, 42(6), 633-636.</mixed-citation>
                    </ref>
                                    <ref id="ref16">
                        <label>16</label>
                        <mixed-citation publication-type="journal">Ganaie, B. A., Khan, M. Z., Islam, R., Makhdoomi, D. M., Qureshi, S., &amp; Wani, G. M. (2009). Evaluation of different techniques for pregnancy diagnosis in sheep. Small Ruminant Research, 85(2-3), 135-141.</mixed-citation>
                    </ref>
                                    <ref id="ref17">
                        <label>17</label>
                        <mixed-citation publication-type="journal">Garbayo, J. M., Serrano, B., &amp; Lopez-Gatius, F. (2008). Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants. Animal Reproduction Science, 103(1-2), 120-134.</mixed-citation>
                    </ref>
                                    <ref id="ref18">
                        <label>18</label>
                        <mixed-citation publication-type="journal">Gifford, C. A., Racicot, K., Clark, D. S., Austin, K. J., Hansen, T. R., Lucy, M. C., Davies, C. J., &amp; Ott, T. L. (2007). Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Journal of Dairy Science, 90(1), 274-280.</mixed-citation>
                    </ref>
                                    <ref id="ref19">
                        <label>19</label>
                        <mixed-citation publication-type="journal">Gilad, S., Meiri, E., Yogev, Y., Benjamin, S., Lebanony, D., Yerushalmi, N., Benjamin, H., Kushnir, M., Cholakh, H., Melamed, N., Bentwich, Z., Hod, M., Goren, Y., &amp; Chajut, A. (2008). Serum microRNAs are promising novel biomarkers. PLoS One, 3(9), e3148.</mixed-citation>
                    </ref>
                                    <ref id="ref20">
                        <label>20</label>
                        <mixed-citation publication-type="journal">Ginther, O. J. (2007). Ultrasonic Imaging and Animal Reproduction: Color-Doppler Ultrasonography. Equiservices Publishing, Cross Plaons, WI, USA.</mixed-citation>
                    </ref>
                                    <ref id="ref21">
                        <label>21</label>
                        <mixed-citation publication-type="journal">Green, J. A., Xie, S., Quan, X., Bao, B., Gan, X., Mathialagan, N., Beckers, J. F., &amp; Roberts, R. M. (2000). Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biology of Reproduction, 62(6), 1624-31.</mixed-citation>
                    </ref>
                                    <ref id="ref22">
                        <label>22</label>
                        <mixed-citation publication-type="journal">Green, J. C., Okamura, C. S., Poock, S. E., &amp; Lucy, M. C. (2010). Measurement of interferon-tau (IFN-τ) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18–20 d after insemination in dairy cattle. Animal Reproduction Science, 121(1-2), 24-33.</mixed-citation>
                    </ref>
                                    <ref id="ref23">
                        <label>23</label>
                        <mixed-citation publication-type="journal">Grewal, A. S., Wallace, A. L. C., Pan, Y. S., Rigby, N. W., Donnelly, J. B., Eagleson, G. K., &amp; Nancarrow, C. D. (1985). Evaluation of a rosette inhibition test for pregnancy diagnosis in pigs. Journal of Reproductive Immunology, 7(2), 129-138.</mixed-citation>
                    </ref>
                                    <ref id="ref24">
                        <label>24</label>
                        <mixed-citation publication-type="journal">Hansen, T. R., Sinedino, L. D., &amp; Spencer, T. E. (2017). Paracrine and endocrine actions of interferon tau (IFNT). Reproduction, 154(5), 45-59.</mixed-citation>
                    </ref>
                                    <ref id="ref25">
                        <label>25</label>
                        <mixed-citation publication-type="journal">Hanzen, C., &amp; Delsaux, B. (1987). Use of transrectal B-mode ultrasound imaging in bovine pregnancy diagnosis. Veterinary Record, 121(9), 200-202.</mixed-citation>
                    </ref>
                                    <ref id="ref26">
                        <label>26</label>
                        <mixed-citation publication-type="journal">Ioannidis, J., &amp; Donadeu, F. X. (2016). Circulating miRNA signatures of early pregnancy in cattle. BMC Genomics, 17(1), 1-12.</mixed-citation>
                    </ref>
                                    <ref id="ref27">
                        <label>27</label>
                        <mixed-citation publication-type="journal">Isobe, N., Nakao, T., Uehara, O., Yamashiro, H., &amp; Kubota, H. (2003). Plasma concentration of estrone sulfate during pregnancy in different breeds of Japanese beef cattle. Journal of Reproduction and Development, 49(5), 369-374.</mixed-citation>
                    </ref>
                                    <ref id="ref28">
                        <label>28</label>
                        <mixed-citation publication-type="journal">Jaskowski, J. M., Kaczmarowski, M., Kulus, J., Jaskowski, B. M., Herudzinska, M., &amp; Gehrke, M. (2019). Rectal palpation for pregnancy in cows: A relic or an alternative to modern diagnostic methods. Medycyna Weterynaryjna, 75(5), 259-264.</mixed-citation>
                    </ref>
                                    <ref id="ref29">
                        <label>29</label>
                        <mixed-citation publication-type="journal">Kumar, P. R., Singh, S. K., Kharche, S. D., Govindaraju, C. S., Behera, B. K., Shukla, S. N., Kumar, H., &amp; Agarwal, S. K. (2014). Anestrus in cattle and buffalo: Indian perspective. Advances in Animal and Veterinary Sciences, 2(3), 124-138.</mixed-citation>
                    </ref>
                                    <ref id="ref30">
                        <label>30</label>
                        <mixed-citation publication-type="journal">Kurian, N. K., &amp; Modi, D. (2019). Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy. Journal of Assisted Reproduction and Genetics, 36, 189-198.</mixed-citation>
                    </ref>
                                    <ref id="ref31">
                        <label>31</label>
                        <mixed-citation publication-type="journal">Lash, G. E., Legge, M., &amp; Fisher, M. (1997). Synthesis of early pregnancy factor using red deer (Cervus elaphus) as a delayed implantation model. Journal of Assisted Reproduction and Genetics, 14, 39-43.</mixed-citation>
                    </ref>
                                    <ref id="ref32">
                        <label>32</label>
                        <mixed-citation publication-type="journal">Lobago, F., Bekana, M., Gustafsson, H., Beckers, J. F., Yohannes, G., Aster, Y., &amp; Kindahl, H. (2009). Serum profiles of pregnancy‐associated glycoprotein, oestrone sulphate and progesterone during gestation and some factors influencing the profiles in Ethiopian Borana and crossbred cattle. Reproduction in Domestic Animals, 44(4), 685-692.</mixed-citation>
                    </ref>
                                    <ref id="ref33">
                        <label>33</label>
                        <mixed-citation publication-type="journal">Mimoune, N., Azzouz, M. Y., Khelef, D., &amp; Kaidi, R. (2021). Ovarian cysts in cattle: a review. Veterinarska Stanica, 52(5), 587-603.</mixed-citation>
                    </ref>
                                    <ref id="ref34">
                        <label>34</label>
                        <mixed-citation publication-type="journal">Morton, H., Clunie, G. J., &amp; Shaw, F. D. (1979). A test for early pregnancy in sheep. Research in Veterinary Science, 26(2), 261–262.</mixed-citation>
                    </ref>
                                    <ref id="ref35">
                        <label>35</label>
                        <mixed-citation publication-type="journal">Nancarrow, C. D., Wallace, A. L. C., &amp; Grewal, A. S. (1981). The early pregnancy factor of sheep and cattle. Journal of Reproduction and Fertility Supplement, 30, 191-199.</mixed-citation>
                    </ref>
                                    <ref id="ref36">
                        <label>36</label>
                        <mixed-citation publication-type="journal">Ohnuma, K., Ito, K., Miyake, Y. I., Takahashi, J., &amp; Yasuda, Y. (1996). Detection of early pregnancy factor (EPF) in mare sera. Journal of Reproduction and Development, 42(1), 23-28.</mixed-citation>
                    </ref>
                                    <ref id="ref37">
                        <label>37</label>
                        <mixed-citation publication-type="journal">Otavă, G., Cernescu, H., Mircu, C., &amp; Igna, V. (2007). Pregnancy diagnosis in cow using progesterone measurements. Lucrări Stiinłifice Medicină Veterinară, 95-98.</mixed-citation>
                    </ref>
                                    <ref id="ref38">
                        <label>38</label>
                        <mixed-citation publication-type="journal">Palomares, R. A., Walz, H. G., &amp; Brock, K. V. (2013). Expression of type I interferon-induced antiviral state and pro-apoptosis markers during experimental infection with low or high virulence bovine viral diarrhea virus in beef calves. Virus Research, 173(2), 260-269.</mixed-citation>
                    </ref>
                                    <ref id="ref39">
                        <label>39</label>
                        <mixed-citation publication-type="journal">Pohler, K. G., Franco, G. A., Reese, S. T., Dantas, F. G., Ellis, M. D., &amp; Payton, R. R. (2016). Past, Present and Future of Pregnancy Detection Methods. Applied Reproductive Strategies in Beef Cattle (pp. 7-8). Des Moines, Iowa.</mixed-citation>
                    </ref>
                                    <ref id="ref40">
                        <label>40</label>
                        <mixed-citation publication-type="journal">Pohler, K. G., Green, J. A., Geary, T. W., Peres, R. F. G., Pereira, M. H. C., Vasconcelos, J. L. M., &amp; Smith, M. F. (2015). Predicting embryo presence and viability. Regulation of Implantation and Establishment of Pregnancy in Mammals: Tribute to 45 Year Anniversary of Roger V. Short&#039;s Maternal Recognition of Pregnancy, (pp. 253-270).</mixed-citation>
                    </ref>
                                    <ref id="ref41">
                        <label>41</label>
                        <mixed-citation publication-type="journal">Romano, J. E., Thompson, J. A., Forrest, D. W., Westhusin, M. E., Tomaszweski, M. A., &amp; Kraemer, D. C. (2006). Early pregnancy diagnosis by transrectal ultrasonography in dairy cattle. Theriogenology, 66(4), 1034-1041.</mixed-citation>
                    </ref>
                                    <ref id="ref42">
                        <label>42</label>
                        <mixed-citation publication-type="journal">Romano, J. E., Thompson, J. A., Kraemer, D. C., Westhusin, M. E., Forrest, D. W., &amp; Tomaszweski, M. A. (2007). Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle. Theriogenology, 67(3), 486-493.</mixed-citation>
                    </ref>
                                    <ref id="ref43">
                        <label>43</label>
                        <mixed-citation publication-type="journal">Serrano, B., López‐Gatius, F., Santolaria, P., Almería, S., García‐Ispierto, I., Bech-Sabat, G., Sulon, J., De Sousa, N. M., Beckers, J. F., &amp; Yâniz, J. L. (2009). Factors affecting plasma pregnancy‐associated glycoprotein 1 concentrations throughout gestation in high‐producing dairy cows. Reproduction in Domestic Animals, 44(4), 600-605.</mixed-citation>
                    </ref>
                                    <ref id="ref44">
                        <label>44</label>
                        <mixed-citation publication-type="journal">Shah, K. D., Nakao, T., &amp; Kubota, H. (2006). Plasma estrone sulphate (E1S) and estradiol-17β (E2β) profiles during pregnancy and their relationship with the relaxation of sacrosciatic ligament, and prediction of calving time in Holstein–Friesian cattle. Animal Reproduction Science, 95(1-2), 38-53.</mixed-citation>
                    </ref>
                                    <ref id="ref45">
                        <label>45</label>
                        <mixed-citation publication-type="journal">Sheldon, M., &amp; Noakes, D. (2002). Pregnancy diagnosis in cattle. In Practice, 24(6), 310-317.</mixed-citation>
                    </ref>
                                    <ref id="ref46">
                        <label>46</label>
                        <mixed-citation publication-type="journal">Siqueira, L. G., Arashiro, E. K., Ghetti, A. M., Souza, E. D., Feres, L. F., Pfeifer, L. F., Fonseca, J. F., &amp; Viana, J. H. (2019). Vascular and morphological features of the corpus luteum 12 to 20 days after timed artificial insemination in dairy cattle. Journal of Dairy Science, 102(6), 5612-22.</mixed-citation>
                    </ref>
                                    <ref id="ref47">
                        <label>47</label>
                        <mixed-citation publication-type="journal">Smart, Y. C., Roberts, T. K., Fraser, I. S., Cripps, A. W., &amp; Clancy, R. L. (1982). Validation of the rosette inhibition test for the detection of early pregnancy in women. Fertility and Sterility, 37(6), 779-785.</mixed-citation>
                    </ref>
                                    <ref id="ref48">
                        <label>48</label>
                        <mixed-citation publication-type="journal">Telugu, B. P. V., Walker, A. M., &amp; Green, J. A. (2009). Characterization of the bovine pregnancy-associated glycoprotein gene family–analysis of gene sequences, regulatory regions within the promoter and expression of selected genes. BMC Genomics, 10(1), 1-17.</mixed-citation>
                    </ref>
                                    <ref id="ref49">
                        <label>49</label>
                        <mixed-citation publication-type="journal">Utt, M. D., Johnson Iii, G. L., &amp; Beal, W. E. (2009). The evaluation of corpus luteum blood flow using color-flow Doppler ultrasound for early pregnancy diagnosis in bovine embryo recipients. Theriogenology, 71(4), 707-715.</mixed-citation>
                    </ref>
                                    <ref id="ref50">
                        <label>50</label>
                        <mixed-citation publication-type="journal">Vural, R., Güzeloğlu, A., &amp; Küplülü, Ş. (2012). Gebelik ve Fizyolojisi. Semacan, A., Kaymaz, M., Fındık, M., Rişvanlı, A., &amp; Köker, A. eds. First Edition. In: Çiftlik Hayvanlarında Doğum ve Jinekoloji (pp. 125-155). Medipres, Malatya, TR.</mixed-citation>
                    </ref>
                                    <ref id="ref51">
                        <label>51</label>
                        <mixed-citation publication-type="journal">Youngquist, R. S. (2006). Pregnancy diagnosis using palpation, ultrasound, and blood testing. Proceedings, Applied Reproductive Strategies in Beef Cattle (pp. 329-338). St. Joseph, Missouri.</mixed-citation>
                    </ref>
                            </ref-list>
                    </back>
    </article>
