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Year 2020, Volume: 4 Issue: 3, 230 - 236, 01.09.2020
https://doi.org/10.30621/jbachs.2020.1057

Abstract

References

  • 1. Laville M, Juillard L. Contrast-induced acute kidney injury: how should at-risk patients be identified and managed? J Nephrol 2010;23:387– 398. https://pubmed.ncbi.nlm.nih.gov/20349411/
  • 2. Jeong BY, Lee HY, Park CG, et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 2018;13:e0191034. [CrossRef]
  • 3. Billings FTt, Chen SW, Kim M, et al. alpha2-Adrenergic agonists protect against radiocontrast-induced nephropathy in mice. Am J Physiol Renal Physiol 2008;295:F741–F748. [CrossRef]
  • 4. Wong PCY, Li Z, Guo J, Zhang A. Pathophysiology of contrast-induced nephropathy. Int J Cardiol 2012;158:186–192. [CrossRef]
  • 5. Bedard K, Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 2007;87:245– 313. [CrossRef]
  • 6. Frost JA, Xu S, Hutchison MR, Marcus S, Cobb MH. Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members. Mol Cell Biol 1996;16:3707–3713. [CrossRef]
  • 7. Lee HT, Jan M, Bae SC, et al. A1 adenosine receptor knockout mice are protected against acute radiocontrast nephropathy in vivo. Am J Physiol Renal Physiol 2006;290:F1367–F1375. [CrossRef]
  • 8. Gumustekin M, Micili SC, Arici MA, Karaman M, Guneli ME, Tekmen I. The Effect of Insulin Treatment on Rac1 Expression in Diabetic Kidney. Renal Failure 2013;35:396–402. [CrossRef]
  • 9. Desire L, Bourdin J, Loiseau N, et al. RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitro and in vivo. J Biol Chem 2005;280:37516– 37525. [CrossRef]
  • 10. Evcim AS, Micili SC, Karaman M, et al. The Role of Rac1 on Carbacholinduced Contractile Activity in Detrusor Smooth Muscle from Streptozotocin-induced Diabetic Rats. Basic Clin Pharmacol Toxicol 2015;116:476–84. [CrossRef]
  • 11. Yenicerioglu Y, Yilmaz O, Sarioglu S, et al. Effects of Nacetylcysteine on radiocontrast nephropathy in rats. Scand J Urol Nephrol 2006;40:63– 69. [CrossRef]
  • 12. Kwak H-S, Lee Y-H, Han Y-M, Jin G-Y, Kim W, Chung G-H. Comparison of renal damage by iodinated contrast or gadolinium in an acute renal failure rat model based on serum creatinine levels and apoptosis degree. J Korean Med Sci 2005;20:841–847. [CrossRef]
  • 13. Wu CT, Weng TI, Chen LP, Chiang CK, Liu SH. Involvement of caspase-12-dependent apoptotic pathway in ionic radiocontrast urografin-induced renal tubular cell injury. Toxicol Appl Pharmacol 2013;266:167–175. [CrossRef]
  • 14. Erley CM, Heyne N, Burgert K, Langanke J, Risler T, Osswald H. Prevention of radiocontrast-induced nephropathy by adenosine antagonists in rats with chronic nitric oxide deficiency. J Am Soc Nephrol 1997;8:1125–1132. https://jasn.asnjournals.org/ content/8/7/1125.long
  • 15. Micili SC, Goker A, Sayin O, Akokay P, Ergur BU. The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats. J Mol Histol 2013;44:339–345. [CrossRef]
  • 16. Ozbilgin K, Karaca F, Turan A, Kose C, Vatansever S, Ozcakir T. The higher heparin binding epidermal growth factor (HB-EGF) in missed abortion. Taiwanese J Obstet Gynecol 2015;54:13–18. [CrossRef]
  • 17. Erbil G, Semerci N, Cilaker Mıcılı S, et al. Immobilization Stress in Pregnancy and the Maternal Brain’s Response: an Experimental Study. J Neurol Sci Turk 2014;31:544–556.
  • 18. Zhao Y, Tao Z, Xu Z, et al. Toxic effects of a high dose of nonionic iodinated contrast media on renal glomerular and aortic endothelial cells in aged rats in vivo. Toxicology Letters 2011;202:253–260. [CrossRef]
  • 19. Adam O, Frost G, Custodis F, et al. Role of Rac1 GTPase activation in atrial fibrillation. J Am Coll Cardiol 2007;50:359–367. [CrossRef]
  • 20. Kawarazaki H, Ando K, Shibata S, et al. Mineralocorticoid receptor --Rac1 activation and oxidative stress play major roles in salt induced hypertension and kidney injury in prepubertal rats. J Hypertension 2012;30:1977–1985. [CrossRef]
  • 21. Zallocchi M, Johnson BM, Meehan DT, Delimont D, Cosgrove D. Alpha1beta1 integrin/Rac1-dependent mesangial invasion of glomerular capillaries in Alport syndrome. Am J Pathol 2013;183:1269–1280. [CrossRef]
  • 22. Antonisamy P, Arasu MV, Dhanasekaran M, et al. Protective effects of trigonelline against indomethacin-induced gastric ulcer in rats and potential underlying mechanisms. Food Funct 2016;7:398–408. [CrossRef]

Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy

Year 2020, Volume: 4 Issue: 3, 230 - 236, 01.09.2020
https://doi.org/10.30621/jbachs.2020.1057

Abstract

Purpose: Radiocontrast-induced nephropathy is an important clinical problem in high-risk patients. The mechanism of cytotoxic effect of radiocontrast media on kidneys is still not fully clarified. Rac1/β-PAK pathway has been shown to play a role in other nephropathies such as diabetic nephropathy. In this study, it was aimed to determine whether Rac1/β-PAK signalling pathway has any role in the development of contrast-induced nephropathy CIN . Methods: Adult male Balb/C mice were used. A single dose of iohexol was given intraperitoneally. Thirty-two mice were divided into 5 groups including control Group1, n=4 , pretreatment Group 2, n=7 , low dose 2 g iodine/kg iohexol Group 3, n=7 , medium dose 2.5 g iodine/kg iohexol Group 4, n=7 and high dose 3 g iodine/kg iohexol Group 5, n=7 . The animals were sacrificed 24 hours after iohexol administration. Nephropathy were evaluated by histological and biochemical analysis methods. Expressions of Rac1 and β-PAK were evaluated by immunohistochemical method. Apoptosis was assessed by TUNEL method. Results: It was found that severity of nephropathy, apoptosis and β-PAK expressions significantly increased as iohexol dosage increased. Although Rac1 expression was higher in the iohexol group in comparison to the control and pretreatment groups, its increase did not show a dose-dependent manner. There was no significant difference among the groups in terms of serum creatinine. Serum cystatin C were increased in all groups compared to the control group, but significant increase was observed only in the pretreatment group. Conclusions: Our findings suggest that the Rac1/β-PAK signal transduction pathway may have a role in the contrast-induced nephropathy model.

References

  • 1. Laville M, Juillard L. Contrast-induced acute kidney injury: how should at-risk patients be identified and managed? J Nephrol 2010;23:387– 398. https://pubmed.ncbi.nlm.nih.gov/20349411/
  • 2. Jeong BY, Lee HY, Park CG, et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 2018;13:e0191034. [CrossRef]
  • 3. Billings FTt, Chen SW, Kim M, et al. alpha2-Adrenergic agonists protect against radiocontrast-induced nephropathy in mice. Am J Physiol Renal Physiol 2008;295:F741–F748. [CrossRef]
  • 4. Wong PCY, Li Z, Guo J, Zhang A. Pathophysiology of contrast-induced nephropathy. Int J Cardiol 2012;158:186–192. [CrossRef]
  • 5. Bedard K, Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 2007;87:245– 313. [CrossRef]
  • 6. Frost JA, Xu S, Hutchison MR, Marcus S, Cobb MH. Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members. Mol Cell Biol 1996;16:3707–3713. [CrossRef]
  • 7. Lee HT, Jan M, Bae SC, et al. A1 adenosine receptor knockout mice are protected against acute radiocontrast nephropathy in vivo. Am J Physiol Renal Physiol 2006;290:F1367–F1375. [CrossRef]
  • 8. Gumustekin M, Micili SC, Arici MA, Karaman M, Guneli ME, Tekmen I. The Effect of Insulin Treatment on Rac1 Expression in Diabetic Kidney. Renal Failure 2013;35:396–402. [CrossRef]
  • 9. Desire L, Bourdin J, Loiseau N, et al. RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitro and in vivo. J Biol Chem 2005;280:37516– 37525. [CrossRef]
  • 10. Evcim AS, Micili SC, Karaman M, et al. The Role of Rac1 on Carbacholinduced Contractile Activity in Detrusor Smooth Muscle from Streptozotocin-induced Diabetic Rats. Basic Clin Pharmacol Toxicol 2015;116:476–84. [CrossRef]
  • 11. Yenicerioglu Y, Yilmaz O, Sarioglu S, et al. Effects of Nacetylcysteine on radiocontrast nephropathy in rats. Scand J Urol Nephrol 2006;40:63– 69. [CrossRef]
  • 12. Kwak H-S, Lee Y-H, Han Y-M, Jin G-Y, Kim W, Chung G-H. Comparison of renal damage by iodinated contrast or gadolinium in an acute renal failure rat model based on serum creatinine levels and apoptosis degree. J Korean Med Sci 2005;20:841–847. [CrossRef]
  • 13. Wu CT, Weng TI, Chen LP, Chiang CK, Liu SH. Involvement of caspase-12-dependent apoptotic pathway in ionic radiocontrast urografin-induced renal tubular cell injury. Toxicol Appl Pharmacol 2013;266:167–175. [CrossRef]
  • 14. Erley CM, Heyne N, Burgert K, Langanke J, Risler T, Osswald H. Prevention of radiocontrast-induced nephropathy by adenosine antagonists in rats with chronic nitric oxide deficiency. J Am Soc Nephrol 1997;8:1125–1132. https://jasn.asnjournals.org/ content/8/7/1125.long
  • 15. Micili SC, Goker A, Sayin O, Akokay P, Ergur BU. The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats. J Mol Histol 2013;44:339–345. [CrossRef]
  • 16. Ozbilgin K, Karaca F, Turan A, Kose C, Vatansever S, Ozcakir T. The higher heparin binding epidermal growth factor (HB-EGF) in missed abortion. Taiwanese J Obstet Gynecol 2015;54:13–18. [CrossRef]
  • 17. Erbil G, Semerci N, Cilaker Mıcılı S, et al. Immobilization Stress in Pregnancy and the Maternal Brain’s Response: an Experimental Study. J Neurol Sci Turk 2014;31:544–556.
  • 18. Zhao Y, Tao Z, Xu Z, et al. Toxic effects of a high dose of nonionic iodinated contrast media on renal glomerular and aortic endothelial cells in aged rats in vivo. Toxicology Letters 2011;202:253–260. [CrossRef]
  • 19. Adam O, Frost G, Custodis F, et al. Role of Rac1 GTPase activation in atrial fibrillation. J Am Coll Cardiol 2007;50:359–367. [CrossRef]
  • 20. Kawarazaki H, Ando K, Shibata S, et al. Mineralocorticoid receptor --Rac1 activation and oxidative stress play major roles in salt induced hypertension and kidney injury in prepubertal rats. J Hypertension 2012;30:1977–1985. [CrossRef]
  • 21. Zallocchi M, Johnson BM, Meehan DT, Delimont D, Cosgrove D. Alpha1beta1 integrin/Rac1-dependent mesangial invasion of glomerular capillaries in Alport syndrome. Am J Pathol 2013;183:1269–1280. [CrossRef]
  • 22. Antonisamy P, Arasu MV, Dhanasekaran M, et al. Protective effects of trigonelline against indomethacin-induced gastric ulcer in rats and potential underlying mechanisms. Food Funct 2016;7:398–408. [CrossRef]
There are 22 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Yasar Aysun Manisaligil This is me

Serap Cilaker Micili This is me

Servet Kizildag This is me

Aslı Celik This is me

Husnu Alper Bagriyanik This is me

Mukaddes Gumustekin This is me

Publication Date September 1, 2020
Published in Issue Year 2020 Volume: 4 Issue: 3

Cite

APA Manisaligil, Y. A., Cilaker Micili, S., Kizildag, S., Celik, A., et al. (2020). Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences, 4(3), 230-236. https://doi.org/10.30621/jbachs.2020.1057
AMA Manisaligil YA, Cilaker Micili S, Kizildag S, Celik A, Bagriyanik HA, Gumustekin M. Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. JBACHS. September 2020;4(3):230-236. doi:10.30621/jbachs.2020.1057
Chicago Manisaligil, Yasar Aysun, Serap Cilaker Micili, Servet Kizildag, Aslı Celik, Husnu Alper Bagriyanik, and Mukaddes Gumustekin. “Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy”. Journal of Basic and Clinical Health Sciences 4, no. 3 (September 2020): 230-36. https://doi.org/10.30621/jbachs.2020.1057.
EndNote Manisaligil YA, Cilaker Micili S, Kizildag S, Celik A, Bagriyanik HA, Gumustekin M (September 1, 2020) Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences 4 3 230–236.
IEEE Y. A. Manisaligil, S. Cilaker Micili, S. Kizildag, A. Celik, H. A. Bagriyanik, and M. Gumustekin, “Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy”, JBACHS, vol. 4, no. 3, pp. 230–236, 2020, doi: 10.30621/jbachs.2020.1057.
ISNAD Manisaligil, Yasar Aysun et al. “Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy”. Journal of Basic and Clinical Health Sciences 4/3 (September 2020), 230-236. https://doi.org/10.30621/jbachs.2020.1057.
JAMA Manisaligil YA, Cilaker Micili S, Kizildag S, Celik A, Bagriyanik HA, Gumustekin M. Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. JBACHS. 2020;4:230–236.
MLA Manisaligil, Yasar Aysun et al. “Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy”. Journal of Basic and Clinical Health Sciences, vol. 4, no. 3, 2020, pp. 230-6, doi:10.30621/jbachs.2020.1057.
Vancouver Manisaligil YA, Cilaker Micili S, Kizildag S, Celik A, Bagriyanik HA, Gumustekin M. Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. JBACHS. 2020;4(3):230-6.