Labile hemoglobin A1c: A factor affecting the estimation of glycated hemoglobin

Year 2017, Volume: 8 Issue: 4, 124 - 126, 29.12.2017

Kalpana Singh , Bhawna Mahajan Shweta Singh Abbas Ali Mahdi

https://doi.org/10.5799/jcei.382433

Abstract

Labile hemoglobin A1c
(also known as pre-HbA1c or LA1c or pre-glycohemoglobin) an unstable form, is a
Schiff base formed during non-enzymatic glycation of hemoglobin. The
concentration of labile fraction varies with acute change in plasma glucose
level. Labile hemoglobin A1c fraction cannot be separated from the stable
ketoamine fraction by most of the methods available for estimation of glycated
hemoglobin (HbA1c). Though ion-exchange HPLC (high pressure liquid
chromatography) method can separate the labile HbA1c fraction, but in some
situation abnormally high level of this fraction may serve as a pre-analytical
error interfering in estimation of glycated hemoglobin. By focusing on the
hemoglobin chromatogram, reporting of this pre-analytical error can be
minimized in HPLC method.

Keywords

Diabetes, Glycated hemoglobin, HPLC, Labile hemoglobin

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