j cell neurosci oxid stress

Journal of Cellular Neuroscience and Oxidative Stress

2149-7222

Yasemin NAZIROĞLU

10.37212/jcnos.809635

Neurosciences

Sinirbilim

Clostridium botulinum neurotoxin A inhibits DBTRG glioblastoma cell proliferation and TRPV1 channel signaling pathways

Akyuva

Yener

MUSTAFA KEMAL UNIVERSITY

10 12 2020

12 1 903 913 12 27 2019 03 30 2020

2008

Journal of Cellular Neuroscience and Oxidative Stress

Prevalence of glioblastomas is high within the adult brain tumors and the proliferation of the glioblastomas was induced by excessive Ca2+ influx. Ca2+ permeable TRPV1 channel is gated by capsaicin and reactive oxygen species (ROS), although its activity was decreased in neurons by AMG and antioxidants. Clostridiumbotulinum neurotoxin A (BotxA) acted antioxidant action in several cells and its treatment modulated TRPV1 in neurons. Hence, treatment of BotxA may modulateglioblastoma cell proliferation and death via inhibition of TRPV1 in the DBTRG glioblastoma in vitro cell line model. The DBTRG cells were divided into three groups as control, BotxA (5 IU for 24 hours) and BotxA+TRPV1 channel blocker (AMG and 1 µM for 30 min). Intracellular Ca2+ response to TRPV1 activation was increased in the cells from capsaicin, although it was reduced by the BotxA and AMG. BotxA treatment decreased cell proliferation, although its treatment increased cell death (propidium iodide/Hoechst rate). In addition, BotxA decreased mitochondrial membrane depolarization levels, cytosolic and mitochondrial ROS generation in the cells. Their levels were further decreased in the BotxA+AMG group by the AMG treatment. The antiproliferative and neurotoxic effects of BotxA were shown to be exerted via modulation of oxidative stress and TRPV1 activation. BotxA could be used as an effective agent in the treatment of glioblastoma proliferation.

Glioblastoma Mitochondria Oxidative stress Proliferation TRPV1 channel

BSN Health, Analyses, Innovation, Consultancy, Organization, Agriculture, Industry and Trade Limited Company (Göller Bölgesi Teknokenti, Isparta, Turkey).

2018-32

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