Pistacia Türlerinin rbcL Kloroplast Gen Bölgesi ile Filogenetik Analizi

Year 2023, Volume: 35 Issue: 3, 359 - 367, 30.09.2023

Ezgi Çabuk Şahin

https://doi.org/10.7240/jeps.1273064

Abstract

Pistacia cinsindeki beş türün filogenisi, plastid rbcL gen bölgesi kullanılarak değerlendirilmiştir. Yaprak dokusundan genomik DNA izole edilmiş ve rbcL gen bölgesi primeriyle PZR analizi ve DNA dizilemesi yapılmıştır. Elde edilen dizi verileri kullanılarak Pistacia türleri arasındaki filogenetik ilişkileri ortaya çıkarmak için filogenetik ve PCoA (Principle Component Analysis) analizleri gerçekleştirilmiştir. Filogenetik analiz sonucuna göre, P. vera, P. khinjuk ve P. atlantica arasında; P. palaestina ve P. terebinthus arasında dallarda daha yakın gruplaşma gözlenmiştir. PCoA sonuçları filogenetik analiz sonucunu desteklemiştir. Sonuç olarak, rbcL gen bölgesi tür bazlı gruplandırmada güçlü bulunmuştur. Kloroplast gen bölgesi dizi bilgisi, yerel fıstık gen havuzu üzerinde yürütülecek ıslah programları kapsamında hazırlanacak moleküler bir veri tabanı için güvenilirdir.

Keywords

Pistacia, kloroplast geni, rbcL, filogeni

Phylogenetic Analysis of Pistacia Species with rbcL Chloroplast Gene Region

Abstract

The phylogeny of five species in the genus Pistacia was assessed using the plastid rbcL gene region. Genomic DNA was isolated from leaf tissue and PCR analysis with primer of rbcL gene region and DNA sequencing were performed. Using obtained sequence data, phylogenetic and PCoA (Principle Component Analysis) analyses were performed for reveal the phylogenetic relationships among Pistacia species. According to the result of phylogenetic analysis, closer grouping in clades was observed between P. vera; P. khinjuk and P. atlantica; between P. palaestina and P. terebinthus. Result of PCoA analysis supported the result of phylogenetic analysis. As a result, rbcL gene region was found powerful at species-based grouping. Revealed sequence information of chloroplast gene region is reliable to elaborate a molecular database to conduct breeding programs on local pistachio gene pool.

Keywords

Pistacia, chloroplast gene, rbcL, phylogeny

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