j. fac. pharm. ankara Journal of Faculty of Pharmacy of Ankara University 1015-3918 2564-6524 Ankara University Pharmaceutical Analytical Chemistry Eczacılıkta Analitik Kimya ASTERISCUS GRAVEOLENS'TEN İZOLE EDİLEN METİL GALAT VE SİRİNGİK ASİDİN FUSARIUM OXYSPORUM F. SP. ALBEDINIS’E KARŞI ANTİFUNGAL AKTİVİTESİ ANTIFUNGAL ACTIVITY OF METHYL GALLATE AND SYRINGIC ACID ISOLATED FROM ASTERISCUS GRAVEOLENS AGAINST FUSARIUM OXYSPORUM F. SP. ALBEDINIS https://orcid.org/0000-0001-6158-3957 Belhi Zeyneb Tahri Mohamed University, Phytochemistry and Organic Synthesis Laboratory https://orcid.org/0009-0002-4861-3183 Roucham Zahira Tahri Mohamed University, Phytochemistry and Organic Synthesis Laboratory https://orcid.org/0000-0001-8977-1888 Boulenouar Noureddine Phytochemistry and Organic Synthesis Laboratory, Tahri Mohamed University, 08000, Bechar, ALGERIA https://orcid.org/0000-0002-4972-702X Cheriti Abdelkrim Tahri Mohamed University, Phytochemistry and Organic Synthesis Laboratory 11 25 2024 48 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023) 1303 1312 02 24 2024 06 12 2024 Copyright © 1971, Journal of Faculty of Pharmacy of Ankara University 1971 Journal of Faculty of Pharmacy of Ankara University

Amaç: Bu çalışmanın amacı, Asteriscus graveolens toprak üstü kısımları ekstresinden Fusarium oxysporum f. sp. albedinis'e (Foa) karşı antifungal aktiviteden sorumlu bileşikleri izole etmek ve tanımlamak ve Fusarium solgunluğunun kontrolü için seçilen bileşiklerin in vitro etkilerini değerlendirmektir. Gereç ve Yöntem: Asteriscus graveolens'te fenolik bileşiklerin varlığını ortaya koyduk ve bu bileşiklerden elde edilen toprak üstü kısım ekstrelerinin antifungal aktiviteleri doğrudan biyootografi ile Fusarium oxysporum f. sp. albedinis'e (Foa) karşı misel büyümesi üzerindeki etkileri açısından araştırıldı. Antifungal bileşikler silika jel kolon kromatografisi ve ince tabaka kromatografisi kullanılarak A. graveolens ekstresinden izole edilmiştir. Antifungal bileşiklerin yapısal tanımlaması NMR (1H ve 13C) spektrofotometrisi[A1] ve LC-MS/MS[A2] kullanılarak yapılmıştır.Sonuç ve Tartışma: İzole edilen bileşikler, spektral ve fiziksel verilerinin literatürle karşılaştırılmasına dayanarak metil gallat (MG) ve siringik asit (SA) olarak tanımlanmıştır.

Objective: The objective of this study was to isolate and identify the compounds responsible for the antifungal activity against Fusarium oxysporum f. sp. albedinis (Foa) from Asteriscus graveolens aerial parts extract, and to evaluate the effects in vitro of selected compounds for control of Fusarium wilt.Material and Method: We reveal the presence of the phenolic compounds in Asteriscus graveolens, from which the antifungal activities of aerial parts extracts were investigated for effects on the growth of mycelia against Fusarium oxysporum f. sp. albedinis (Foa) by direct bioautography. The antifungal compounds were isolated from A. graveolens extract using silica gel column chromatography and thin-layer chromatography. Structural identification of the antifungal compounds was conducted using NMR (1H and 13C) spectrophotometry and LC-MS.Result and Discussion: The isolated compounds were identified as methyl gallate (MG) and syringic acid (SA) based on comparing their spectral and physical data with the literature.

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