j. fac. pharm. ankara Journal of Faculty of Pharmacy of Ankara University 1015-3918 2564-6524 Ankara University 10.33483/jfpau.1659355 Toxicology Toksikoloji SIÇAN MİKROZOMLARINDA CYP1A1 AKTİVİTESİNİN CİTRUS L. MEYVE SULARI İLE MODÜLASYONU MODULATION OF CYP1A1 ACTIVITY IN RAT MICROSOMES BY CITRUS L. FRUIT JUICES https://orcid.org/0000-0003-0803-2886 Gökkaya İçim KARADENİZ TEKNİK ÜNİVERSİTESİ https://orcid.org/0000-0002-0859-1760 Koçyiğit Ayşegül ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK PR. https://orcid.org/0000-0002-0043-4808 Güven Aksu Naile Merve ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ https://orcid.org/0000-0001-6323-0338 Renda Gülin KARADENİZ TEKNİK ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK MESLEK BİLİMLERİ BÖLÜMÜ, FARMAKOGNOZİ ANABİLİM DALI https://orcid.org/0000-0001-9817-9034 Can Eke Benay ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ 01 14 2026 50 1 38 44 03 17 2025 06 10 2025 Copyright © 1971, Journal of Faculty of Pharmacy of Ankara University 1971 Journal of Faculty of Pharmacy of Ankara University

Amaç: Citrus L. meyve suları, küresel meyve suyu pazarının yaklaşık üçte ikisini oluşturan diyette yaygın olarak tüketilen içeceklerden biridir. Meyve sularının tıbbi ilaçlarla eş zamanlı tüketilmesi bitki-ilaç etkileşim riskini ortaya çıkarmaktadır. Önceki çalışmalarda C. paradisi, C. aurantifolia, C. maxima ve C. sinensis meyve sularının sitokrom (CYP)1A1 aktivitesini etkilediği belirtilmiştir. C. australasica, C. limon, C. reticulata × C. sinensis ve C. japonica meyve sularının CYP1A1 aktivitesi üzerindeki etkisini değerlendiren bir çalışma bulunmamaktadır. Gereç ve Yöntem: Bu çalışmada, narenciye meyve sularının sıçan karaciğer dokusu üzerindeki CYP1A1 aktivitesini 7-etoksirezorufin O-deetilaz yöntemi kullanarak değerlendirilmiştir. Sonuç ve Tartışma: Çalışılan altı meyve suyu arasında C. sinensis, C. japonica ve C. australasica'nın CYP1A1 aktivitesini sırasıyla %50, %50 ve %60 oranında inhibe ettiği bulunmuştur. Bu meyve suları, CYP 1A1 izoenzimi tarafından metabolize olan ilaçlar alınırken dikkatli kullanılmalıdır.

Objectives: Juices prepared from citrus fruits make up almost two-thirds of the worldwide market for juice, which makes them one of the most common beverages in the diet. The simultaneous consumption of fruit juices and medicinal drugs poses a risk of herb-drug interactions. Previous studies have noted that Citrus paradisi Macfad., Citrus aurantifolia Linn., Citrus maxima (Burm) Merrill, and Citrus sinensis (L.) Osbeck juices affect cytochrome (CYP) 1A1 activity. Studies testing the effects of Citrus australasica F. Muell., Citrus limon (L.) Osbeck, Citrus reticulata Blanco× Citrus sinensis (L.) Osbeck, and Citrus japonica Thunb. fruit juices on CYP1A1 activity are lacking. Material and Methods: This study evaluate the CYP1A1 activity of Citrus fruit juices on rat liver tissues using the 7-ethoxyresorufin O-deethylase (EROD) assay. Result and Discussion: Among the six fruit juices studied, C. sinensis, C. japonica and C. australasica were found to inhibit CYP1A1 activity by 50%, 50% and 60%, respectively. These juices should be used with caution when taking medicines metabolized by the CYP1A1 isoenzyme.

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