PROTECTIVE ROLE OF CRATAEGUS MONOGYNA ON SPERM QUALITY AND TESTIS OXIDATIVE STRESS AGAINST COPPER-INDUCED TOXICITY

Year 2020, Volume: 44 Issue: 3, 452 - 469, 30.09.2020

Feriel Remıta , Cherif Abdennour Amina Talbı , Kamel Khelılı

https://doi.org/10.33483/jfpau.716103

Cited By: 3

Abstract

Objective: The purpose of this study was to investigate the possible beneficial use of common hawthorn Crataegus monogyna aqueous extract at 1.5g/Kg bw/day against chronic copper sulfate intoxication (100mg/Kg bw) in Wistar rats.

Material and Method: Animals were divided into 6 groups; the untreated control (C), 2 positive controls treated respectively with hawthorn fruits (F) and leaves (L), 1 group treated with copper (Cu) and 2 combined treatment groups treated with Cu and hawthorn-fruits (CuF) and Cu and hawthorn-leaves (CuL). After 30 days of oral administration, testis weight and plasma testosterone levels were evaluated, in addition to the epididymal sperm concentration, motility, vitality, velocity (VCL, VSL and VAP), the amplitude of lateral head displacement (ALH), and the beat cross frequency (BCF). Testicular glutathione (GSH), malondialdehyde (MDA), and glutathione peroxidase (GPx) were also evaluated.

Result and Discussion: Cu exposure reduced testosterone, sperm concentration, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx levels compared to control groups. Dead sperm and MDA levels were increased in rats of Cu group compared to the untreated control. When compared to the Cu group, levels of testosterone, sperm concentration, sperm motility, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx were much higher in the CuF and CuL groups, along with a significantly lower MDA concentration. In conclusion, hawthorn, when co-administered as an aqueous extract with Cu, protected most biological markers against copper toxicit, while positive control (s) boosted sperm concentration and velocity (VCL and VAP).

Keywords

C. monogyna, CASA, Copper, sperm, testosterone

Supporting Institution

The General Directorate of Scientific Research and Technological Development (DGRSDT)

Project Number

number 06/2016, recipient C. ABDENNOUR

Thanks

Authors would like to thank The General Directorate of Scientific Research and Technological Development (DGRSDT) for financial support (Award number 06/2016, recipient C. ABDENNOUR). Thanks are also given to Pasteur Institute (Algiers) for providing rats.

CRATAEGUS MONOGYNA’NIN, BAKIR KAYNAKLI TOKSİSİTEYE KARŞI SPERM KALİTESİ VE TESTİS OKSİDATİF STRESİ ÜZERİNDEKİ KORUYUCU ROLÜ

Abstract

Amaç: Bu çalışmanın amacı, Wistar sıçanlarında, kronik bakır sülfat zehirlenmesine (100mg / Kg bw) karşı yaygın alıç Crataegus monogyna sulu ekstraktının (1.5g / Kg bw/ gün) olası faydalı kullanımını araştırmaktır.

Gereç ve Yöntem: Hayvanlar, tedavi edilmemiş kontrol (C), sırasıyla alıç meyveleri (F) ve yaprakları (L) ile muamele edilmiş 2 pozitif kontrol, bakır (Cu) ile muamele edilmiş grup ve Cu ve alıç meyveleri (CuF) ve Cu ve alıç yaprakları (CuL) ile muamele edilmiş 2 kombine tedavi grubu olmak üzere 6 gruba ayrılmıştır. 30 günlük oral uygulamadan sonra, epididimal sperm konsantrasyonu, motilite, canlılık, hız (VCL, VSL ve VAP), dış yana baş deplasmanının amplitüdü (ALH) ve sperm kuyruğu vuruş sıklığının (BCF) yanısıra testis ağırlığı ve plazma testosteron seviyeleri değerlendirilmiştir. Ayrıca, testis glutatyonu (GSH), malondialdehid (MDA) ve glutatyonperoksidaz (GPx) da değerlendirilmiştir.

Sonuç ve Tartışma: Cu maruziyeti, kontrol gruplarına kıyasla testosteron, sperm konsantrasyonu, canlı sperm, VCL, VSL, VAP, ALH, BCF, GSH ve GPx seviyelerini azaltmıştır. Cu grubunun sıçanlarında ölü sperm ve MDA seviyeleri, tedavi edilmeyen kontrole kıyasla artmıştır. Cu grubuyla karşılaştırıldığında, testosteron seviyeleri, sperm konsantrasyonu, sperm motilitesi, canlı sperm, VCL, VSL, VAP, ALH, BCF, GSH ve GPx seviyeleri CuF ve CuL gruplarında çok daha yüksek ve MDA konsantrasyonları ise anlamlı olarak düşüktür. Sonuç olarak, alıç, Cu ile sulu bir özüt olarak uygulandığında, bakır toksisitesine karşı çoğu biyolojik belirteci korumuş ve sperm konsantrasyonunu ve hızını (VCL ve VAP) artırmıştır

Keywords

Bakır, C. monogyna, CASA, sperm, testosteron

Project Number

number 06/2016, recipient C. ABDENNOUR

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