j health sci med / jhsm Journal of Health Sciences and Medicine 2636-8579 MediHealth Academy Yayıncılık 10.32322/jhsm.1129894 Health Care Administration Sağlık Kurumları Yönetimi Identification of lymphocyte subgroups with flow cytometry in COVID-19 patients https://orcid.org/0000-0003-3312-8476 Berber İlhami İNÖNÜ ÜNİVERSİTESİ https://orcid.org/0000-0001-8634-2543 Kırıcı Berber Nurcan TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ https://orcid.org/0000-0002-5916-0119 Sarıcı Ahmet TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ https://orcid.org/0000-0003-1208-4072 Gözükara Bağ Harika INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0001-7498-344X Biçim Soykan INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0003-2902-9328 Turgut Burhan NAMIK KEMAL UNIVERSITY, SCHOOL OF MEDICINE https://orcid.org/0000-0002-2538-857X Çağan Furkan INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0002-3285-417X Erkurt Mehmet Ali INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0002-5581-8104 Uysal Ayşe FIRAT UNIVERSITY, SCHOOL OF MEDICINE https://orcid.org/0000-0001-5768-9863 Ulutaş Nihal Sümeyye INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0001-8605-8497 Kaya Emin INONU UNIVERSITY, FACULTY OF MEDICINE https://orcid.org/0000-0001-6126-0816 Kuku İrfan INONU UNIVERSITY, FACULTY OF MEDICINE 07 20 2022 5 4 1183 1189 06 13 2022 06 29 2022 Copyright © 2018, Journal of Health Sciences and Medicine 2018 Journal of Health Sciences and Medicine

Objective: We aimed to determine lymphocyte subgroups and activation status of flow cytometry in COVID-19 patients and examine their relationship with disease stage and length of hospital stay.Material and Method: Forty patients were analyzed in this study and compared with the age and sex-matched 40 healthy controls. COVID-19 patients have split as early and advanced-stage diseases. Flow cytometry assay was performed to determine the counts of lymphocyte subsets and activation status. Total lymphocyte count was calculated and CD45 (cluster of differentiation), CD3, CD4, CD8, CD19, CD27, CD38, CD56, CD57, and IgD were studied on lymphocyte gate. T helper / T cytotoxic rates and length of hospital stay were recorded.Results: The patients' CD3(+)CD4(+) ( T helper) count and CD27 expression on T cells counts were significantly lower, and CD57 expression on CD3(+)CD8(+) T cytotoxic cells were significantly higher (p

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