j health sci med / jhsm

Journal of Health Sciences and Medicine

2636-8579

MediHealth Academy Yayıncılık

10.32322/jhsm.605654

Health Care Administration

Sağlık Kurumları Yönetimi

HIV pozitif hastalarda HHV-6 prevalansı ve varyant tiplerinin belirlenmesi

The prevalence of HHV-6 and variant type in HIV positive patients

https://orcid.org/0000-0002-1019-0798

Tarhan

Gülnur

Adıyaman Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Adıyaman

https://orcid.org/0000-0001-9027-079X

Yılmaz

Neziha

Yozgat Bozok Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı,Yozgat Cesur

Salih

Ankara Eğitim ve Araştırma Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Ankara

01 15 2020

3 1 12 15 08 16 2019 11 01 2019

2018

Journal of Health Sciences and Medicine

Amaç: Bu çalışmanın amacı HIV enfeksiyonu pozitifsaptanan hastalarda HHV-6 DNA sıklığının, HHV-6 DNA’sı pozitif saptananolgularda ise HHV-6 varyant türünün (varyant A veya varyant B) belirlenmesiydi.Gereçve Yöntem: Çalışmaya 20 HIV pozitif hastaile 57 HIV negatif sağlıklı birey dahil edildi. Hasta ve kontrol grubunda HHV-6DNA’sı polimeraz zincir reaksiyonu (PZR) ile araştırıldı. HHV-6 DNA pozitifsaptanan olgularda HHV-6 varyant türü restriksiyon parça uzunluğu polimorfizmi (restrictionfragment lenght polymorphisim: RFLP) ilearaştırıldı. İstatistiksel değerlendirme SPSS programında yapıldı, p≤0.05istatistiksel olarak anlamlı kabul edildi. Bulgular: HIV pozitif hastalarda HHV-6 DNA pozitifliği %45(9/20), HIV negatiflerde ise %33,3 (19/57) olarak belirlendi. Toplamdapozitiflik oranı %36,3 (28/77) idi. HIV pozitif hastalarda HHV-6 varyant Bsaptanırken, varyant A saptanmadı. Sonuç: HIV pozitif hastalarda HHV-6 seropozitifliği vevaryant tiplerinin öneminin belirlenmesi için daha fazla sayıda olgu içeren başkaklinik çalışmalara gereksinim olduğu görüşündeyiz.

Objective: The aim of thisstudy was to determine the frequency of HHV-6 DNA in patients with HIV positiveinfection and HHV-6 variant type (variant A or variant B) in patients with HHV-6DNA positive Material and Method: TwentyHIV-positive patients and 57 HIV negative healthy subjects were included in thestudy. HHV-6 DNA was investigated by polymerase chain reaction (PCR) in thepatient and control groups. In cases with HHV-6 DNA positive, HHV-6 variant wasinvestigated with restriction fragment length polymorphism (RFLP). Statisticalanalysis was performed using SPSS program, p≤0.05 was considered statisticallysignificant.Results: HHV-6 DNA positivity was foundto be 45% (9/20) in HIV positive patients and 33.3% (19/57) in HIV negativepatients. Overall, the positivity rate was 36.3% (28/77). HHV-6 variant B wasdetected in HIV positive patients, but variant A was not.Conclusion: We believe thatfurther clinical studies involving more cases are needed to determine theimportance of HHV-6 seropositivity and variant types in HIV positive patients.

HIV HHV-6 varyant A varyant B koenfeksiyon

HIV HHV-6 varyant A varyant B coinfection

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