j immunol clin microbiol

Journal of Immunology and Clinical Microbiology

2528-9470

Erkan YULA

10.5455/jicm.7.20160520

Clinical Sciences

Klinik Tıp Bilimleri

The distribution of Rotavirus G and P genotypes in children with acute gastroenteritis in Cukurova region, Turkey

Yarkın

Fugen

06 30 2016

1 1 3 9 04 18 2016 04 21 2016

2016

Journal of Immunology and Clinical Microbiology

Background: Rotavirusis the major cause of acute gastroenteritis in infants and young childrenworldwide. The aim of the study was to determine the frequency of rotavirusinfection and the distribution of rotavirus G and P genotype combination amongchildren under 5 years of age with acute gastroenteritis in Cukurova region,Turkey, between October 2009 and June 2010.Material and Methods: The stool specimens (n=846) collected from children withacute gastroenteritis were analyzed by enzyme-linked immunosorbent assay(ELISA) for group A rotavirus antigen. Semi-nested multiplex reversetranscription-polymerase chain reaction (RT-PCR) test was performed forrotavirus G and P genotyping. Results: Therate of rotavirus infection was found to be in 144 patients (17%). Thepredominant rotavirus genotype was G1P[8] (22.2%), followed by G1P[4] (17.3%),G2P[4] (13.8%), G9P[4] (6.3%), G9P[8] (4.8%), G2P[8] (2.8%), G1P[10] (2.1%) andG4P[8] (1.4%). The most common G genotype was G1 (41.7%), followed by G2 (16.6%),G9 (11.1%) and G4 (1.4%). Rotavirus P[4] genotype was identified in 37.5%, P[8]in 31.2% and P[10] in 2.1% of samples. The prevalence of mixed rotavirusinfections was 29.2% (n=42).Conclusion: Althoughthe predominant rotavirus genotypes circulating during the study period in ourregion are targets of current rotavirus vaccines, uncommon, non-vaccinerotavirus genotype combinations such as G1P[4]and G9P[4], which might appear to be the result of mixed rotavirusinfections with high rate (29.2%), were also detected. G1 is included in bothrecent rotavirus vaccines. The continuous investigation of molecularepidemiology of rotavirus infections is essential to evaluate the effectivenessof rotavirus vaccines.

Rotavirus Gastroenteritis Genotypes ELISA RT-PCR

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