Comparative study for production and charcterization of D-Xylanase produced from locally isolate Aspergillus niger by submerged and solid state fermentation

Year 2016, Volume: 11 Issue: 2, 189 - 197, 30.06.2016

Ayat Adnan Abbas

Abstract

Xylanase was pooled from Aspergillus
niger that was cultivated on czepak dox with addition rice husk. Condition affecting
xylanase production were assessed these included p H, temperature of
cultivation, carbon source, nitrogen source, incubation period, and inoculums
size for both types of culture. Results obtained were as follow ion exchange
DEAD-Cellulose specific activity from solid state fermentation (І, ІІ) were (902.43,3666.6 U/mg),
with yield (0.602,0.212%) and No. of fold (1.24,5.034). The specific
activity from submerged fermentation (P 1, P2) were (1861.76,910 U/mg), with yield (1.033, 0.531%) and No. of fold
(5.570, 2.790). the optimum and stability p H for
enzyme activity type (ІІ) produced by solid state fermentation
(7.0 and 7.0) respectively. the optimum and stability temperature for enzyme type (ІІ) produced by solid state fermentation
50°C and 70 °C respectively. and the optimum and stability p H for enzyme activity type (P1) produced by
submerged (7.0 and 7.0) respectively. the optimum and stability
temperature for enzyme activity type
(P1) produced by submerged fermentation 30
and 60 °C respectively.

Keywords

xylanase, solid state fermentation, purification, submerged fermentatio

References

• Subramaniyan S, Prema P, (2002) Biotechnology of microbial xylanases, enzymology, molecular biology and application. Crit. Rev. Biotechnol. 22, 33-64.
• Collins T, Gerday C, Feller G, (2005) Xylanases, xylanase families and extremophilic xylanases, FEMS Microbiol. Reviews, 29: 3-23. Kulkarni N, Shendye A, Rao M, (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiol. Rev. 23, 411-56.
• Haltrich D, Nidetzky B, Kulbe KD, Steiner W, Zupancic S, (1996) Production of fungal xylanases. Biores. Technol. 58: 137-161.
• Pandey A, Selvakumar P, Soccol CR, Nigram P, (1999) Solid state fermentation for the production of industrial enzymes. Curr. Sci. 77:149-62.
• Salles BC, Medeiros RG, Bao SN, Silva Jr, FG, Filho EXF, (2005) Effectof cellulase-free xylanases from Acrophialophora nainiana and Humicola grisea var. thermoidea on eucalyptus kraft pulp. Process Biochem. 40: 343 – 349.
• Nakamura S, Wakabayashi K, Nakai R, Aono R., Horikoshi K, (1993) Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. Strain 41 M-1. App. Environ. Microbiol. 59, 2311-2316
• Bailey MJ, Beily P,Poutanen K, (1992) Interlaboratory testing and methods for assay of xylanase activity. J. Biotechnol. 23: 257-70.
• Miller GL, (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-8.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, (1951) Protein measurement with the Folin Phenol regent. J. Gen. Microbiol. 131, 3017-27.
• Malarvizhi K, Murugesan K, Kalaichelvan PT, (2003) Xylanase production by Ganoderma lucidum on liquid and solid state fermentation. Ind. J. Exp. Biol. 41, 620-626.
• Javier D B, Faustino S, Mario D B, Guillermo RC, and Rajni H. K,. (1998) Purification and characterization of a thermostable xylanase from Bacillusamyloliquefaciens. Enzyme & Microbial Techn. 22 (1): 42-49.
• Marta C. T.D, Ana C A P, Edilberto P P, Alexandre N P,and Telma T F, (2000) Characterization of alkaline xylanases from Bacillus pumilus. Brazilian Journal of Microbiology. 31: 90-94.
• Gawande PV, Kamat MY, (1999) Production of Aspergillus xylanase by lignocellulosic waste fermentation and its application. J. Appl. Microbiol. 87: 511-519.
• Abdel-Sater MA, El-Said AHM, (2001) Xylan decomposing fungi and xylanolytic activity in agricultural and industrial wastes. Inter. Biodet. Biodeg. 47: 15-21.
• Suprabha G, Nair, Sindhu R, Shankar Shashidhar, (2008) Fungal xylanase production under solid state and submerged fermentation conditions. African J Microbi. Research. 2: 082-086.
• Ghosh A, Das A.K, Mishra G, Nanda,(1993) Aspergillus sydowii MG 49 is a strong producer of thermostable xylanolytic enzymes, Enzyme Microb. Technol. 15 703–779.
• Bindu B, Jitender S, Saurabh SD, Ramesh CK, (2007) Enhanced production of cellulose- free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry. Enzyme & Microbial Techn. 41, 733-739
• Silas B. C, Busiswa G, Ignatious, N, Elbert J. V. R., Andrew C and Emil K.A, (2008) Production, purification and characterization of celullase-free xylanase from Aspergillus terreus UL 4209. African J. Biotech. 7, 3939-3948.
• Sapre M, Jha, H. and Patil M, (2005) Purification and characterization of a thermoalkalophilic xylanase from Bacillus sp. World Journal of Microbiology and Biotechnology. 21, 649-654.
• Júlio X. H, Luís H. B. S, Plinho F. H, and Marco A. Z. A, (2006) Purification and properties of a xylanase produced by Bacillus circulans BL53 on solid-state cultivation. Biochemical Engineering J. 32, 179-184.
• Winterhalter and W. Liebl, (1995) Two extremely thermostable xylanases of the hyperthermophilic bacterium Thermotoga maritima MSB8, Appl. Environ. Microbiol. 61, 1810-1815.
• Zverlov K, Piotukh O, Dakhova G, Velikodvorskaya R, Borriss, (1996) The multidomain xylanase A of the hyperthermophilic bacterium Thermotoga neapolitana is extremely thermoresistant. App. Microbiol. Biotechnol, 45, 245-247.