Enterotoxemia in Albanian Zoo-Park Llama (Lama glama): Clostridium perfringens-type C was the Causative Agent

Year 2016, Volume: 11 Issue: 1, 42 - 46, 30.03.2016

Sulo Kotorri Kastriot Korro Rigers Bakiu Pellumb Muhedini Besa Gora

Abstract

Clostridium perfringens causes enteric diseases in mammals, usually
called enterotoxemia. C. perfringens was
isolated from ileum and jejunum of dead llamas in one of zoo-parks in Tirana.
Diagnosis of the disease was based on clinical sings, pathologically and also
in isolation of the causes. Isolates colonies in agar blood were like dewdrops,
smooth, gray, shiny and convex. In microscopic preparations colored and
prepared with Gram technique were visible small bacillus, in sticks shape and
Gram positive. Biochemical identification with API 20A kit valued isolates
catalase and lecithinase positive, with hemolytic activity in agarblood from
sheep with a dual zone of hemolysis. Isolates fermented producing gas and acid
toward glucose, fructose lactose, sucrose and mannitol. Biological test of
neutralization in white mice with antitoxins α and β, determined type C of C. perfringensas cause of llamas death,
a result that was confirmed by ELISA kit. Besides treatment with drugs for
pre-protection of llamas was used bivalent killed vaccine with types C and D of
Clostridium perfringens.

Keywords

Enterotoxemia, C.perfringens, API 20A, biological test of neutralization, ELISA, llamas

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