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The Role of Curcumin-Loaded Solid Lipid Nanoparticles Labeled with Tc-99m in the Liver-Spleen Scintigraphy

Year 2016, Volume: 6 Issue: 3, 27 - 34, 30.09.2016

Abstract

Recently, nanotechnology and molecular imaging felds are integrated to facilitate the diagnosis and
the treatment of diseases with create multi-functional nanoparticles. Nuclear medicine is one of the felds that beneft
from the multi-faceted and unique characteristics of new generation imaging agents that are made of nanoparticles.
This study investigated the feasibility of using curcumin-loaded SLNs in liver and spleen scintigraphy. Initially, the
curcumin-loaded solid lipid nanoparticles (C-SLN) were prepared using microemulsion and solidifcation at low
temperatures. The size of the C-SLN particle is measured by Laser diffraction (LD) method. Moreover, by applying
thermogravimetric analysis (TGA) it is observed that C-SLN particles are stable with temperature variation. Then,
they are radiolabeled with Technetium-99m (
99mTc). Using the thin-layer chromatography method, the radiolabeling
effciency was found to be higher than 95%.
99mTc-C-SLNs were injected to the rabbit intravenously (i.v.) and
using a gamma camera, dynamic images were recorded. Liver and spleen were clearly displayed in these dynamic
images. In addition to scintigraphic fndings, the presence of
99mTc-C-SLNs in the liver and the spleen was observed
in the ex vivo biodistribution studies and the analyses performed under the fluorescence microscope.
  

References

  • Aburahma MH, Badr-Eldin SM, 2014. Compritol 888 ATO: a multifunctional lipid excipient in drug delivery systems and nanopharmaceuticals. Expert Opin Drug Deliv, 11: 1865-83.
  • Aggarwal BB,Harikumar KB, 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol, 41: 40-59.
  • Andreozzi E, Seo JW, Ferrara K, Louie A, 2011, Novel method to label solid lipid nanoparticles with 64cu for positron emission tomography imaging. Bioconjug Chem, 22: 808-18.
  • Anwar M, Asfer M, Prajapati AP, Mohapatra S, Akhter S, Ali A, Ahmad, FJ, 2014. Synthesis and in vitro localization study of curcumin-loaded SPIONs in a micro capillary for simulating a targeted drug delivery system. Int J Pharm, 468: 158-64.
  • Banerjee I, De K, Chattopadhyay S, Bandyopadhyay AK, Misra M, 2014. An easy and effective method for radiolabelling of solid lipid nanoparticles. J Radioanal Nucl Chem, 302:837–843.
  • Gao Y, Li Z, Sun M, Guo C, Yu A, Xi Y, Cui J, Lou H, Zhai G, 2011. Preparation and characterization of intravenously injectable curcumin nanosuspension. Drug Deliv, 18: 131-42.
  • Kakkar V, Mishra AK, Chuttani K, Kaur IP, 2013. Proof of concept studies to confrm the delivery of curcumin loaded solid lipid nanoparticles (C-SLNs) to brain. Int J Pharm, 448: 354-9.
  • Kakkar V, Muppu SK, Chopra K, Kaur IP, 2013. Curcumin loaded solid lipid nanoparticles: an effcient formulation approach for cerebral ischemic reperfusion injury in rats. Eur J Pharm Biopharm, 85: 339-45.
  • Kathe N, Henriksen B, Chauhan H, 2014. Physicochemical characterization techniques for solid lipid nanoparticles: principles and limitations. Drug Dev Ind Pharm, 40: 1565-75.
  • Kumar SS, Mahesh A, Mahadevan S, Mandal AB, 2014. Synthesis and characterization of curcumin loaded polymer/lipid based nanoparticles and evaluation of their antitumor effects on MCF-7 cells. Biochim Biophys Acta, 1840: 1913-22.
  • Li B, Konecke S, Wegiel LA Taylor, LS, Edgar KJ, 2013. Both solubility and chemical stability of curcumin are enhanced by solid dispersion in cellulose derivative matrices. Carbohydr Polym, 98: 1108-16.
  • Margulis K, Magdassi S, Lee HS, Macosko, CW, 2014. Formation of curcumin nanoparticles by flash nanoprecipitation from emulsions. J Colloid Interface Sci, 434: 65-70.
  • Mohanty C, Sahoo SK, 2010 The in vitro stability and in vivo pharmacokinetics of curcumin prepared as an aqueous nanoparticulate formulation. Biomaterials, 31: 6597-611.
  • Rai S, Paliwal R, Gupta PN, Khatri K, Goyal AK, Vaidya B, Vyas, SP, 2008. Solid lipid nanoparticles (SLNs) as a rising tool in drug delivery science: One step up in nanotechnology. Current Nanoscience, 4: 30-44.
  • Saha GB. 2010. Fundamentals of Nuclear Pharmacy. Sixth Edition, Springer Science & Business Media, USA. 83 p.
  • Yadav D, Kumar N, 2014. Nanonization of curcumin by antisolvent precipitation: process development, characterization, freeze drying and stability performance. Int J Pharm, 477: 564-77.
  • Yadav VR, Prasad S, Kannappan R, Ravindran J, Chaturvedi MM, Vaahtera L, Parkkinen J, Aggarwal BB, 2010. Cyclodextrincomplexed curcumin exhibits anti-inflammatory and antiproliferative activities superior to those of curcumin through higher cellular uptake. Biochem Pharmacol, 80: 1021-32.
  • Zolle I 2007. Technetium-99m Pharmaceuticals: PreparationAndQuality Control In Nuclear Medicine. Springer, Berlin. 123-149 p

Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü

Year 2016, Volume: 6 Issue: 3, 27 - 34, 30.09.2016

Abstract

Son zamanlarda, hastalıkların tanı ve tavisini kolaylaştırmak amacıyla, nanoteknoloji ve
moleküler görüntüleme alanları, çok fonksiyonlu nanopartiküllerin oluşturulması ile birleştirilmiştir.
Nanopartiküllerden oluşturulan yeni nesil görüntüleme ajanlarının sahip olduğu çok yönlü ve eşsiz
özelliklerinden faydalanan alanlardan biriside nükleer tıp alanıdır. Yapılan bu çalışmada; kurkumin
yüklü katı lipit nanopartiküllerin (K-KLN) karaciğer ve dalak sintigrafsinde kullanılabilirliliği
araştırıldı. Öncelikle, kurkumin yüklü katı lipit nanopartiküller (K-KLN) mikroemülsiyon ve düşüksıcaklıkda katılaştırma yöntemiyle hazırlandı. Lazer kırınımı (LD) analizi ile partikül boyutu belirlendi.
Termogravimetrik analizi (TGA) ile K-KLN’lerin sıcaklığa karşı stabil olduğu gözlemlendi. Daha sonra
K-KLN’ler Teknesyum-99m (
99mTc) ile radyoaktif olarak işaretlendi. İnce katman kromatograf yöntemi
ile radyoaktif işaretleme etkinliği %95’den daha fazla olarak bulundu.
99mTc-K-KLN’ler intravenöz (i.v.)
olarak tavşana enjekte edildi ve gama kamera ile dinamik görüntüler kaydedildi. Elde edilen dinamik
görüntülerde karaciğer ve dalak belirgin bir şekilde görüntülendi. Sintigrafk bulgulara ek olarak
yapılan ex vivo biyodağılım çalışmaları ve floresan mikroskop çalışmalarında karaciğer ve dalakta
99mTc-K-KLN’lerin varlığı gözlemlendi.  

References

  • Aburahma MH, Badr-Eldin SM, 2014. Compritol 888 ATO: a multifunctional lipid excipient in drug delivery systems and nanopharmaceuticals. Expert Opin Drug Deliv, 11: 1865-83.
  • Aggarwal BB,Harikumar KB, 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol, 41: 40-59.
  • Andreozzi E, Seo JW, Ferrara K, Louie A, 2011, Novel method to label solid lipid nanoparticles with 64cu for positron emission tomography imaging. Bioconjug Chem, 22: 808-18.
  • Anwar M, Asfer M, Prajapati AP, Mohapatra S, Akhter S, Ali A, Ahmad, FJ, 2014. Synthesis and in vitro localization study of curcumin-loaded SPIONs in a micro capillary for simulating a targeted drug delivery system. Int J Pharm, 468: 158-64.
  • Banerjee I, De K, Chattopadhyay S, Bandyopadhyay AK, Misra M, 2014. An easy and effective method for radiolabelling of solid lipid nanoparticles. J Radioanal Nucl Chem, 302:837–843.
  • Gao Y, Li Z, Sun M, Guo C, Yu A, Xi Y, Cui J, Lou H, Zhai G, 2011. Preparation and characterization of intravenously injectable curcumin nanosuspension. Drug Deliv, 18: 131-42.
  • Kakkar V, Mishra AK, Chuttani K, Kaur IP, 2013. Proof of concept studies to confrm the delivery of curcumin loaded solid lipid nanoparticles (C-SLNs) to brain. Int J Pharm, 448: 354-9.
  • Kakkar V, Muppu SK, Chopra K, Kaur IP, 2013. Curcumin loaded solid lipid nanoparticles: an effcient formulation approach for cerebral ischemic reperfusion injury in rats. Eur J Pharm Biopharm, 85: 339-45.
  • Kathe N, Henriksen B, Chauhan H, 2014. Physicochemical characterization techniques for solid lipid nanoparticles: principles and limitations. Drug Dev Ind Pharm, 40: 1565-75.
  • Kumar SS, Mahesh A, Mahadevan S, Mandal AB, 2014. Synthesis and characterization of curcumin loaded polymer/lipid based nanoparticles and evaluation of their antitumor effects on MCF-7 cells. Biochim Biophys Acta, 1840: 1913-22.
  • Li B, Konecke S, Wegiel LA Taylor, LS, Edgar KJ, 2013. Both solubility and chemical stability of curcumin are enhanced by solid dispersion in cellulose derivative matrices. Carbohydr Polym, 98: 1108-16.
  • Margulis K, Magdassi S, Lee HS, Macosko, CW, 2014. Formation of curcumin nanoparticles by flash nanoprecipitation from emulsions. J Colloid Interface Sci, 434: 65-70.
  • Mohanty C, Sahoo SK, 2010 The in vitro stability and in vivo pharmacokinetics of curcumin prepared as an aqueous nanoparticulate formulation. Biomaterials, 31: 6597-611.
  • Rai S, Paliwal R, Gupta PN, Khatri K, Goyal AK, Vaidya B, Vyas, SP, 2008. Solid lipid nanoparticles (SLNs) as a rising tool in drug delivery science: One step up in nanotechnology. Current Nanoscience, 4: 30-44.
  • Saha GB. 2010. Fundamentals of Nuclear Pharmacy. Sixth Edition, Springer Science & Business Media, USA. 83 p.
  • Yadav D, Kumar N, 2014. Nanonization of curcumin by antisolvent precipitation: process development, characterization, freeze drying and stability performance. Int J Pharm, 477: 564-77.
  • Yadav VR, Prasad S, Kannappan R, Ravindran J, Chaturvedi MM, Vaahtera L, Parkkinen J, Aggarwal BB, 2010. Cyclodextrincomplexed curcumin exhibits anti-inflammatory and antiproliferative activities superior to those of curcumin through higher cellular uptake. Biochem Pharmacol, 80: 1021-32.
  • Zolle I 2007. Technetium-99m Pharmaceuticals: PreparationAndQuality Control In Nuclear Medicine. Springer, Berlin. 123-149 p
There are 18 citations in total.

Details

Primary Language Turkish
Journal Section Biyomedikal Mühendisliği / Biomedical Engineering
Authors

Hayrettin Eroğlu

Ayşe Yenilmez This is me

Arif Kursad Ayan This is me

Cemal Gündoğdu This is me

Sare Şipal This is me

Publication Date September 30, 2016
Submission Date April 2, 2016
Acceptance Date May 10, 2016
Published in Issue Year 2016 Volume: 6 Issue: 3

Cite

APA Eroğlu, H., Yenilmez, A., Ayan, A. K., Gündoğdu, C., et al. (2016). Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü. Journal of the Institute of Science and Technology, 6(3), 27-34.
AMA Eroğlu H, Yenilmez A, Ayan AK, Gündoğdu C, Şipal S. Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü. J. Inst. Sci. and Tech. September 2016;6(3):27-34.
Chicago Eroğlu, Hayrettin, Ayşe Yenilmez, Arif Kursad Ayan, Cemal Gündoğdu, and Sare Şipal. “Tc-99m Ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü”. Journal of the Institute of Science and Technology 6, no. 3 (September 2016): 27-34.
EndNote Eroğlu H, Yenilmez A, Ayan AK, Gündoğdu C, Şipal S (September 1, 2016) Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü. Journal of the Institute of Science and Technology 6 3 27–34.
IEEE H. Eroğlu, A. Yenilmez, A. K. Ayan, C. Gündoğdu, and S. Şipal, “Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü”, J. Inst. Sci. and Tech., vol. 6, no. 3, pp. 27–34, 2016.
ISNAD Eroğlu, Hayrettin et al. “Tc-99m Ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü”. Journal of the Institute of Science and Technology 6/3 (September 2016), 27-34.
JAMA Eroğlu H, Yenilmez A, Ayan AK, Gündoğdu C, Şipal S. Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü. J. Inst. Sci. and Tech. 2016;6:27–34.
MLA Eroğlu, Hayrettin et al. “Tc-99m Ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü”. Journal of the Institute of Science and Technology, vol. 6, no. 3, 2016, pp. 27-34.
Vancouver Eroğlu H, Yenilmez A, Ayan AK, Gündoğdu C, Şipal S. Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü. J. Inst. Sci. and Tech. 2016;6(3):27-34.