Hen’s Egg Test For Micronucleus Induction (HET-MN)

Year 2016, Volume: 6 Issue: 4, 17 - 28, 31.12.2016

Haluk Özparlak , Bülent Çelik

Abstract

One of widely used methods in genotoxicity tests is micronucleus technique. Hen’s
Egg Test for Micronucleus Induction (HET-MN) is a member of relatively new of micronucleus tests.
HET-MN is an extremely simple, inexpensive and rapid genotoxicity assay which is positioned between
pure in vitro and in vivo analysis, strictly in line with animal protection regulations and ethical aspects.
Furthermore the high metabolic competency provided by this model enables metabolic activation of
xenobiotics including mutagens and promutagens. In this article, recent publications about the kinds of
micronucleus tests and then the application of HET-MN have been compiled  

Keywords

Chicken embryo, erythrocyte, genotoxicity, micronucleus

Tavuk Yumurtası Mikronükleus Testi

Abstract

Genotoksisite testleri içinde çok yaygın kullanılan yöntemlerden biri mikronükleus tekniğidir.
Mikronükleus testleri içerisinde ise diğerlerine kıyasla daha yeni bir teknik Tavuk Yumurtası Mikronükleus
Testi (Hen’s Egg Test for Micronucleus Induction, HET-MN)’dir. HET-MN hayvan hakları ve etik
açıdan kesinlikle uygun, in vitro ve in vivo deneyler arasına yerleştirebileceğimiz son derece basit, ucuz
ve hızlı bir genotoksisite testidir. Ayrıca bu modelin sağladığı yüksek metabolik yetkinlik mutajen ve
promutajenleri içeren ksenobiyotiklerin metabolik aktivasyonuna da imkân tanımaktadır. Bu makalede
önce mikronükleus testlerinin çeşitleri, ardından HET-MN’nin uygulamasıyla ilgili güncel yayınlar
derlenmiştir  

Keywords

Eritrosit, genotoksisite, mikronükleus, tavuk embriyosu

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