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The Effects of Iron (Fe+3) on The Expression Levels of Heat Stress Protein Genes in Rat Liver (Rattus norvegicus) Tissue

Year 2018, Volume: 8 Issue: 1, 255 - 261, 31.03.2018
https://doi.org/10.21597/jist.407883

Abstract

Iron, one of the most common metals in the world, is important for organisms. It is known that at

high concentrations, it damages especially organs such as liver, pancreas, heart. In our study, the effect of iron

ion on the expression of the 70 kDa HSP gene family which small stress proteins was investigated in Rattus

norvegicus. In this study, iron ion Fe3 + (0,87ppm, 3ppm, 30ppm, 300ppm) was given to 5 different application

groups at different concentrations. At the end of this application period, a cDNA library was formed from the liver

tissues taken from the living body. Using these libraries, changes in expression levels occurring in the HSP70

(Hspa1a, Hspa4, Hspa5) HSP90 (Hsp90aa1) genes were determined by Real-Time PCR method.

References

  • Ait-Aissa S, Porcher J, Arrigo A, Lambre C, 2000. Activation of the hsp70 promoter by environmental inorganic and organic chemicals: relationships with cytotoxicity and lipophilicity. Toxicology, 145:147–157.
  • Anonym ,2012. http://www.ncbi.nlm.nih.gov/. (Erişim tarihi: 18 Eylül, 2012).
  • Anonym, 2012. http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi/.(Erişim tarihi: 18 Eylül, 2012).
  • Anonym, 2012. http://www.ncbi.nlm.nih.gov/BLAST/ . (Erişim tarihi: 18 Eylül, 2012).
  • Boone AN, Vijayan MM, 2002. Constitutive heat shock protein 70 (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure. Comp Biochem Physiol C Toxicol Pharmacol , 132(2):223-33.
  • Bowen ID, Mullarkey K, Morgan SM, 1996. Programmed cell death in the salivary gland of the blow fly Calliphora vomitoria, Microsc. Res. Techniq, 34:202–217.
  • Deane EE, Zhou L, Woo NY, 2006. Cortisol can be pro- or anti-apoptotic in sea bream cells: potential role of HSP70 induction for cytoprotection. Mol Cell Endocrinol, 259:57–64.
  • Eddie E, Deane EE , Woo NY, Norman YS, 2006. Impact of heavy metals and organochlorines on hsp70 and hsc70 gene expression in black sea bream fibroblasts. Aquatic Toxicology Amsterdam, Netherlands, 79(1):9-15.
  • Encomio VG , Stickler SM, Allen SK And Chu FL, 2005. Performance of “natural dermo-resistant” oyster stocks – survival, disease, growth, condition and energy reserves. Journal of Shellfish Research, 24: 143- 155.
  • Heydari AR, Wu B, Takahashi R, Strong R, Richardson A, 1993. Expression of heat shock protein 70 is altered by age and diet at the level of transcription. Mol Cell Biol, 13: 2909–2918.
  • Jolly C, Morimoto RI, 2000. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. Journal of the National Cancer Institute, 92: 1564–1572.
  • Keller JM, Escara Wilke F, Keller ET, 2008. Heat stress-induced heat shock protein 70 expression is dependent on ERK activation in zebrafish (Danio rerio) cells. Comparative Biochemistry and Physiology, 150: 307-314
  • Köhler HR, Triebskorn R, Stöcker W, Kloetzel PM, Alberti G, 1992. The 70 kD heat shock protein (hsp 70) in soil invertebrates: a possible tool for monitoring environmental toxicants. “Arch Environ Contam Toxicol, 22: 334–338.
  • Krone PH, Lele Z, 1997. Hsp47 and Hsp70 gene expression is differentially regulated in a stress-and tissue-specific manner in zebrafish embryos. Cell. Mol. Life Sci Locke M, Tanguay RM ,21(2):123-33.
  • Lindquist S, Henikoff S, and Meselson M, 1975. Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc. Natl. Acad. Sci, 72: 1117-1121.
  • Livak KJ, Schmittgen TD., 2001. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Elsevier Science (USA), 25(4):402-8.
  • Marius L, Robert M,1996. Tanguay Increased HSF activation in muscles with a high constitutive HSP 70 expression. Cell Stress Chaperones,1: 189–196
  • Mathew A, Morimoto RI, 1998. Role of the heat-shock response in the life and death of proteins. Ann NY Acad Sci, 851:99-111.
  • Mesa MG, Weiland LK, Wagner P, 2002. Effects of acute thermal stress on the survival predator avoidance, and physiology of juvenile fall Chinook salmon. Northwest Sci, 76: 118–128.
  • Mezquita B, Mezquita J, Durfort M, 2001. Constitutive and heat-shock induced expression of Hsp70 mRNA during chicken testicular development and regression. J. Cell. Biol, 82 (3): 480-490.
  • Murtha JM, Keller ET, 2003. Characterization of the heat shock response in mature zebrafish (Danio rerio). Exp Gerontol, 38:683–691.
  • Palmisano AN, Winton JR, Dickhoff W.W, 2000. Tissue specific induction of Hsp90 mRNA and plasma cortisol response in chinook salmon following heat shock, seawater challenge, and handling challenge. Marine Biotechnology, 2: 329-338.
  • Pardu ML, Ballinger DG, Hogan NC, 1992. The heat shock response. Cells coping with transient stress. Annals of the New York 125–138 p.
  • Sivakumar R, Jayprakash M , Natesan M, 2013. Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India. Ecotoxicol Environ Saf, 98:8-18.
  • Sreedhar AS, Csermely P, 2004. Heat Shock Proteins In The Regulation Of Apoptosis: New Strategies In Tumor Therapy. A Comprehensive Review.Pharmacology And Therapeutics, 101: 227-257.
  • Yan LJ, Christians ES, Liu L, Xiao X , Sohal R.S , Benjamin IJ , 2002. Mouse Heat Shock Transcription Factor 1 Deficiency Alters Cardiac Redox Homeostasis And Increases Mitochondrial Oxidative Damage. The Journal of Embo, 21: 5164-5172.
  • Yahav S, 2009. Alleviating heat stress in domestic fowl: different strategies. World’s Poult Sci J, 65:719–732.
  • Zuhal U, 2009. Pseudomonas Sp. Suslarinda Cold Shock Protein Izolasyonu Ve Sds–Page Analizi. Çukurova Üniversitesi Fen Bilimleri Enititüsü, Yüksek Lisans Tezi, 80s.

Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi

Year 2018, Volume: 8 Issue: 1, 255 - 261, 31.03.2018
https://doi.org/10.21597/jist.407883

Abstract

Dünyada en fazla bulunan metallerden birisi olan demir, organizmalar için önemlidir. Yüksek
konsantrasyonlarda özellikle karaciğer, pankreas, kalp gibi organlarda hasar oluşturduğu bilinmektedir.
Çalışmamızda Rattus norvegicus’da demir iyonunun küçük stress proteinleri olan 70 kDa HSP gen ailesinin
ekspresyonu üzerine olan etkisi araştırıldı. Bu çalışma kapsamında 5 farklı uygulama grubuna farklı
konsantrasyonlarda demir iyonu Fe3+ (0,87ppm, 3ppm, 30ppm, 300 ppm) verildi. Bu uygulama süreci sonunda
canlıdan alınan karaciğer dokularından cDNA kütüpanesi oluşturuldu. Yapılan bu kütüpaneler kullanılarak HSP70
(Hspa1a, Hspa4, Hspa5) HSP90 (Hsp90aa1) genlerinde meydana gelen ekspresyon seviyelerindeki değişimler
Real-Time PCR metodu ile tespit edildi.

References

  • Ait-Aissa S, Porcher J, Arrigo A, Lambre C, 2000. Activation of the hsp70 promoter by environmental inorganic and organic chemicals: relationships with cytotoxicity and lipophilicity. Toxicology, 145:147–157.
  • Anonym ,2012. http://www.ncbi.nlm.nih.gov/. (Erişim tarihi: 18 Eylül, 2012).
  • Anonym, 2012. http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi/.(Erişim tarihi: 18 Eylül, 2012).
  • Anonym, 2012. http://www.ncbi.nlm.nih.gov/BLAST/ . (Erişim tarihi: 18 Eylül, 2012).
  • Boone AN, Vijayan MM, 2002. Constitutive heat shock protein 70 (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure. Comp Biochem Physiol C Toxicol Pharmacol , 132(2):223-33.
  • Bowen ID, Mullarkey K, Morgan SM, 1996. Programmed cell death in the salivary gland of the blow fly Calliphora vomitoria, Microsc. Res. Techniq, 34:202–217.
  • Deane EE, Zhou L, Woo NY, 2006. Cortisol can be pro- or anti-apoptotic in sea bream cells: potential role of HSP70 induction for cytoprotection. Mol Cell Endocrinol, 259:57–64.
  • Eddie E, Deane EE , Woo NY, Norman YS, 2006. Impact of heavy metals and organochlorines on hsp70 and hsc70 gene expression in black sea bream fibroblasts. Aquatic Toxicology Amsterdam, Netherlands, 79(1):9-15.
  • Encomio VG , Stickler SM, Allen SK And Chu FL, 2005. Performance of “natural dermo-resistant” oyster stocks – survival, disease, growth, condition and energy reserves. Journal of Shellfish Research, 24: 143- 155.
  • Heydari AR, Wu B, Takahashi R, Strong R, Richardson A, 1993. Expression of heat shock protein 70 is altered by age and diet at the level of transcription. Mol Cell Biol, 13: 2909–2918.
  • Jolly C, Morimoto RI, 2000. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. Journal of the National Cancer Institute, 92: 1564–1572.
  • Keller JM, Escara Wilke F, Keller ET, 2008. Heat stress-induced heat shock protein 70 expression is dependent on ERK activation in zebrafish (Danio rerio) cells. Comparative Biochemistry and Physiology, 150: 307-314
  • Köhler HR, Triebskorn R, Stöcker W, Kloetzel PM, Alberti G, 1992. The 70 kD heat shock protein (hsp 70) in soil invertebrates: a possible tool for monitoring environmental toxicants. “Arch Environ Contam Toxicol, 22: 334–338.
  • Krone PH, Lele Z, 1997. Hsp47 and Hsp70 gene expression is differentially regulated in a stress-and tissue-specific manner in zebrafish embryos. Cell. Mol. Life Sci Locke M, Tanguay RM ,21(2):123-33.
  • Lindquist S, Henikoff S, and Meselson M, 1975. Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc. Natl. Acad. Sci, 72: 1117-1121.
  • Livak KJ, Schmittgen TD., 2001. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Elsevier Science (USA), 25(4):402-8.
  • Marius L, Robert M,1996. Tanguay Increased HSF activation in muscles with a high constitutive HSP 70 expression. Cell Stress Chaperones,1: 189–196
  • Mathew A, Morimoto RI, 1998. Role of the heat-shock response in the life and death of proteins. Ann NY Acad Sci, 851:99-111.
  • Mesa MG, Weiland LK, Wagner P, 2002. Effects of acute thermal stress on the survival predator avoidance, and physiology of juvenile fall Chinook salmon. Northwest Sci, 76: 118–128.
  • Mezquita B, Mezquita J, Durfort M, 2001. Constitutive and heat-shock induced expression of Hsp70 mRNA during chicken testicular development and regression. J. Cell. Biol, 82 (3): 480-490.
  • Murtha JM, Keller ET, 2003. Characterization of the heat shock response in mature zebrafish (Danio rerio). Exp Gerontol, 38:683–691.
  • Palmisano AN, Winton JR, Dickhoff W.W, 2000. Tissue specific induction of Hsp90 mRNA and plasma cortisol response in chinook salmon following heat shock, seawater challenge, and handling challenge. Marine Biotechnology, 2: 329-338.
  • Pardu ML, Ballinger DG, Hogan NC, 1992. The heat shock response. Cells coping with transient stress. Annals of the New York 125–138 p.
  • Sivakumar R, Jayprakash M , Natesan M, 2013. Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India. Ecotoxicol Environ Saf, 98:8-18.
  • Sreedhar AS, Csermely P, 2004. Heat Shock Proteins In The Regulation Of Apoptosis: New Strategies In Tumor Therapy. A Comprehensive Review.Pharmacology And Therapeutics, 101: 227-257.
  • Yan LJ, Christians ES, Liu L, Xiao X , Sohal R.S , Benjamin IJ , 2002. Mouse Heat Shock Transcription Factor 1 Deficiency Alters Cardiac Redox Homeostasis And Increases Mitochondrial Oxidative Damage. The Journal of Embo, 21: 5164-5172.
  • Yahav S, 2009. Alleviating heat stress in domestic fowl: different strategies. World’s Poult Sci J, 65:719–732.
  • Zuhal U, 2009. Pseudomonas Sp. Suslarinda Cold Shock Protein Izolasyonu Ve Sds–Page Analizi. Çukurova Üniversitesi Fen Bilimleri Enititüsü, Yüksek Lisans Tezi, 80s.
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Authors

Atena Ghosıgharehaghajı This is me 0000-0002-8856-8287

Hamid Ceylan This is me 0000-0003-3781-4406

Orhan Erdoğan 0000-0001-8908-7293

Publication Date March 31, 2018
Submission Date September 5, 2017
Acceptance Date October 31, 2017
Published in Issue Year 2018 Volume: 8 Issue: 1

Cite

APA Ghosıgharehaghajı, A., Ceylan, H., & Erdoğan, O. (2018). Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi. Journal of the Institute of Science and Technology, 8(1), 255-261. https://doi.org/10.21597/jist.407883
AMA Ghosıgharehaghajı A, Ceylan H, Erdoğan O. Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi. J. Inst. Sci. and Tech. March 2018;8(1):255-261. doi:10.21597/jist.407883
Chicago Ghosıgharehaghajı, Atena, Hamid Ceylan, and Orhan Erdoğan. “Demir İyonunun (Fe3+) Sıçan (Rattus Norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi”. Journal of the Institute of Science and Technology 8, no. 1 (March 2018): 255-61. https://doi.org/10.21597/jist.407883.
EndNote Ghosıgharehaghajı A, Ceylan H, Erdoğan O (March 1, 2018) Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi. Journal of the Institute of Science and Technology 8 1 255–261.
IEEE A. Ghosıgharehaghajı, H. Ceylan, and O. Erdoğan, “Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi”, J. Inst. Sci. and Tech., vol. 8, no. 1, pp. 255–261, 2018, doi: 10.21597/jist.407883.
ISNAD Ghosıgharehaghajı, Atena et al. “Demir İyonunun (Fe3+) Sıçan (Rattus Norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi”. Journal of the Institute of Science and Technology 8/1 (March 2018), 255-261. https://doi.org/10.21597/jist.407883.
JAMA Ghosıgharehaghajı A, Ceylan H, Erdoğan O. Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi. J. Inst. Sci. and Tech. 2018;8:255–261.
MLA Ghosıgharehaghajı, Atena et al. “Demir İyonunun (Fe3+) Sıçan (Rattus Norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi”. Journal of the Institute of Science and Technology, vol. 8, no. 1, 2018, pp. 255-61, doi:10.21597/jist.407883.
Vancouver Ghosıgharehaghajı A, Ceylan H, Erdoğan O. Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi. J. Inst. Sci. and Tech. 2018;8(1):255-61.