In Vitro Shoot Regeneration of Limnophila aromatica (Lamk.) Merr. from Nodal and Internodal Explants

Year 2018, Volume: 8 Issue: 3, 77 - 84, 30.09.2018

Muhammet Doğan

Abstract

Limnophila aromatica (Lamk.) Merr. is an important medicinal and aromatic plant widely used in

the traditional medicine. In this study, nodal and internodal explants of L. aromatica (Lamk.) Merr. were cultured

for multiple and rapid production on Murashige and Skoog (MS) medium containing combinations of 0.10 mg

L-1 Gibberellic acid (GA3) and 0.05, 0.10, 0.20, 0.40, 0.80 and 1.60 mg L-1 Thidiazuron (TDZ) for eight weeks.

In general, high shoot regeneration percentages were obtained for both types of explants. In nodal explants, the

maximum number of shoots per explant (21.44) and the highest shoot length (1.68 cm) were determined in MS

medium containing 0.10 mg L-1 GA3 + 0.40 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.10 mg L-1 TDZ, respectively.

In internodal explants, the maximum number of shoots per explant (17.46) and the highest shoot length (1.60 cm)

were obtained in MS medium supplemented with 0.10 mg L-1 GA3 + 0.20 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.05

mg L-1 TDZ, respectively. With the effect of plant growth regulators, the nodal explants gave more regenerated

shoots than the internodal explants. Regenerated shoots were successfully rooted on MS medium containing 0.25

mg L-1 indole butyric acid (IBA). Successful adaptation of the rooted shoots to the aquarium environment has been

achieved.

Keywords

Nodal explant, propagation, in vitro, Limnophila aromatica, TDZ

Limnophila aromatica (Lamk.) Merr.’nın Boğum ve Boğum Arası Eksplantlarından In Vitro Sürgün Rejenerasyonu

Abstract

Limnophila aromatica (Lamk.) Merr. geneneksel tıpta yaygın şekilde kullanılan önemli tıbbi ve aromatik

bir bitkidir. Bu çalışmada, L. aromatica’nın çoklu ve hızlı üretimi için boğum ve boğum arası eksplantları 0.10 mg

L-1 Gibberellik asit (GA3) ve 0.05, 0.10, 0.20, 0.40, 0.80 ve 1.60 mg L-1 Tidiazuron (TDZ) kombinasyonlarını içeren

Murashige ve Skoog (MS) besin ortamında sekiz hafta boyunca kültüre alınmıştır. Genel olarak her iki tip eksplant

için de yüksek sürgün rejenerasyon yüzdeleri elde edilmiştir. Boğum eksplantlarında eksplant başına sürgün sayısı

(21.44 adet) ve en uzun sürgünler (1.68 cm) sırasıyla 0.10 mg L-1 GA3 + 0.40 mg L-1 TDZ ve 0.10 mg L-1 GA3 +

0.10 mg L-1 TDZ içeren MS ortamında tespit edilmiştir. Boğum arası eksplantlarında, maksimum eksplant başına

sürgün sayısı (17.46 adet) ve en uzun sürgünler (1.60 cm) sırasıyla 0.10 mg L-1 GA3 + 0.20 mg L-1 TDZ ve 0.10

mg L-1 GA3 + 0.05 mg L-1 TDZ eklenmiş MS ortamında elde edilmiştir. Bitki büyüme düzenleyicilerinin etkisi ile

boğum eksplantı, boğumarası eksplantından daha fazla rejenere sürgün vermiştir. Rejenere sürgünler 0.25 mg L-1

indol butirik asit (IBA) içeren MS ortamında başarılı bir şekilde köklendirilmiştir. Köklenen sürgünlerin akvaryum

ortamına alıştırılması başarıyla sağlanmıştır.

Keywords

Boğum eksplant, çoğaltım, in vitro, Limnophila aromatica, TDZ

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