CaO and Graphene Oxide Enhances Drought Stress from Callus Tissues of Medicago Sativa L. Cultivars

Year 2022, Volume: 12 Issue: 4, 2450 - 2458, 01.12.2022

Büşra Yazıcılar , İsmail Bezirganoglu , Yen Ling Chang Muthukumar Nadar

https://doi.org/10.21597/jist.1125699

Cited By: 1

Abstract

Drought stress can be described as multidimensional stress factors affecting plants’ growth, development, and productivity. In order to reduce the adverse impact of drought stress, a plethora of attempts have been employed. Among those attempts, nano-engineered particles have gained a remarkable attention. Of the relevant particles, calcium oxide (CaO) and graphene oxide (GO) have been well-documented to positively regulate and mediate the plant growth system through shifting physiological biochemical and molecular aspects of the plant. The solo impacts of the nanoparticles are well-known but their interactions were not assayed for Medicago sativa L. cultivars. For that reason, the present study investigates the impact of CaO NPs and GO on the response and regulation of the defensive mechanism in alfalfa (Medicago sativa L.) callus in drought stress-suffered cultivars.
The activation of CaO-GO can be induced with mannitol in the callus of alfalfa cultivars. Dry and fresh weight values were determined in callus samples. There were significant differences between cultivars and concentration. In terms of MDA, H2O2, proline content, it was observed that the Ca2+ NPs application was important, and it showed a strong link with the resistance degree of cultivars. Erzurum cultivar was observed for better proline content with 1.5 ppm GO. MDA activities demonstrated an increasing trend concerning concentrations of mannitol and nanoparticles. The MDA highest activity was observed with 1/2 ppm CaO+0.5/1.5 ppm GO (0.1849 mg/g FW) in the Erzurum. However, the Erzurum cultivar responded with better H2O2 content with 100 mM mannitol +0.5 ppm (0.1017 mg/g FW). This result has presented, under in vitro conditions, that the supplementation of CaO and GO can importantly reduce the negative impacts of drought stress on alfalfa callus; additionally, it has been seen that the dosages of nanoparticle and mannitol are also important.

Keywords

Alfalfa, nanoparticules, mannitol, in vitro culture

Project Number

2021/024

References

• Abdelsalam NR, Balbaa MG, Osman HT, Ghareeb RY, Desoky ESM, Elshehawi AM, 2022. Inheritance of resistance against northern leaf blight of maize using conventional breeding methods. Saudi J. Biol. Sci. 29: 1747–1759.
• Arora S, Sharma P, Kumar S, Nayan R, Khanna PK, Zaidi MGH, 2012. Gold-nanoparticle induced
• enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul 66: 303–310.
• Ayyaz A, Miao Y, Hannan F, Islam F, Zhang K, Xu J, 2021. Drought tolerance in Brassica napusis accompanied with enhanced antioxidative protection, photosynthetic and hormonal regulation at seedling stage. Physiologia Plantarum, 172: 1129–1144.
• Bano H, Athar HR, Zafar Z, Kalaji HM, Ashraf M, 2021. Linkingchanges in chlorophylla Fluorescence with drought stress susceptibil-ity in mung bean [Vigna radiata (L.) Wilczek]. Physiologia Plantarum,172: 1240–1250.
• Bothwell JHF, Ng, CKY, 2005. The evolution of Ca21 signalling in photosynthetic eukaryotes. New Phytol. 166, 21–38.
• Castroluna A, Ruiz OM, Quiroga AM, 2014. Effects of salinity and drought stress on germination, biomass and growt in three varieties of Medicago sativa L. Avances Invest. Agropes. 18: 39-50.
• Cheong YH, Pandey GK, Grant JJ, Batistic O, Li L, Kim BG, Lee SC, Kudla J, Luan S, 2007. Two calcineurin B-like calcium sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis. Plant J 52: 223-239.
• Chong Y, Ma Y, Shen H, Tu X, Zhou X, Xu J, 2014. The in vitro and in vivo toxicity of graphene quantum dots. Biomaterials 35: 5041–5048. doi: 10.1016/j.biomaterials.2014.03.021
• Elrys AS, Abdo AIE, Abdel-Hamed EMW, Desoky ES, 2020. Integrative application of licorice root extract or lipoic acid with fulvic acid improves wheat production and defenses under salt stress conditions. Ecotoxicology and Environmental Safety 190: 110144.
• Erdal S, 2012. Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress. Plant Physiol Biochem 57: 1–7.
• Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M, Skali Senhaji N, 2007. Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures. Acta Physiol. Plant. 29(2): 95–102.
• Jiang QD, Roche T, Monaco S, Durham, 2006. Gas exchange, chlorophyll fluorescence parameters, and carbon isotope discrimination of fourteen barley genetic lines in response to salinity.
• Kapilan R, Vaziri M, Zwiazek JJ, 2018. Regulation of aquaporins in plants under stress. Biol. Res.51:4. Kokura S, Handa O, Takagi T, Ishikawa T, Naito Y, Yoshikawa T, 2010. Silver nanoparticles as a safe preservative for use in cosmetics. Nanomed Nanotechnol Biol Med 6(4): 70–74.
• Kong X, Lv, Wiang S, Dan Z, Cai G, Pan J, Li D, 2013. Genome-wide identification and expression analysis of calcium-dependent protein kinase in maize. BMC Genom. 14: 433.
• Le VD, 2019. Nanoparticles for the improved crop production. In: Panpatte DG, Jhala YK (eds) Nanotechnology for agriculture: crop production & protection. Springer Singapore, Singapore, pp 85–106.
• Murashige T, Skoog FA, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473–497.
• Nazari F, Jafarirad S, Movafeghi A, Kosari-Nasab M, Kazemi EM, 2020. Toxicity of microwave- synthesized silver reduced graphene oxide nanocomposites to the microalga Chlorella vulgaris: comparison with the hydrothermal method synthesized counterparts. J. Environ. Sci. Health A. 6, 639–649.
• Regier N, Cosio C, von Moos N, Slaveykova VI, 2015. Effects of copper- oxide nanoparticles, dissolved copper and ultraviolet radiation on copper bioaccumulation, photosynthesis and oxidative stress in the aquatic macrophyte Elodea nuttallii. Chemosphere 128: 56–61.
• Rodriguez RJ, Redman RS, 2005. Balancing the generation and elimination of reactive oxygen species. Proc Natl Acad Sci USA 102, 3175-3176.
• Salih BA, Azeez KO, 2019. Antidiabetic action of alfalfa (Medicago sativa) leaves powder on type II diabetic patients. Polytechnic Journal. (1): 23–25.
• Sanders D, Pelloux J, Brownlee C, Harper JF, 2002. Calcium at the crossroads of signaling. Plant Cell 14 (1): 401–417.
• Shao HB, Song WY, Chu LY, 2008. Advances of calcium signals involved in plant anti-drought. Comptes Rendus Biol. 331: 587–596.
• Shi H, Wang Y, Cheng Z, Ye T, Chan Z, 2021a. Analysis of natural variation in bermudagrass (Cynodondactylon) reveals physiological responses under lying drought tolerance. PLoSONE, 7,E53422. doi: 10.1371/journal.pone.0053422
• Singh M, Singh S, Prasad S, Gambhir IS, 2008. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Dig J Nanomater Biostruct 3(3): 115–122.
• Sitohy MZ, Desoky EM, Osman A, Rady MM, 2020. Pumpkin seed protein hydrolysate treatment alleviates salt stress effects on Phaseolus vulgaris by elevating antioxidant capacity and recovering ion homeostasis. Sci. Hortic. 271: 109495.
• Tiede K, Boxall AB, Tear SP, Lewis J, David H, Hassello¨v M, 2008. Detection and characterization of engineered nanoparticles in food and the environment. Food Addit Contam 25(7): 795–821.
• Usman M, 2020. Nanotechnology in agriculture: current status, challenges and future opportunities. Sci Total Environ 721,137778.
• Uzun S, 1997. Sıcaklık ve ışığın bitki büyüme, gelişme ve verimine etkisi (I. Büyüme). OMÜ Zir. Fak. Dergisi, 12(1): 147-156.
• Velikova V, Yordanov I, Edreva A, 2000. Oxidative stress and some antioxidantsystems in acid rain- treated bean plants protective role of exogenous polyamines. Plant Sci 151: 59–66.
• Wu JW, Geilfus CM, Pitann B, Muhling KH. 2016. Silicon-enhanced oxalate exudation contributes to alleviation of cadmium toxicity in wheat. Environmental and Experimental Botany 131: 10– 18.
• Yazıcılar B, Böke F, Alaylı A, Nadaroglu H, Gedikli S, Bezirganoglu I, 2020. In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long term salt stress. Plant Cell Reports (2021) 40: 29–42.
• Zhao CY, Escalante LN, Chen H, Benatti TR, Qu JX, Chellapilla S, Waterhouse RM, Wheeler D, Andersson MN, Bao RY, 2015. A massive expansion of effector genes underlies gall- formation in the wheat pest Mayetiola destructor. Current Biology 25: 613– 620.
• Xiong R, Liu S, Considine MJ, Siddique KHM, Lam HM, Chen Y, 2020. Root system architecture, physiological and transcriptionaltraits of soybean (Glycine max L.) in response to water deficit: a review. Physiologia Plantarum, 172: 405–418.