Türkiye’nin Bingöl ili Bal Arı Kolonilerinde Deforme kanat virüsü ve Kronik arı felci virüsü’nün Prevalansı

Year 2023, Volume: 13 Issue: 1, 44 - 53, 01.03.2023

Adnan Balkaya Abdullah Güller , Mustafa Usta , Zeynelabidin Kurt

https://doi.org/10.21597/jist.1059418

Abstract

Bal arılarını infekteleyen virüsler dünyanın her yerinde bal arılarının her gelişim evresinde
infeksiyona neden olan ve ekonomik zarara yol açan hastalıklardır. Bu araştırmada dünya
çapında yaygın olan iki bal arısı virüsünün (deforme kanat virüsü [DWV] ve kronik arı paraliz
virüsü [CBPV]) varlığı ve yaygınlığını belirlemek için bir survey çalışması gerçekleştirilmiştir.
Viral RNA’yı belirlemek amacıyla Bingöl ili sınırlarındaki 9 farklı lokaliteden toplam 128
arılık ziyaret edilmiştir. Toplanan 384 bal arısı örneği her virüse özgü genom spesifik primerler
kullanılarak moleküler olarak (revers-transkriptaz polimeraz zincir reaksiyonu, RT-PCR)
testlenmiştir. 128 arılığın 28’i (%21.87) DWV için pozitif reaksiyon vermiştir fakat hiçbir
arılıkta CBPV patojeni tespit edilmemiştir. Pozitif örneklerden rastgele seçilen birinin
klonlanarak ortaya çıkarılan 702 bazlık nükleotit dizisi gen bankasına MZ357973 ulaşım
numarasıyla kaydedilmiştir. NCBI veri tabanındaki BLASTn analizine göre, Bingöl ilinde
belirlenen DWV etmeninin nükleotit dizisi aynı virüsün diğer izolatlarıyla kıyaslandığında
%77.82-98.45 arasında değişen nükleotit benzerliği göstermiştir. Ayrıca, farklı orijinlerden
izolatların kullanıldığı filogenetik ağaca dayanarak bizim izolatımız Türkiye’den ErzincanDWV izolatıyla yakın filogenetik ilişki içerisinde olduğu tespit edilmiştir. Literatür
taramalarına göre, bu çalışma Bingöl ilinde DWV ve CBPV yaygınlığını ve varlığını DNA
temelli yöntemlerle ortaya koyan ilk kayıt niteliğindedir

Keywords

DWV, CBPV, Yayılım, Türkiye, Apis mellifera

Supporting Institution

bulunmamaktadır

Project Number

BAP-ZF.2020.00-004

Thanks

Araştırmada katkısı geçen herkese teşekkür ederim. Ayrıca yayın sürecinde emeği geçen sayın dergi editörü ve sekreteryasına da teşekkürlerimi bildiririm.

Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey

Abstract

Viruses infecting honey bees are diseases that cause infection and economic damage in every
developmental stage of honey bees all over the world. In this study, a survey was conducted to
determine the presence and prevalence of two worldwide common honeybee viruses (deformed
wing virus [DWV] and chronic bee paralysis virus [CBPV]). 128 apiaries from 9 different
localities in the province of Bingöl were visited to determine viral RNA. Collected 384 honey
bee samples were tested molecularly (reverse-transcriptase polymerase chain reaction, RTPCR) using genome-specific primers specific to each virus. In molecular tests, 28 of 128
apiaries (21.87%) gave a positive reaction for DWV, but no CBPV pathogen was detected in
any apiary. The 711 bp nucleotide sequence revealed by cloning one of the randomly selected
positive samples was registered in the gene bank with the accession number MZ357973.
According to the BLASTn analysis in the NCBI database, the nucleotide sequence of the DWV
agent determined in Bingöl Province showed nucleotide similarity between 77.82-98.45%
when compared to other isolates of the same virus. In addition, our isolate was found to be in a
close phylogenetic relationship with the Erzincan-DWV isolate from Turkey, based on the
phylogenetic tree using isolates from different origins. According to our literature screening,
this study is the first record that reveals the prevalence and the presence of DWV and CBPV in
Bingöl Province with DNA-based approaches.

Keywords

DWV, CBPV, Turkey, Apis mellifera, Prevelence

Project Number

BAP-ZF.2020.00-004

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