Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae)

Year 2023, Volume: 13 Issue: 3, 1613 - 1624, 01.09.2023

İrmak Polat , Damla Amutkan Mutlu , Zekiye Suludere

https://doi.org/10.21597/jist.1251065

Abstract

There are many morphological and histological studies in the world to investigate the structure of the external and internal organs of insects. These studies not only serve to illuminate the biology of insect species, but also to reveal taxonomic relationships. For this purpose, the detailed structure of the testis and accessory glands that are the main organs of the male reproductive system of Bolua turkiyae Ünal, 1999 (Orthoptera: Tettigoniidae) was searched with a light microscope, scanning electron microscope, and transmission electron microscope techniques in 2019-2020 in Gazi University, Prof. Dr. Zekiye Suludere Electron Microscope Center in this study. B. turkiyae is an endemic species distributed around Bolu province in Turkey. The reproductive system of male B. turkiyae is comprised of testis, vas deferens, and accessory glands. When the cross-sections of the oval and yellow testis were examined, it was observed that they contain many follicles. There are cysts in these follicles that have the developmental stages of the sperm. From the distal ends of the follicles to the proximal ends, the germarium, growth zone, maturation zone, and transformation zone are located respectively. In these stages, spermatogonium, spermatocyte, spermatids, and finally spermatozoa are formed. Accessory glands are hollow tubular structures of two different sizes and diameters. Single-layered cubic or pyramidal cells surround the lumen in both groups of accessory glands. It is hoped that these new data will contribute to our understanding of the structure of the male reproductive system of B. turkiyae.

Keywords

Endemic species, Insect, Electron microscopy, Morphology, Reproductive system

Supporting Institution

Çankırı Karatekin Üniversitesi BAP

Project Number

FF080120B02

Thanks

We express our thanks to Çankırı Karatekin University, BAP for supporting our study with a project number FF080120B02

Bolua turkiyae (Orthoptera, Tettigoniidae)’nin Testis ve Yardımcı Bezlerinin Morfoloji, Histoloji ve Sitolojisi

Abstract

Böceklerin dış ve iç organlarının yapısını araştırmak için dünyada birçok morfolojik ve histolojik çalışma bulunmaktadır. Bu çalışmalar sadece böcek türlerinin biyolojisini aydınlatmaya değil, aynı zamanda taksonomik ilişkileri ortaya çıkarmaya da hizmet etmektedir. Bu amaçla, bu çalışmada Bolua turkiyae Ünal, 1999 (Orthoptera: Tettigoniidae)'nin erkek üreme sisteminin ana organları olan testis ve yardımcı bezlerin detaylı yapısı ışık mikroskobu, taramalı elektron mikroskobu ve transmisyon elektron mikroskobu teknikleri ile 2019-2020 yıllarında Gazi Üniversitesi, Prof. Dr. Zekiye Suludere Elektron Mikroskop Merkezinde araştırılmıştır. B. turkiyae Türkiye'de Bolu İli çevresinde yayılış gösteren endemik bir türdür. Erkek B. turkiyae üreme sistemi testis, vas deferens ve yardımcı bezlerden oluşmaktadır. Oval ve sarı testislerin kesitleri incelendiğinde çok sayıda folikül içerdiği gözlenmiştir. Bu foliküllerde spermin gelişim aşamalarını içeren kistlere sahiptir. Foliküllerin distal uçlarından proksimal uçlara kadar sırasıyla germarium, büyüme bölgesi, olgunlaşma bölgesi ve transformasyon bölgesi bulunur. Bu aşamalarda spermatogonium, spermatosit, spermatidler ve son olarak spermatozoa oluşur. Yardımcı bezler, iki farklı boyut ve çapta içi boş boru şeklindeki yapılardır. Her iki yardımcı bez grubunda da tek katmanlı kübik veya piramidal hücreler lümeni çevreler. Bu yeni verilerin, B. turkiyae'nin erkek üreme sisteminin yapısı hakkındaki bilgilerimize katkıda bulunacağı umulmaktadır.

Keywords

Endemik tür, Böcek, Elektron mikroskopi, Morfoloji, Üreme sistemi

Project Number

FF080120B02

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