Horozibiği (Amaranthus Albus L.) Bitkisinde Putresin Uygulamasının Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Etkisi

Year 2025, Volume: 15 Issue: 1, 343 - 352, 01.03.2025

Tülay Toprak , Rüveyde Tunçtürk , Murat Tunçtürk

https://doi.org/10.21597/jist.1564573

Abstract

Bu çalışma; insan gıdası, kaba yem ve yağı için yetiştirilen ve son yıllarda üzerinde yoğun araştırmalar yapılan horoz ibiği (Amaranthus albus L.) bitkisinde putresin uygulamalarının bazı fizyolojik parametreler üzerine etkilerini belirlemek amacıyla yürütülmüştür. Çalışma 2022 yılında Van YYÜ Ziraat Fakültesi Tarla Bitkileri Bölümü’ ne ait tam kontrollü iklim odasında Tesadüf Parselleri Deneme Deseni’ ne göre faktöriyel düzende kurulmuştur. Dört tekerrürlü olarak kurulan denemede horoz ibiği bitkisi ve 4 farklı putresin dozu (0, 50, 100 ve 150 mM) kullanılmıştır. Araştırmada, toplam antioksidan aktivite, toplam fenolik madde, toplam flavonoid madde, askorbik asit, toplam karotenoid, malonildialdehit (MDA), klorofil a, b ve toplam klorofil içeriği gibi parametreler incelenmiştir. Araştırma sonucunda; putresin dozlarının klorofil a ve toplam klorofil miktarları hariç incelenen diğer parametreler üzerindeki etkisi istatistiksel olarak önemli bulunmuştur. Çalışmada, en yüksek antioksidan aktivite miktarı (19.66 µmol TE/ gTA), toplam fenolik madde miktarı (108.0 mg GAE /gTA), toplam flavonoid madde miktarı (130.37 mg QE /100gTA), askorbik asit içeriği (22.58 mg LAA /100gTA) ve toplam karotenoid içeriği (5.35 μg/g TA) 50 mM putresin uygulamalarından, en yüksek MDA içeriği kontrol parsellerinden ve en yüksek klorofil b içeriği (13.76 μg/g TA) 100 mM putresin uygulamalarından tespit edilmiştir. En düşük değerler ise genel olarak kontrol parsellerinden belirlenmiştir. Putresin uygulamaları kontrole kıyasla incelenen parametreler üzerinde olumlu ve etkili olmuştur. Bitki üzerinde kullanılan dozların herhangi bir strese neden olmadığı, MDA miktarının, kontrole göre doz arttıkça azaldığı belirlenmiştir.

Keywords

Biyokimyasal-madde miktarı, horoz ibiği, klorofil, putresin, tıbbi bitkiler

Effects of Putrescine Applications on Some Physiological and Biochemical Parameters in amaranth (Amaranthus Albus L.) plant

Abstract

This study was conducted to determine the effects of putrescine applications on some physiological parameters in Amaranthus albus L., a plant that is grown for human food, fodder or oil and has been the subject of intensive research in recent years. The study was set up in a factorial design based on the Randomized Complete Block Design with four replications in a fully controlled climate chamber at the Department of Field Crops, Faculty of Agriculture, Van Yuzuncu Yil University, in 2022. In the experiment, Amaranthus albus L. and four different putrescine doses (0, 50, 100, and 150 mM) were used. The parameters investigated included total antioxidant activity, total phenolic content, total flavonoid content, ascorbic acid, total carotenoid, malondialdehyde (MDA), chlorophyll a, chlorophyll b, and total chlorophyll content. As a result of the study, it was found that the effect of putrescine doses on the investigated parameters, except for chlorophyll a and total chlorophyll content, was statistically significant. The highest antioxidant activity (19.66 µmol TE/ g), total phenolic content (108.0 mg GAE /g), total flavonoid content (130.37 mg QE /100g), ascorbic acid content (22.58 mg LAA /100g), and total carotenoid content (5.35 μg/g) were determined in the 50 mM putrescine treatments. The highest MDA content was found in the control plots, while the highest chlorophyll b content (13.76 μg/g) was observed in the 100 mM putrescine treatments. The lowest values were generally observed in the control plots. Putrescine treatments had a positive and effective impact on the investigated parameters compared to the control. The fact that the MDA content decreased as the dose increased compared to the control indicates that the doses used on the plant did not cause any stress..

Keywords

Biochemical content, amaranthus albus L., chlorophyll, putrescine, medicinal plant

References

• Abd Elbar, O. H., Farag, R. E., & Shehata, S. A. (2019). Effect of putrescine application on some growth, biochemical and anatomical characteristics of Thymus vulgaris L. under drought stress. Annals of Agricultural Sciences, 64(2), 129-137.
• Abdel-Azem, H. S., Shehata, S. M., El-Gizawy, A. M., El-Yazied, A. A., & Adam, S. M. (2015). Snap bean response to salicylic acid and putrescine used separately and jointly under two sowing dates. Middle East Journal of Applied Sciences, 5(4), 1211-1221.
• Alasalvar, C., Grigor, J. M., Zhang, D., Quantick, P. C., & Shahidi, F. (2001). Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. Journal of Agricultural and Food Chemistry, 9(3), 1410-1416.
• Alizadeh, B., Ghahremani, Z., Barzegar, T., & Nikbakht, J. (2017). Effect of foliar application of putrescine on growth, yield and fruit quality of sweet pepper (Capsicum annum cv. Dimaz) under water stress. Journal of Crops Improvement, 19(2), 431-444.
• Amicarelli, V., & Camaggi, G. (2013). Amaranthus: A crop to rediscover. Forum Ware International, 2.
• Amin, A. A., Gharib, F. A. E., El-Awadi, M., & El-Sherbeny, M. R. (2011). Physiological response of onion to foliar application of putrescine and glutamine. Scientia Horticulturae, 129, 353-360.
• AOAC. (1990). Official methods of analysis (15th ed.). Association of Official Analytical Chemists Arlington, Virginia, USA. https://www.scirp.org/reference/ReferencesPapers?ReferenceID=1929875
• Bal, E. (2012). Hasat sonrası putresin ve salisilik asit uygulamalarının kirazın soğukta muhafazası üzerine etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7(2), 23-31.
• Benzie, I., Szeto, Y., Strain, J., & Tomlinson, B. (1999). Consumption of green tea causes rapid increase in plasma antioxidant power in humans. Nutrition and Cancer, 34(1), 83-87.
• Davarynejad, G. H., Zarei, M., Ardakani, E., & Nasrabadi, E. M. (2013). Influence of putrescine application on storability, postharvest quality and antioxidant activity of two Iranian apricot (Prunus armeniaca L.) cultivars. Notulae Scientia Biologicae, 5(2), 212-219.
• Davarynejad, G. H., Zarei, M., & Nasrabadi, M. E. (2015). Salisilik asit ve putresinin erik cv. 'Santa Rosa'nın depolanabilirliği, kalite özellikleri ve antioksidan aktivitesi üzerindeki etkileri. Journal of Food Science and Technology, 52, 2053–2062.
• Davies, P. J. (1995). In P. J. Davies (Ed.), Plant hormones: Physiology, biochemistry and molecular biology (2nd ed., pp. 1-12). Kluwer, Dordrecht, Netherlands.
• Davies, K. J. A. (2000). Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. International Union of Biochemistry and Molecular Biology Life, 50, 279-289.
• Ekinci, M., Yıldırım, E., Dursun, A., & Mohamedsrajaden, N. (2019). Putrescine, spermine and spermidine mitigated the salt stress damage on pepper (Capsicum annum L.) seedling. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 29(2), 290-299.
• Ergun, M., Özbay, N., Osmanoğlu, A., & Çalkır, A. (2014). Sebze ve tahıl olarak amarant (Amaranthus spp.) bitkisi. Iğdır Üniversitesi Fen Bilimleri Dergisi, 4(3), 21-28.
• Galston, A. W., & Kaur-Sawhney, R. (1995). Polyamines as endogenous growth regulators. In P. J. Davies (Ed.), Plant hormones: Physiology, biochemistry and molecular biology (2nd ed., pp. 158–178). Kluwer Academic Press, Dordrecht.
• Garcia-Lafuente, A., Guillamon, E., Villares, A., Rostagno, M. A., & Martinez, J. A. (2009). Flavonoids as anti-inflammatory agents: Implications in cancer and cardiovascular disease. Inflammation Research, 58, 537–552.
• Güler, H. D. (2019). Biberiye, fesleğen, kekik, nane ve stevyanın toplam fenolik madde ve antioksidan aktivitesi üzerine kurutma yöntemlerinin etkisi (Yüksek lisans tezi). Bursa Uludağ Üniversitesi, Fen Bilimleri Enstitüsü. https://acikerisim.uludag.edu.tr/items/5a19cff8-8e5a-481e-a2b2-6d3843c90895
• Halliwell, B., & Gutteridge, J. M. C. (1989). Free radicals in biology and medicine (pp. 238-240). Clarendon Press, Oxford.
• Hasanov, M. (2023). Tuz stresine maruz bırakılan çilek bitkisinde farklı putresin dozlarının fizyolojik faaliyetler üzerine etkileri (Yüksek lisans tezi). Bolu Abant İzzet Baysal Üniversitesi, Bahçe Bitkileri Anabilim Dalı. https://tez.yok.gov.tr/UlusalTezMerkezi
• Hasler, C. M. (2000). Plants as medicine: The role of phytochemicals in optimal health. In F. Shahidi & C.-T. Ho (Eds.), Phytochemicals and phytopharmaceuticals (pp. 1-12). AOAC Press, Champaign, Illinois.
• Heath, R. L., & Packer, L. (1968). İzole kloroplastlarda fotoperoksidasyon: I. Yağ asidi peroksidasyonunun kinematiği ve stokiyometrisi. Biyokimya ve Biyofizik Arşivleri, 125, 189-198.
• Islam, M. J., Uddin, M. J., Hossain, M. A., Henry, R., Begum, M. K., Sohel, M. A. T., ... & Lim, Y. S. (2022). Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.). PLOS One, 17(1).
• Kasnak, C., & Palamutoğlu, R. (2015). Doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri. Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 3(5), 226-234.
• Kao, C. H. (1997). Physiological significance of stress-induced changes in polyamines in plants. Botanical Bulletin of Academia Sinica, 38, 141-144.
• Kaya, A., & Doğanlar, Z. B. (2016). Exogenous jasmonic acid induces stress tolerance in tobacco (Nicotiana tabacum) exposed to imazapic. Ecotoxicology and Environmental Safety.
• Kibar, B., Şahin, B., & Kıemde, O. (2020). Fasulyede (Phaseolus vulgaris L.) farklı tuz ve putresin uygulamalarının çimlenme ve fide gelişimi üzerine etkileri. Journal of the Institute of Science and Technology, 10(4), 2315-2327.
• Koca, N., & Karadeniz, F. (2005). Gıdalardaki doğal antioksidan bileşikler. Gıda, 30(4), 229-236.
• Lichtenthaler, H. K., & Welburn, A. R. (1985). Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biochemical Society Transactions, 11, 591-592.
• Liu, K., Fu, H. H., Bei, Q. X., & Luan, S. (2000). Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements. Plant Physiology, 124, 1315–1325.
• Lutz, M., Jorquera, K., Cancino, B., Ruby, R., & Henriquez, C. (2011). Phenolics and antioxidant capacity of table grape (Vitis vinifera L.) cultivars grown in Chile. Journal of Food Science, 76, 1088-1093. https://doi.org/10.1111/j.1750-3841.2011.02298.x
• Mirdehghan, S. H., Rahemi, M., Castillo, S., Martínez-Romero, D., Serrano, M., & Valero, D. (2007). Pre-storage application of polyamines by pressure or immersion improves shelf-life of pomegranate stored at chilling temperature by increasing endogenous polyamine levels. Postharvest Biology and Technology, 44(1), 26-33.
• Mohamedsrajaden, N. S. (2019). Poliaminlerin tuzlu şartlarda domateste çimlenme, fide gelişimi, antioksidan enzim aktivitesi ve mineral madde içeriği üzerine etkisi (Yüksek lisans tezi). Atatürk Üniversitesi Fen Bilimleri Enstitüsü. https://tez.yok.gov.tr/UlusalTezMerkezi
• Obanda, M., Owuor, P. O., & Taylor, S. J. (1997). Flavanol composition and caffeine content of green leaf as.
• Oğurlu, F. (2023). Armut meyvelerinin soğukta muhafazasına modifiye atmosfer paketleme, aloe vera kaplama ve putresin uygulamalarının etkisi (Yüksek lisans tezi). Siirt Üniversitesi Fen Bilimleri Enstitüsü. https://tez.yok.gov.tr/UlusalTezMerkezi
• Ötleş, S., & Atlı, Y. (1997). Karotenoidlerin insan sağlığı açısından önemi. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, 3(1), 249-254.
• Özaslan, C., & Kendal, E. (2014). Lice domatesi üretim alanlarındaki yabancı otların belirlenmesi. Journal of the Institute of Science and Technology, 4(3), 29-34.
• Padayatty, S. J., Katz, A., & Wang, Y. (2003). Vitamin C as an antioxidant: Evaluation of its role in disease prevention. Journal of the American College of Nutrition, 22, 18-35.
• Pimentel, D., Marklein, A., Toth, M. A., Karpoff, M., Paul, G. S., McCormack, R., Kyriazis, J., & Krueger, T. (2008). Biofuel impacts on world food supply: Use of fossil fuel, land and water resources. Energies, 1, 41-78.
• Prakash, L. G., & Prathapasenan, G. (1988). Putrescine reduces NaCl-induced inhibition of germination and early seedling growth of rice (Oryza sativa L.). Australian Journal of Plant Physiology, 15, 761–767.
• Putnam, D. H., Oplinger, E. S., Doll, J. D., & Schulte, E. M. (2014). Amarant. http://www.hort.purdue.edu/newcrop/afcm/amaranth.html (Erişim tarihi: 19.03.2014).
• Qing, X., Zhao, X., Hu, C., Wang, P., Zhang, Y., Zhang, X., Wang, P., Shi, H., Shi, H., Jia, F., & Qu, C. (2015). Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage (Brassica campestris L. ssp. Pekinensis) leaves. Ecotoxicology and Environmental Safety, 114, 179–189.
• Qiu, Z., Guo, J., Zhu, A., Zhang, L., & Zhang, M. (2014). Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress. Ecotoxicology and Environmental Safety, 104, 202-208.
• Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, J., Luyck, M., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72, 35-40.
• Quinet, P., Vinogradoff, V., Palmeri, P., & Biemont, E. (2010). J. Phys. B: At. Mol. Opt. Phys. 43, 144003.
• Razzaq, K., Khan, A. S., Malik, A. U., Shahid, M., & Ullah, S. (2014). Role of putrescine in regulating fruit softening and antioxidative enzyme systems in ‘Samar Bahisht Chaunsa’ mango. Postharvest Biology and Technology, 96, 23-32.
• Sairam, R. K., & Saxena, D. C. (2000). Oxidative stress and antioxidants in wheat genotypes: Possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science, 184(1), 55-61.
• Sardar, R., Ahmed, S., & Yasin, N. A. (2021). Antioksidan sistemi düzenleyerek Coriandrum sativum'daki kadmiyum stresinin iyileştirilmesinde ekzojen olarak uygulanan putrescinin rolü. Uluslararası Fitoremediasyon Dergisi, 24(9), 955–962. https://doi.org/10.1080/15226514.2021.1985961
• Schwinn, K. E., & Davies, K. M. (2004). Flavonoids. In K. Davies (Ed.), Plant pigments and their manipulation, Annual Plant Reviews (Vol. 14, pp. 92-149). Blackwell Publishing.
• Selzer, L. J., & Busso, C. A. (2016). Pigments and photosynthesis of understory grasses: Light irradiance and soil moisture effects. Russian Journal of Plant Physiology, 63, 224-234.
• Shi, H., Ye, T., & Chan, Z. (2013). Comparative proteomic and physiological analyses reveal the protective effect of exogenous polyamines in the Bermuda grass (Cynodon dactylon) response to salt and drought stresses. Journal of Proteome Research, 12, 4807-4829.
• Shu, S., Gua, S. R., Sun, J., & Yuan, L. Y. (2012). Effects of salt stress on the structure and function of the photosynthetic apparatus in Cucumis sativus and its protection by exogenous putrescine. Physiologia Plantarum, 146, 285-296.
• Slocum, R. D., & Flores, H. E. (Eds.). (1991). Biochemistry and physiology of polyamines in plants. CRC Press, Boca Raton, FL, USA.
• Tan, M., & Temel, S. (2012). Alternatif yem bitkileri. Atatürk Üniversitesi Ziraat Fakültesi Ders Yayınları.
• Tan, M., Koç, A., & Dumlu Gul, Z. (2012). Morphological characteristics and seed yield of East Anatolian local forage pea (Pisum sativum ssp. arvense L.) ecotypes. Turkish Journal of Field Crops, 17(1), 24-30.
• Tekin, F., & Bozcuk, S. (1998). Helianthus annuus L. var. Santefe (Ayçiçeği) tohumlarının çimlenmesi ve erken büyüme üzerine tuz ve dışsal putresinin etkileri. Turkish Journal of Biology, 22, 331-340.
• Venskutonis, P. C. R., & Kraujalis, P. (2013). Nutritional components of amaranth seeds and vegetables: A review on composition, properties, and uses. Comprehensive Reviews in Food Science and Food Safety, 12, 381-412.
• Wang, L., Lee, I. M., Zhang, S. M., Blumberg, J. B., Buring, J. E., & Sesso, H. D. (2009). Dietary intake of selected flavonols, flavones, and flavonoid-rich foods and risk of cancer in middle-aged and older women. American Journal of Clinical Nutrition, 89(3), 905–912.
• Xu, X., Shi, G., Ding, C., & Xu, Y. (2011). Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regulation, 63, 251-258.
• Yao, L. H., Jiang, Y. M., Shi, J., Tomas-Barberan, F. A., Datta, N., Singanusong, R., & Chen, S. S. (2004). Flavonoids in food and their health benefits. Plant Foods for Human Nutrition, 59, 113–122.
• Yıldız, M., Terzi, H., & Akçalı, N. (2014). Bitki tuz stresi toleransında salisilik asit ve poliaminler (021002) (7-22). Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 14(2), 7-22.
• Zeid, I. M. (2004). Response of bean (Phaseolus vulgaris) to exogenous putrescine treatment under salinity stress. Pakistan Journal of Biological Sciences, 7(2), 219-225.