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Sıçanlarda ve Farelerde Yaşam Alanı Kriterleri

Year 2024, Volume: 4 Issue: 1, 8 - 14, 29.03.2024
https://doi.org/10.5281/zenodo.10894313

Abstract

Rat ve fareler bilimsel çalışmalarda yaygın olarak kullanılan laboratuvar hayvanlarıdır. Rat ve farelerin bulundurulduğu ortam hayvanların yaşam alanı kriterlerine uygun olmalıdır. Hayvan sağlığı ve refahının sağlanamaması durumunda bilimsel çalışmalar etkilenir ve yanlış sonuçlar elde edilir. Yaşam alanı kriterlerinin ilk basamağı tesis tasarımıdır. Tesis rat ve fareler için uygun olacak şekilde tasarlanmalıdır. Rat ve farelerin bulundurulduğu odalar Laboratuvar Hayvanlarının Bakımı ve Kullanımı Kılavuzu'nun yönergelerine uygun olacak şekilde düzenlenmelidir. Oda içine yerleştirilecek kafesler çalışma amacına göre belirlenmekle birlikte kolay temizlenebilir, kaçmaya karşı korumalı olmalıdır. Çalışma için gerekli olmadığı sürece tel kafesler yerine katı tabanlı olan kafesler tercih edilmelidir. Kafes boyutları hayvan popülasyonuna göre belirlenmelidir. Sıcaklık, nem, hava hareketi, aydınlatma, sanitasyon ve gürültü, yem su ve altlık araştırma sonuçlarını etkileyen çevresel faktörlerdir. Rat ve farelerin bulunduğu ortam fizyolojilerine uygun sıcaklık ve nem derecesinde olmalı, Etkili bir havalandırma ve uygun bir fotoperiyod döngüsü sağlanmalı ve gürültüden uzak olmalıdır. Ayrıca sağlığa elverişli koşulların sürdürülmesi ve dezenfeksiyon için etkili bir sanitasyon belirlenmelidir. Yem, su ve altlık materyali de hayvan refahını etkileyen önemli çevresel faktörlerdir. Yem fare ve ratların ihtiyacına göre belirlenmeli, temiz su sağlanmalı ve en uygun altlık malzemesi kullanılmalıdır.

References

  • 1. Bailoo JD., Reichlin TS., Wuerbel H., 2014. Refinement of experimental design and conduct in laboratory animal research. ILAR journal, 55(3), 383-391.
  • 2. Baumans V., 2005. Science-based assessment of animal welfare: laboratory animals. Revue Scientifique Et Technique-Office International Des Epizooties, 24(2), 503.
  • 3. Blask DE., Dauchy RT., Dauchy EM., Mao L., Hill SM., Greene MW., Davidson L., 2014. Light exposure at night disrupts host/cancer circadian regulatory dynamics: impact on the Warburg effect, lipid signaling and tumor growth prevention. PLoS One, 9(8), e102776.
  • 4. Castelhano-Carlos MJ., Baumans V., 2009. The impact of light, noise, cage cleaning and in-house transport on welfare and stress of laboratory rats. Laboratory animals, 43(4), 311-327.
  • 5. Council NR., (2011). Guide fo the Care and Use of Laboratory Animals: Eight Edition. Tha National Academies Press, Washington, DC. [Google Scholar].
  • 6. Dauchy RT., Dauchy EM., Tirrell RP., Hill CR., Davidson LK., Greene MW., Blask DE., 2010. Dark-phase light contamination disrupts circadian rhythms in plasma measures of endocrine physiology and metabolism in rats. Comparative medicine, 60(5), 348-356.
  • 7. Demers G., Griffin G., De Vroey G., Haywood JR., Zurlo J., Bédard M., 2006. Harmonization of animal care and use guidance. Science, 312(5774), 700-701.
  • 8. Drucker DJ., 2016. Never waste a good crisis: confronting reproducibility in translational research. Cell metabolism, 24(3), 348-360.
  • 9. Faith RE., Huerkamp MJ. 2009. Environmental considerations for research animals. Planning and designing research animal facilities, 59-83.
  • 10. Faith RE., Allen KP., Hessler JR., 2020. Housing and environment. The laboratory rat, 349-417.
  • 11. Ferrecchia CE., Jensen K., Van Andel R., 2014. Intracage ammonia levels in static and individually ventilated cages housing C57BL/6 mice on 4 bedding substrates. Journal of the American Association for Laboratory Animal Science, 53(2), 146-151.
  • 12. Frohlich J., 2020. Rats and mice. Ferrets, Rabbits, and Rodents, 345.
  • 13. Hamilton BA., Frankel WN., 2001. Of mice and genome sequence. Cell, 107(1), 13-16.
  • 14. Hessler JR., Broderson R., King C., 1999. Animal research facilities and equipment. Anthology of Biosafety, 1, 191-217.
  • 15. Izraeli S., Rechavi G., 2002. Molecular medicine--an overview. The Israel Medical Association journal: IMAJ, 4(8), 638–640.
  • 16. Kwitek-Black, A. E., & Jacob, H. J. (2001). The use of designer rats in the genetic dissection of hypertension. Current hypertension reports, 3(1), 12-18.
  • 17. National Research Council [NRC], 2011. Guide for the Care and Use of Laboratory Animals, eighth ed. National Academy Press, Washington, DC.
  • 18. Otto GM., Franklin CL., Clifford CB., 2015. Biology and diseases of rats. In Laboratory animal medicine (pp. 151-207). Academic Press.
  • 19. Pascuan CG., Uran SL., Gonzalez-Murano MR., Wald MR., Guelman LR., Genaro AM., 2014. Immune alterations induced by chronic noise exposure: comparison with restraint stress in BALB/c and C57Bl/6 mice. Journal of immunotoxicology, 11(1), 78-83.
  • 20. Peace TA., Singer AW., Niemuth NA., Shaw ME., 2001. Effects of caging type and animal source on the development of foot lesions in Sprague Dawley rats (Rattus norvegicus). Journal of the American Association for Laboratory Animal Science, 40(5), 17-21.
  • 21. Turner, J., 2017. Noise and Vibration in the Vivarium. American Association for Laboratory Animal Science (AALAS). Webinar pre- sented September 6, 2017.
  • 22. Warthen DM., Provencio I., 2012. The role of intrinsically photosensitive retinal ganglion cells in nonimage-forming responses to light. Eye and brain, 4, 43.
  • 23. Wu, J., Dauchy, R. T., Tirrell, P. C., Wu, S. S., Lynch, D. T., Jitawatanarat, P., ... & Greene, M. W. (2011). Light at night activates IGF-1R/PDK1 signaling and accelerates tumor growth in human breast cancer xenografts. Cancer research, 71(7), 2622-2631.

Habitat Criteria in Rats and Mice

Year 2024, Volume: 4 Issue: 1, 8 - 14, 29.03.2024
https://doi.org/10.5281/zenodo.10894313

Abstract

Rats and mice are laboratory animals commonly used in scientific studies. The environment in which rats and mice are kept must comply with the criteria for the habitat of the animals. If animal health and welfare are not ensured, scientific studies are affected and false results are obtained. The first step of the living space criteria is facility design. The facility should be designed to be suitable for rats and mice. The rooms where rats and mice are kept should be arranged in accordance with the guidelines of the Laboratory Animal Care and Use Guide. Although the cages to be placed in the room are determined according to the working purpose, they should be easy to clean and protected against escape. Unless necessary for the study, cages with solid bases should be preferred over wire cages. Cage sizes should be determined according to the animal population. Temperature, humidity, air movement, lighting, sanitation and noise, feed water and litter are environmental factors that affect research results. The environment where rats and mice are located should be at a temperature and humidity level suitable for their physiology, An effective ventilation and a suitable photoperiod cycle should be provided and it should be away from noise. In addition, an effective sanitation should be determined for the maintenance of sanitary conditions and disinfection. Feed, water and litter material are also important environmental factors affecting animal welfare. Feed should be determined according to the needs of mice and rats, clean water should be provided and the most suitable litter material should be used.

References

  • 1. Bailoo JD., Reichlin TS., Wuerbel H., 2014. Refinement of experimental design and conduct in laboratory animal research. ILAR journal, 55(3), 383-391.
  • 2. Baumans V., 2005. Science-based assessment of animal welfare: laboratory animals. Revue Scientifique Et Technique-Office International Des Epizooties, 24(2), 503.
  • 3. Blask DE., Dauchy RT., Dauchy EM., Mao L., Hill SM., Greene MW., Davidson L., 2014. Light exposure at night disrupts host/cancer circadian regulatory dynamics: impact on the Warburg effect, lipid signaling and tumor growth prevention. PLoS One, 9(8), e102776.
  • 4. Castelhano-Carlos MJ., Baumans V., 2009. The impact of light, noise, cage cleaning and in-house transport on welfare and stress of laboratory rats. Laboratory animals, 43(4), 311-327.
  • 5. Council NR., (2011). Guide fo the Care and Use of Laboratory Animals: Eight Edition. Tha National Academies Press, Washington, DC. [Google Scholar].
  • 6. Dauchy RT., Dauchy EM., Tirrell RP., Hill CR., Davidson LK., Greene MW., Blask DE., 2010. Dark-phase light contamination disrupts circadian rhythms in plasma measures of endocrine physiology and metabolism in rats. Comparative medicine, 60(5), 348-356.
  • 7. Demers G., Griffin G., De Vroey G., Haywood JR., Zurlo J., Bédard M., 2006. Harmonization of animal care and use guidance. Science, 312(5774), 700-701.
  • 8. Drucker DJ., 2016. Never waste a good crisis: confronting reproducibility in translational research. Cell metabolism, 24(3), 348-360.
  • 9. Faith RE., Huerkamp MJ. 2009. Environmental considerations for research animals. Planning and designing research animal facilities, 59-83.
  • 10. Faith RE., Allen KP., Hessler JR., 2020. Housing and environment. The laboratory rat, 349-417.
  • 11. Ferrecchia CE., Jensen K., Van Andel R., 2014. Intracage ammonia levels in static and individually ventilated cages housing C57BL/6 mice on 4 bedding substrates. Journal of the American Association for Laboratory Animal Science, 53(2), 146-151.
  • 12. Frohlich J., 2020. Rats and mice. Ferrets, Rabbits, and Rodents, 345.
  • 13. Hamilton BA., Frankel WN., 2001. Of mice and genome sequence. Cell, 107(1), 13-16.
  • 14. Hessler JR., Broderson R., King C., 1999. Animal research facilities and equipment. Anthology of Biosafety, 1, 191-217.
  • 15. Izraeli S., Rechavi G., 2002. Molecular medicine--an overview. The Israel Medical Association journal: IMAJ, 4(8), 638–640.
  • 16. Kwitek-Black, A. E., & Jacob, H. J. (2001). The use of designer rats in the genetic dissection of hypertension. Current hypertension reports, 3(1), 12-18.
  • 17. National Research Council [NRC], 2011. Guide for the Care and Use of Laboratory Animals, eighth ed. National Academy Press, Washington, DC.
  • 18. Otto GM., Franklin CL., Clifford CB., 2015. Biology and diseases of rats. In Laboratory animal medicine (pp. 151-207). Academic Press.
  • 19. Pascuan CG., Uran SL., Gonzalez-Murano MR., Wald MR., Guelman LR., Genaro AM., 2014. Immune alterations induced by chronic noise exposure: comparison with restraint stress in BALB/c and C57Bl/6 mice. Journal of immunotoxicology, 11(1), 78-83.
  • 20. Peace TA., Singer AW., Niemuth NA., Shaw ME., 2001. Effects of caging type and animal source on the development of foot lesions in Sprague Dawley rats (Rattus norvegicus). Journal of the American Association for Laboratory Animal Science, 40(5), 17-21.
  • 21. Turner, J., 2017. Noise and Vibration in the Vivarium. American Association for Laboratory Animal Science (AALAS). Webinar pre- sented September 6, 2017.
  • 22. Warthen DM., Provencio I., 2012. The role of intrinsically photosensitive retinal ganglion cells in nonimage-forming responses to light. Eye and brain, 4, 43.
  • 23. Wu, J., Dauchy, R. T., Tirrell, P. C., Wu, S. S., Lynch, D. T., Jitawatanarat, P., ... & Greene, M. W. (2011). Light at night activates IGF-1R/PDK1 signaling and accelerates tumor growth in human breast cancer xenografts. Cancer research, 71(7), 2622-2631.
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Sciences
Journal Section Reviews
Authors

Sümeyye Baysal 0000-0003-1464-4993

Emre Eren 0000-0003-3118-7384

Emre Yanar 0000-0001-7302-7077

Publication Date March 29, 2024
Submission Date January 4, 2023
Published in Issue Year 2024 Volume: 4 Issue: 1

Cite

EndNote Baysal S, Eren E, Yanar E (March 1, 2024) Sıçanlarda ve Farelerde Yaşam Alanı Kriterleri. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi 4 1 8–14.

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